Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure
A sequencing batch reactor (SBR) with an intermittent aeration mode was established to achieve partial nitrification (PN) and the short-term effects of C/N ratios were investigated. Stable nitrite accumulation was achieved after 107 cycles, about 56d, with the average ammonia nitrogen removal effici...
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doaj-b9a3e937abc4403cb6a3f8fc49bf8a482020-12-12T00:02:45ZengMDPI AGWater2073-44412020-12-01123485348510.3390/w12123485Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community StructureZhengyao Zhou0Meixia Qi1Hongping Wang2Department of Environmental Engineering, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, ChinaDepartment of Environmental Engineering, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, ChinaDepartment of Environmental Engineering, School of Resource and Environmental Science, Wuhan University, Wuhan 430079, ChinaA sequencing batch reactor (SBR) with an intermittent aeration mode was established to achieve partial nitrification (PN) and the short-term effects of C/N ratios were investigated. Stable nitrite accumulation was achieved after 107 cycles, about 56d, with the average ammonia nitrogen removal efficiency (ARE) and nitrite accumulation rate (NAR) of 96.92% and 82.49%, respectively. When the C/N ratios decreased from 4.64 to 3.87 and 2.32, ARE and NAR still kept a stable and high level. However, when the C/N ratio further decreased to 0.77, nitrite accumulation became fluctuation, and ARE, total nitrogen (TN), and chemical oxygen demand (COD) removal performance declined obviously. Except for four common phyla (<i>Proteobacteria</i>, <i>Bacteroidetes</i>, <i>Chloroflexi,</i> and <i>Acidobacteria</i>) in the wastewater treatment system, <i>Patescibacteria</i>, the newly defined superphylum, was found and became the most dominant phylum in the PN sludge for their ultra-small cell size. The only ammonia oxidation bacteria (AOB), <i>Nitrosomonas</i>, and nitrite oxidation bacteria (NOB), <i>Nitrospira</i>, were detected. The relative abundance of NOB was low at different C/N ratios, showing the stable and effective inhibition effects of intermittent aeration on NOB growth.https://www.mdpi.com/2073-4441/12/12/3485partial nitrificationintermittent aerationC/N ratiohigh-throughput sequencingmicrobial community |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Zhengyao Zhou Meixia Qi Hongping Wang |
spellingShingle |
Zhengyao Zhou Meixia Qi Hongping Wang Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure Water partial nitrification intermittent aeration C/N ratio high-throughput sequencing microbial community |
author_facet |
Zhengyao Zhou Meixia Qi Hongping Wang |
author_sort |
Zhengyao Zhou |
title |
Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure |
title_short |
Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure |
title_full |
Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure |
title_fullStr |
Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure |
title_full_unstemmed |
Achieving Partial Nitrification via Intermittent Aeration in SBR and Short-Term Effects of Different C/N Ratios on Reactor Performance and Microbial Community Structure |
title_sort |
achieving partial nitrification via intermittent aeration in sbr and short-term effects of different c/n ratios on reactor performance and microbial community structure |
publisher |
MDPI AG |
series |
Water |
issn |
2073-4441 |
publishDate |
2020-12-01 |
description |
A sequencing batch reactor (SBR) with an intermittent aeration mode was established to achieve partial nitrification (PN) and the short-term effects of C/N ratios were investigated. Stable nitrite accumulation was achieved after 107 cycles, about 56d, with the average ammonia nitrogen removal efficiency (ARE) and nitrite accumulation rate (NAR) of 96.92% and 82.49%, respectively. When the C/N ratios decreased from 4.64 to 3.87 and 2.32, ARE and NAR still kept a stable and high level. However, when the C/N ratio further decreased to 0.77, nitrite accumulation became fluctuation, and ARE, total nitrogen (TN), and chemical oxygen demand (COD) removal performance declined obviously. Except for four common phyla (<i>Proteobacteria</i>, <i>Bacteroidetes</i>, <i>Chloroflexi,</i> and <i>Acidobacteria</i>) in the wastewater treatment system, <i>Patescibacteria</i>, the newly defined superphylum, was found and became the most dominant phylum in the PN sludge for their ultra-small cell size. The only ammonia oxidation bacteria (AOB), <i>Nitrosomonas</i>, and nitrite oxidation bacteria (NOB), <i>Nitrospira</i>, were detected. The relative abundance of NOB was low at different C/N ratios, showing the stable and effective inhibition effects of intermittent aeration on NOB growth. |
topic |
partial nitrification intermittent aeration C/N ratio high-throughput sequencing microbial community |
url |
https://www.mdpi.com/2073-4441/12/12/3485 |
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