DA-9801 and its saponins, dioscin and protodioscin, protect primary cortical neurons from hyperglycemia-induced neurotoxicity
Neurodegenerative diseases, including Alzheimer’s disease, are prevalent in diabetic patients. DA-9801 is a standardized extract of Dioscorea japonica Thunb. and Dioscorea nipponica Makino, which has been shown to reduce blood glucose levels and exert protective effects against diabetic peripheral n...
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doaj-bed234cfc8684120abb94c483b656e4a2021-04-30T07:15:35ZengElsevierJournal of Functional Foods1756-46462019-03-0154231240DA-9801 and its saponins, dioscin and protodioscin, protect primary cortical neurons from hyperglycemia-induced neurotoxicityHa-Rim Lee0Sun-Young Kong1Sang Hyun Sung2Hyun-Jung Kim3Laboratory of Molecular and Stem Cell Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of KoreaLaboratory of Molecular and Stem Cell Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of KoreaCollege of Pharmacy and Research Institute of Pharmaceutical Science, Seoul National University, Seoul 08826, Republic of KoreaLaboratory of Molecular and Stem Cell Pharmacology, College of Pharmacy, Chung-Ang University, Seoul 156-756, Republic of Korea; Corresponding author.Neurodegenerative diseases, including Alzheimer’s disease, are prevalent in diabetic patients. DA-9801 is a standardized extract of Dioscorea japonica Thunb. and Dioscorea nipponica Makino, which has been shown to reduce blood glucose levels and exert protective effects against diabetic peripheral neuropathy. Here, we aimed to investigate whether DA-9801 could protect hyperglycemia-induced central neuropathy and to elucidate the underlying mechanisms. DA-9801 protected rat cortical primary neurons against hyperglycemia-induced neurotoxicity by lowering the levels of reactive oxygen species. In addition, we identified the genes whose expression in high glucose-treated primary neurons was significantly altered by DA-9801 treatment. Moreover, the major saponins of DA-9801, dioscin and protodioscin, protected neurons from hyperglycemia-induced toxicity, suggesting that these may be the most important active neuroprotective components of DA-9801. Our results showed that DA-9801 protects neurons derived from the central nervous system and that it may be useful to treat and delay the development of neurodegenerative diseases in diabetic patients.http://www.sciencedirect.com/science/article/pii/S1756464619300222AntioxidantDA-9801Gene-expressionMicroarrayNeuroprotectionPrimary neurons |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ha-Rim Lee Sun-Young Kong Sang Hyun Sung Hyun-Jung Kim |
spellingShingle |
Ha-Rim Lee Sun-Young Kong Sang Hyun Sung Hyun-Jung Kim DA-9801 and its saponins, dioscin and protodioscin, protect primary cortical neurons from hyperglycemia-induced neurotoxicity Journal of Functional Foods Antioxidant DA-9801 Gene-expression Microarray Neuroprotection Primary neurons |
author_facet |
Ha-Rim Lee Sun-Young Kong Sang Hyun Sung Hyun-Jung Kim |
author_sort |
Ha-Rim Lee |
title |
DA-9801 and its saponins, dioscin and protodioscin, protect primary cortical neurons from hyperglycemia-induced neurotoxicity |
title_short |
DA-9801 and its saponins, dioscin and protodioscin, protect primary cortical neurons from hyperglycemia-induced neurotoxicity |
title_full |
DA-9801 and its saponins, dioscin and protodioscin, protect primary cortical neurons from hyperglycemia-induced neurotoxicity |
title_fullStr |
DA-9801 and its saponins, dioscin and protodioscin, protect primary cortical neurons from hyperglycemia-induced neurotoxicity |
title_full_unstemmed |
DA-9801 and its saponins, dioscin and protodioscin, protect primary cortical neurons from hyperglycemia-induced neurotoxicity |
title_sort |
da-9801 and its saponins, dioscin and protodioscin, protect primary cortical neurons from hyperglycemia-induced neurotoxicity |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2019-03-01 |
description |
Neurodegenerative diseases, including Alzheimer’s disease, are prevalent in diabetic patients. DA-9801 is a standardized extract of Dioscorea japonica Thunb. and Dioscorea nipponica Makino, which has been shown to reduce blood glucose levels and exert protective effects against diabetic peripheral neuropathy. Here, we aimed to investigate whether DA-9801 could protect hyperglycemia-induced central neuropathy and to elucidate the underlying mechanisms. DA-9801 protected rat cortical primary neurons against hyperglycemia-induced neurotoxicity by lowering the levels of reactive oxygen species. In addition, we identified the genes whose expression in high glucose-treated primary neurons was significantly altered by DA-9801 treatment. Moreover, the major saponins of DA-9801, dioscin and protodioscin, protected neurons from hyperglycemia-induced toxicity, suggesting that these may be the most important active neuroprotective components of DA-9801. Our results showed that DA-9801 protects neurons derived from the central nervous system and that it may be useful to treat and delay the development of neurodegenerative diseases in diabetic patients. |
topic |
Antioxidant DA-9801 Gene-expression Microarray Neuroprotection Primary neurons |
url |
http://www.sciencedirect.com/science/article/pii/S1756464619300222 |
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