MeCP2-mediated epigenetic regulation in senescent endothelial progenitor cells
Abstract Background Cellular aging may be associated with epigenetics. Methyl-CpG-binding protein 2 (MeCP2) and sirtuin 1 (SIRT1) are two important epigenetic factors. Our former work demonstrated that MeCP2 expression increased and SIRT1 expression decreased in senescent endothelial progenitor cell...
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doaj-becd8b27f8754ee8881b7d6aede43e4d2020-11-25T02:22:11ZengBMCStem Cell Research & Therapy1757-65122018-04-01911710.1186/s13287-018-0828-yMeCP2-mediated epigenetic regulation in senescent endothelial progenitor cellsChunli Wang0Fei Wang1Zhen Li2Qing Cao3Liya Huang4Shuyan Chen5Department of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineDepartment of Geriatrics, Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of MedicineAbstract Background Cellular aging may be associated with epigenetics. Methyl-CpG-binding protein 2 (MeCP2) and sirtuin 1 (SIRT1) are two important epigenetic factors. Our former work demonstrated that MeCP2 expression increased and SIRT1 expression decreased in senescent endothelial progenitor cells (EPCs). This article aims to reveal the epigenetic regulation caused by MeCP2 in EPCs and discuss its mechanism. Methods Tube formation assay and cell apoptosis detection were used to evaluate the function of senescent EPCs induced by MeCP2 overexpression. Western blot analysis was used to testify the relative protein expression changed by MeCP2. Bisulfite sequencing methylation assay and chromatin immunoprecipitation assay were used to assess the degree of methylation and the relation of MeCP2 and SIRT1. Results MeCP2 reduced angiogenesis of senescent EPCs, promoted apoptosis, and caused senescent EPC dysfunction through SIRT1 promoter hypermethylation and histone modification. Conclusions MeCP2 mediated senescent EPC dysfunction through epigenetic regulation.http://link.springer.com/article/10.1186/s13287-018-0828-yEndothelial progenitor cellsSenescenceMeCP2SIRT1Epigenetic |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chunli Wang Fei Wang Zhen Li Qing Cao Liya Huang Shuyan Chen |
spellingShingle |
Chunli Wang Fei Wang Zhen Li Qing Cao Liya Huang Shuyan Chen MeCP2-mediated epigenetic regulation in senescent endothelial progenitor cells Stem Cell Research & Therapy Endothelial progenitor cells Senescence MeCP2 SIRT1 Epigenetic |
author_facet |
Chunli Wang Fei Wang Zhen Li Qing Cao Liya Huang Shuyan Chen |
author_sort |
Chunli Wang |
title |
MeCP2-mediated epigenetic regulation in senescent endothelial progenitor cells |
title_short |
MeCP2-mediated epigenetic regulation in senescent endothelial progenitor cells |
title_full |
MeCP2-mediated epigenetic regulation in senescent endothelial progenitor cells |
title_fullStr |
MeCP2-mediated epigenetic regulation in senescent endothelial progenitor cells |
title_full_unstemmed |
MeCP2-mediated epigenetic regulation in senescent endothelial progenitor cells |
title_sort |
mecp2-mediated epigenetic regulation in senescent endothelial progenitor cells |
publisher |
BMC |
series |
Stem Cell Research & Therapy |
issn |
1757-6512 |
publishDate |
2018-04-01 |
description |
Abstract Background Cellular aging may be associated with epigenetics. Methyl-CpG-binding protein 2 (MeCP2) and sirtuin 1 (SIRT1) are two important epigenetic factors. Our former work demonstrated that MeCP2 expression increased and SIRT1 expression decreased in senescent endothelial progenitor cells (EPCs). This article aims to reveal the epigenetic regulation caused by MeCP2 in EPCs and discuss its mechanism. Methods Tube formation assay and cell apoptosis detection were used to evaluate the function of senescent EPCs induced by MeCP2 overexpression. Western blot analysis was used to testify the relative protein expression changed by MeCP2. Bisulfite sequencing methylation assay and chromatin immunoprecipitation assay were used to assess the degree of methylation and the relation of MeCP2 and SIRT1. Results MeCP2 reduced angiogenesis of senescent EPCs, promoted apoptosis, and caused senescent EPC dysfunction through SIRT1 promoter hypermethylation and histone modification. Conclusions MeCP2 mediated senescent EPC dysfunction through epigenetic regulation. |
topic |
Endothelial progenitor cells Senescence MeCP2 SIRT1 Epigenetic |
url |
http://link.springer.com/article/10.1186/s13287-018-0828-y |
work_keys_str_mv |
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