Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solution Demonstrate Equal Effectiveness in the Preservation of Human Pancreata Intended for Islet Isolation: A Large-Scale, Single-Center Experience
We previously reported a small-scale study on the efficacy of histidine-tryptophan-ketoglutarate (HTK) solution versus University of Wisconsin (UW) solution on pancreas preservation for islet isolation. In this large-scale, retrospective analysis ( n = 252), we extend our initial description of the...
Main Authors: | , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
SAGE Publishing
2013-07-01
|
Series: | Cell Transplantation |
Online Access: | https://doi.org/10.3727/096368912X657332 |
id |
doaj-bbf422f6ffd4453cbb722721bddb5eb9 |
---|---|
record_format |
Article |
spelling |
doaj-bbf422f6ffd4453cbb722721bddb5eb92020-11-25T03:02:54ZengSAGE PublishingCell Transplantation0963-68971555-38922013-07-012210.3727/096368912X657332Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solution Demonstrate Equal Effectiveness in the Preservation of Human Pancreata Intended for Islet Isolation: A Large-Scale, Single-Center ExperienceDaniel H. Paushter0Meirigeng Qi1Kirstie K. Danielson2Tricia A. Harvat3Katie Kinzer4Barbara Barbaro5Sonny Patel6Sarah Z. Hassan7Jose Oberholzer8Yong Wang9Department of Transplant/Surgery, University of Illinois at Chicago, Chicago, IL, USADepartment of Transplant/Surgery, University of Illinois at Chicago, Chicago, IL, USADepartment of Transplant/Surgery, University of Illinois at Chicago, Chicago, IL, USADepartment of Transplant/Surgery, University of Illinois at Chicago, Chicago, IL, USADepartment of Transplant/Surgery, University of Illinois at Chicago, Chicago, IL, USADepartment of Transplant/Surgery, University of Illinois at Chicago, Chicago, IL, USADepartment of Transplant/Surgery, University of Illinois at Chicago, Chicago, IL, USADepartment of Transplant/Surgery, University of Illinois at Chicago, Chicago, IL, USADepartment of Transplant/Surgery, University of Illinois at Chicago, Chicago, IL, USADepartment of Transplant/Surgery, University of Illinois at Chicago, Chicago, IL, USAWe previously reported a small-scale study on the efficacy of histidine-tryptophan-ketoglutarate (HTK) solution versus University of Wisconsin (UW) solution on pancreas preservation for islet isolation. In this large-scale, retrospective analysis ( n = 252), we extend our initial description of the impact of HTK on islet isolation outcomes and include pancreatic digestion efficacy, purification outcomes, and islet size distribution. Multivariable linear regression analysis, adjusted for donor age, sex, BMI, cold ischemia time, and enzyme, demonstrated similar results for the HTK group ( n = 95) and the UW group ( n = 157), including postpurification islet yields (HTK: 289,702 IEQ vs. UW: 283,036 IEQ; p = 0.76), percentage of digested pancreatic tissue (HTK: 66.9% vs. UW: 64.1%; p = 0.18), and islet loss from postdigestion to postpurification (HTK: 24,972 IEQ vs. UW: 39,551 IEQ; p = 0.38). Changes in islet size between the postdigestion and postpurification stages were comparable within each islet size category for HTK and UW ( p = 0.14–0.99). Tissue volume distribution across purification fractions and islet purity in the top fractions were similar between the groups; however, the HTK group had significantly higher islet purity in the middle fractions ( p = 0.003–0.008). Islet viability and stimulation indices were also similar between the HTK and the UW groups. In addition, we analyzed a small sample of patients transplanted either with HTK ( n = 7) or UW ( n = 8) preserved islets and found no significant differences in posttransplant HbA 1c , β-score, and frequency of insulin independence. This study demonstrates that HTK and UW solutions offer comparable pancreas preservation for islet transplantation. More in vivo islet outcome data are needed for a complete analysis of the effects of HTK on islet transplantation.https://doi.org/10.3727/096368912X657332 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniel H. Paushter Meirigeng Qi Kirstie K. Danielson Tricia A. Harvat Katie Kinzer Barbara Barbaro Sonny Patel Sarah Z. Hassan Jose Oberholzer Yong Wang |
spellingShingle |
Daniel H. Paushter Meirigeng Qi Kirstie K. Danielson Tricia A. Harvat Katie Kinzer Barbara Barbaro Sonny Patel Sarah Z. Hassan Jose Oberholzer Yong Wang Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solution Demonstrate Equal Effectiveness in the Preservation of Human Pancreata Intended for Islet Isolation: A Large-Scale, Single-Center Experience Cell Transplantation |
author_facet |
Daniel H. Paushter Meirigeng Qi Kirstie K. Danielson Tricia A. Harvat Katie Kinzer Barbara Barbaro Sonny Patel Sarah Z. Hassan Jose Oberholzer Yong Wang |
author_sort |
Daniel H. Paushter |
title |
Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solution Demonstrate Equal Effectiveness in the Preservation of Human Pancreata Intended for Islet Isolation: A Large-Scale, Single-Center Experience |
title_short |
Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solution Demonstrate Equal Effectiveness in the Preservation of Human Pancreata Intended for Islet Isolation: A Large-Scale, Single-Center Experience |
title_full |
Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solution Demonstrate Equal Effectiveness in the Preservation of Human Pancreata Intended for Islet Isolation: A Large-Scale, Single-Center Experience |
title_fullStr |
Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solution Demonstrate Equal Effectiveness in the Preservation of Human Pancreata Intended for Islet Isolation: A Large-Scale, Single-Center Experience |
title_full_unstemmed |
Histidine-Tryptophan-Ketoglutarate and University of Wisconsin Solution Demonstrate Equal Effectiveness in the Preservation of Human Pancreata Intended for Islet Isolation: A Large-Scale, Single-Center Experience |
title_sort |
histidine-tryptophan-ketoglutarate and university of wisconsin solution demonstrate equal effectiveness in the preservation of human pancreata intended for islet isolation: a large-scale, single-center experience |
publisher |
SAGE Publishing |
series |
Cell Transplantation |
issn |
0963-6897 1555-3892 |
publishDate |
2013-07-01 |
description |
We previously reported a small-scale study on the efficacy of histidine-tryptophan-ketoglutarate (HTK) solution versus University of Wisconsin (UW) solution on pancreas preservation for islet isolation. In this large-scale, retrospective analysis ( n = 252), we extend our initial description of the impact of HTK on islet isolation outcomes and include pancreatic digestion efficacy, purification outcomes, and islet size distribution. Multivariable linear regression analysis, adjusted for donor age, sex, BMI, cold ischemia time, and enzyme, demonstrated similar results for the HTK group ( n = 95) and the UW group ( n = 157), including postpurification islet yields (HTK: 289,702 IEQ vs. UW: 283,036 IEQ; p = 0.76), percentage of digested pancreatic tissue (HTK: 66.9% vs. UW: 64.1%; p = 0.18), and islet loss from postdigestion to postpurification (HTK: 24,972 IEQ vs. UW: 39,551 IEQ; p = 0.38). Changes in islet size between the postdigestion and postpurification stages were comparable within each islet size category for HTK and UW ( p = 0.14–0.99). Tissue volume distribution across purification fractions and islet purity in the top fractions were similar between the groups; however, the HTK group had significantly higher islet purity in the middle fractions ( p = 0.003–0.008). Islet viability and stimulation indices were also similar between the HTK and the UW groups. In addition, we analyzed a small sample of patients transplanted either with HTK ( n = 7) or UW ( n = 8) preserved islets and found no significant differences in posttransplant HbA 1c , β-score, and frequency of insulin independence. This study demonstrates that HTK and UW solutions offer comparable pancreas preservation for islet transplantation. More in vivo islet outcome data are needed for a complete analysis of the effects of HTK on islet transplantation. |
url |
https://doi.org/10.3727/096368912X657332 |
work_keys_str_mv |
AT danielhpaushter histidinetryptophanketoglutarateanduniversityofwisconsinsolutiondemonstrateequaleffectivenessinthepreservationofhumanpancreataintendedforisletisolationalargescalesinglecenterexperience AT meirigengqi histidinetryptophanketoglutarateanduniversityofwisconsinsolutiondemonstrateequaleffectivenessinthepreservationofhumanpancreataintendedforisletisolationalargescalesinglecenterexperience AT kirstiekdanielson histidinetryptophanketoglutarateanduniversityofwisconsinsolutiondemonstrateequaleffectivenessinthepreservationofhumanpancreataintendedforisletisolationalargescalesinglecenterexperience AT triciaaharvat histidinetryptophanketoglutarateanduniversityofwisconsinsolutiondemonstrateequaleffectivenessinthepreservationofhumanpancreataintendedforisletisolationalargescalesinglecenterexperience AT katiekinzer histidinetryptophanketoglutarateanduniversityofwisconsinsolutiondemonstrateequaleffectivenessinthepreservationofhumanpancreataintendedforisletisolationalargescalesinglecenterexperience AT barbarabarbaro histidinetryptophanketoglutarateanduniversityofwisconsinsolutiondemonstrateequaleffectivenessinthepreservationofhumanpancreataintendedforisletisolationalargescalesinglecenterexperience AT sonnypatel histidinetryptophanketoglutarateanduniversityofwisconsinsolutiondemonstrateequaleffectivenessinthepreservationofhumanpancreataintendedforisletisolationalargescalesinglecenterexperience AT sarahzhassan histidinetryptophanketoglutarateanduniversityofwisconsinsolutiondemonstrateequaleffectivenessinthepreservationofhumanpancreataintendedforisletisolationalargescalesinglecenterexperience AT joseoberholzer histidinetryptophanketoglutarateanduniversityofwisconsinsolutiondemonstrateequaleffectivenessinthepreservationofhumanpancreataintendedforisletisolationalargescalesinglecenterexperience AT yongwang histidinetryptophanketoglutarateanduniversityofwisconsinsolutiondemonstrateequaleffectivenessinthepreservationofhumanpancreataintendedforisletisolationalargescalesinglecenterexperience |
_version_ |
1724687790407417856 |