Screening of Korean Natural Products for Anti-Adipogenesis Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from Solidago virgaurea
In this study, the anti-adipogenetic activity of 300 plant extracts was investigated using an Oil Red O staining assay in a 3T3-L1 cell line. Our results indicate that three plants, including the stem and leaf of Physalis angulata, the whole grass of Solidago virgaurea, and the root of Dioscorea nip...
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doaj-be6b4f42f0fa40bc8f16d7f234aa77ba2020-11-24T23:46:15ZengMDPI AGMolecules1420-30492016-02-0121222610.3390/molecules21020226molecules21020226Screening of Korean Natural Products for Anti-Adipogenesis Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from Solidago virgaureaYoung Soo Jang0Zhiqiang Wang1Jeong-Min Lee2Jae-Young Lee3Soon Sung Lim4Department of Food Science and Nutrition, Hallym University, Okchon-dong, Chuncheon, Gangwon-do 200-702, KoreaDepartment of Food Science and Nutrition, Hallym University, Okchon-dong, Chuncheon, Gangwon-do 200-702, KoreaDepartment of Boichemistry, Hallym University Medical School, Okchon-dong, Chuncheon, Gangwon-do 200-702, KoreaDepartment of Boichemistry, Hallym University Medical School, Okchon-dong, Chuncheon, Gangwon-do 200-702, KoreaDepartment of Food Science and Nutrition, Hallym University, Okchon-dong, Chuncheon, Gangwon-do 200-702, KoreaIn this study, the anti-adipogenetic activity of 300 plant extracts was investigated using an Oil Red O staining assay in a 3T3-L1 cell line. Our results indicate that three plants, including the stem and leaf of Physalis angulata, the whole grass of Solidago virgaurea, and the root of Dioscorea nipponica, produced over 90% inhibition of adipogenesis. Kaempferol-3-O-rutinoside, which demonstrated a 48.2% inhibitory effect on adipogenesis without cytotoxicity, was isolated from the butanol layer of a water extract of S. virgaurea guided by the anti-adipogenesis assay in 3T3-L1. PPAR-γ and C/EBPα expression levels were determined using western blot, and our results indicate that kaempferol-3-O-rutinoside has a strong anti-adipogenic effect in 3T3-L1 cells through the suppression of increases in PPAR-γ and C/EBPα expression.http://www.mdpi.com/1420-3049/21/2/226obesityscreeningSolidago virgaureakaempferol-3-O-rutinoside |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Young Soo Jang Zhiqiang Wang Jeong-Min Lee Jae-Young Lee Soon Sung Lim |
spellingShingle |
Young Soo Jang Zhiqiang Wang Jeong-Min Lee Jae-Young Lee Soon Sung Lim Screening of Korean Natural Products for Anti-Adipogenesis Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from Solidago virgaurea Molecules obesity screening Solidago virgaurea kaempferol-3-O-rutinoside |
author_facet |
Young Soo Jang Zhiqiang Wang Jeong-Min Lee Jae-Young Lee Soon Sung Lim |
author_sort |
Young Soo Jang |
title |
Screening of Korean Natural Products for Anti-Adipogenesis Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from Solidago virgaurea |
title_short |
Screening of Korean Natural Products for Anti-Adipogenesis Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from Solidago virgaurea |
title_full |
Screening of Korean Natural Products for Anti-Adipogenesis Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from Solidago virgaurea |
title_fullStr |
Screening of Korean Natural Products for Anti-Adipogenesis Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from Solidago virgaurea |
title_full_unstemmed |
Screening of Korean Natural Products for Anti-Adipogenesis Properties and Isolation of Kaempferol-3-O-rutinoside as a Potent Anti-Adipogenetic Compound from Solidago virgaurea |
title_sort |
screening of korean natural products for anti-adipogenesis properties and isolation of kaempferol-3-o-rutinoside as a potent anti-adipogenetic compound from solidago virgaurea |
publisher |
MDPI AG |
series |
Molecules |
issn |
1420-3049 |
publishDate |
2016-02-01 |
description |
In this study, the anti-adipogenetic activity of 300 plant extracts was investigated using an Oil Red O staining assay in a 3T3-L1 cell line. Our results indicate that three plants, including the stem and leaf of Physalis angulata, the whole grass of Solidago virgaurea, and the root of Dioscorea nipponica, produced over 90% inhibition of adipogenesis. Kaempferol-3-O-rutinoside, which demonstrated a 48.2% inhibitory effect on adipogenesis without cytotoxicity, was isolated from the butanol layer of a water extract of S. virgaurea guided by the anti-adipogenesis assay in 3T3-L1. PPAR-γ and C/EBPα expression levels were determined using western blot, and our results indicate that kaempferol-3-O-rutinoside has a strong anti-adipogenic effect in 3T3-L1 cells through the suppression of increases in PPAR-γ and C/EBPα expression. |
topic |
obesity screening Solidago virgaurea kaempferol-3-O-rutinoside |
url |
http://www.mdpi.com/1420-3049/21/2/226 |
work_keys_str_mv |
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