Periostin Mediates Oestrogen-Induced Osteogenic Differentiation of Bone Marrow Stromal Cells in Ovariectomised Rats

Periostin Mediates Oestrogen-Induced Osteogenic Differentiation of Bone Marrow Stromal Cells in Ovariectomised Rats

Osteoporosis is a metabolic disease that results in the progressive loss of bone mass, which, in postmenopausal women, is related to oestrogen deficiency. Periostin (POSTN) plays a key role in the early stages of bone formation. However, whether POSTN participates in oestradiol regulation of osteoge...

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Main Authors: Chunrong Li, Xin Li, Xian Wang, Pei Miao, Jia Liu, Cuixia Li, Doudou Li, Weiwei Zhou, Zuolin Jin, Meng Cao
Format: Article
Language:English
Published: Hindawi Limited 2020-01-01
Series:BioMed Research International
Online Access:http://dx.doi.org/10.1155/2020/9405909
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record_format Article
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language English
format Article
sources DOAJ
author Chunrong Li
Xin Li
Xian Wang
Pei Miao
Jia Liu
Cuixia Li
Doudou Li
Weiwei Zhou
Zuolin Jin
Meng Cao
spellingShingle Chunrong Li
Xin Li
Xian Wang
Pei Miao
Jia Liu
Cuixia Li
Doudou Li
Weiwei Zhou
Zuolin Jin
Meng Cao
Periostin Mediates Oestrogen-Induced Osteogenic Differentiation of Bone Marrow Stromal Cells in Ovariectomised Rats
BioMed Research International
author_facet Chunrong Li
Xin Li
Xian Wang
Pei Miao
Jia Liu
Cuixia Li
Doudou Li
Weiwei Zhou
Zuolin Jin
Meng Cao
author_sort Chunrong Li
title Periostin Mediates Oestrogen-Induced Osteogenic Differentiation of Bone Marrow Stromal Cells in Ovariectomised Rats
title_short Periostin Mediates Oestrogen-Induced Osteogenic Differentiation of Bone Marrow Stromal Cells in Ovariectomised Rats
title_full Periostin Mediates Oestrogen-Induced Osteogenic Differentiation of Bone Marrow Stromal Cells in Ovariectomised Rats
title_fullStr Periostin Mediates Oestrogen-Induced Osteogenic Differentiation of Bone Marrow Stromal Cells in Ovariectomised Rats
title_full_unstemmed Periostin Mediates Oestrogen-Induced Osteogenic Differentiation of Bone Marrow Stromal Cells in Ovariectomised Rats
title_sort periostin mediates oestrogen-induced osteogenic differentiation of bone marrow stromal cells in ovariectomised rats
publisher Hindawi Limited
series BioMed Research International
issn 2314-6133
2314-6141
publishDate 2020-01-01
description Osteoporosis is a metabolic disease that results in the progressive loss of bone mass, which, in postmenopausal women, is related to oestrogen deficiency. Periostin (POSTN) plays a key role in the early stages of bone formation. However, whether POSTN participates in oestradiol regulation of osteogenic differentiation of bone marrow stromal cells (BMSCs) from ovariectomised (OVX) rats remains unclear. In vivo, using microcomputed tomography (micro-CT), immunohistochemistry, and dynamic analysis of femurs, we found that 17β-E2 promotes bone formation and POSTN expression at the endosteal surface. In vitro, 17β-E2 upregulated POSTN expression in OVX-BMSCs. POSTN overexpression activated the Wnt/β-catenin signalling pathway and enhanced osteogenic differentiation of OVX-BMSCs. Furthermore, knockdown of Postn blocks the involvement of 17β-E2 in the osteogenic differentiation of OVX-BMSCs. Collectively, our study indicated the role of POSTN in the osteogenesis and stemness of OVX-BMSCs and proves that 17β-E2 reduces osteoporosis and promotes osteogenesis through the POSTN-Wnt/β-catenin pathway. POSTN could, therefore, be a novel target gene for anti-osteoporosis therapies.
url http://dx.doi.org/10.1155/2020/9405909
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spelling doaj-b9ef7374d64146a68e17a9ad58bd80302020-11-25T03:02:59ZengHindawi LimitedBioMed Research International2314-61332314-61412020-01-01202010.1155/2020/94059099405909Periostin Mediates Oestrogen-Induced Osteogenic Differentiation of Bone Marrow Stromal Cells in Ovariectomised RatsChunrong Li0Xin Li1Xian Wang2Pei Miao3Jia Liu4Cuixia Li5Doudou Li6Weiwei Zhou7Zuolin Jin8Meng Cao9State Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Clinical Research Centre for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, Shaanxi 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Clinical Research Centre for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, Shaanxi 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Clinical Research Centre for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, Shaanxi 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Clinical Research Centre for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, Shaanxi 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Clinical Research Centre for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, Shaanxi 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Clinical Research Centre for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, Shaanxi 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Clinical Research Centre for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, Shaanxi 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Clinical Research Centre for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, Shaanxi 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Clinical Research Centre for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, Shaanxi 710032, ChinaState Key Laboratory of Military Stomatology & National Clinical Research Centre for Oral Diseases & Shaanxi Clinical Research Centre for Oral Diseases, Department of Orthodontics, School of Stomatology, The Fourth Military Medical University, 145 West Changle Road, Xi’an, Shaanxi 710032, ChinaOsteoporosis is a metabolic disease that results in the progressive loss of bone mass, which, in postmenopausal women, is related to oestrogen deficiency. Periostin (POSTN) plays a key role in the early stages of bone formation. However, whether POSTN participates in oestradiol regulation of osteogenic differentiation of bone marrow stromal cells (BMSCs) from ovariectomised (OVX) rats remains unclear. In vivo, using microcomputed tomography (micro-CT), immunohistochemistry, and dynamic analysis of femurs, we found that 17β-E2 promotes bone formation and POSTN expression at the endosteal surface. In vitro, 17β-E2 upregulated POSTN expression in OVX-BMSCs. POSTN overexpression activated the Wnt/β-catenin signalling pathway and enhanced osteogenic differentiation of OVX-BMSCs. Furthermore, knockdown of Postn blocks the involvement of 17β-E2 in the osteogenic differentiation of OVX-BMSCs. Collectively, our study indicated the role of POSTN in the osteogenesis and stemness of OVX-BMSCs and proves that 17β-E2 reduces osteoporosis and promotes osteogenesis through the POSTN-Wnt/β-catenin pathway. POSTN could, therefore, be a novel target gene for anti-osteoporosis therapies.http://dx.doi.org/10.1155/2020/9405909

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