Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles
Nine types of solid lipid nanoparticle (SLN) formulations were produced using tripalmitin (TPM), glyceryl monostearate (GM) or stearic acid (SA), stabilized with lecithin S75 and polysorbate 80. Formulations were prepared presenting PI values within 0.25 to 0.30, and the physicochemical properties,...
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doaj-c19c605d49f044bcb29eeb87ca95a0612020-11-24T23:22:21ZengMDPI AGInternational Journal of Environmental Research and Public Health1660-46012014-08-011188581859610.3390/ijerph110808581ijerph110808581Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid NanoparticlesCarine Dal Pizzol0Fabíola Branco Filippin-Monteiro1Jelver Alexander Sierra Restrepo2Frederico Pittella3Adny Henrique Silva4Paula Alves de Souza5Angela Machado de Campos6Tânia Beatriz Creczynski-Pasa7Departamento de Ciências Farmacêuticas, Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, BrazilDepartamento de Ciências Farmacêuticas, Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, BrazilProgama de Pós-Graduação em Engenharia de Materiais, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, BrazilDepartamento de Análises Clínicas, Universidade Federal de Juiz de Fora, Juiz de Fora, MG 36036-900, BrazilDepartamento de Ciências Farmacêuticas, Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, BrazilDepartamento de Ciências Farmacêuticas, Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, BrazilDepartamento de Ciências Farmacêuticas, Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, BrazilDepartamento de Ciências Farmacêuticas, Programa de Pós-Graduação em Farmácia, Universidade Federal de Santa Catarina, Florianópolis, SC 88040-900, BrazilNine types of solid lipid nanoparticle (SLN) formulations were produced using tripalmitin (TPM), glyceryl monostearate (GM) or stearic acid (SA), stabilized with lecithin S75 and polysorbate 80. Formulations were prepared presenting PI values within 0.25 to 0.30, and the physicochemical properties, stability upon storage and biocompatibility were evaluated. The average particle size ranged from 116 to 306 nm, with a negative surface charge around −11 mV. SLN presented good stability up to 60 days. The SLN manufactured using SA could not be measured by DLS due to the reflective feature of this formulation. However, TEM images revealed that SA nanoparticles presented square/rod shapes with an approximate size of 100 nm. Regarding biocompatibility aspects, SA nanoparticles showed toxicity in fibroblasts, causing cell death, and produced high hemolytic rates, indicating toxicity to red blood cells. This finding might be related to lipid type, as well as, the shape of the nanoparticles. No morphological alterations and hemolytic effects were observed in cells incubated with SLN containing TPM and GM. The SLN containing TPM and GM showed long-term stability, suggesting good shelf-life. The results indicate high toxicity of SLN prepared with SA, and strongly suggest that the components of the formulation should be analyzed in combination rather than separately to avoid misinterpretation of the results.http://www.mdpi.com/1660-4601/11/8/8581solid lipid nanoparticlescytotoxicitysurfactantlipidbiocompatibility |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Carine Dal Pizzol Fabíola Branco Filippin-Monteiro Jelver Alexander Sierra Restrepo Frederico Pittella Adny Henrique Silva Paula Alves de Souza Angela Machado de Campos Tânia Beatriz Creczynski-Pasa |
spellingShingle |
Carine Dal Pizzol Fabíola Branco Filippin-Monteiro Jelver Alexander Sierra Restrepo Frederico Pittella Adny Henrique Silva Paula Alves de Souza Angela Machado de Campos Tânia Beatriz Creczynski-Pasa Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles International Journal of Environmental Research and Public Health solid lipid nanoparticles cytotoxicity surfactant lipid biocompatibility |
author_facet |
Carine Dal Pizzol Fabíola Branco Filippin-Monteiro Jelver Alexander Sierra Restrepo Frederico Pittella Adny Henrique Silva Paula Alves de Souza Angela Machado de Campos Tânia Beatriz Creczynski-Pasa |
author_sort |
Carine Dal Pizzol |
title |
Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles |
title_short |
Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles |
title_full |
Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles |
title_fullStr |
Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles |
title_full_unstemmed |
Influence of Surfactant and Lipid Type on the Physicochemical Properties and Biocompatibility of Solid Lipid Nanoparticles |
title_sort |
influence of surfactant and lipid type on the physicochemical properties and biocompatibility of solid lipid nanoparticles |
publisher |
MDPI AG |
series |
International Journal of Environmental Research and Public Health |
issn |
1660-4601 |
publishDate |
2014-08-01 |
description |
Nine types of solid lipid nanoparticle (SLN) formulations were produced using tripalmitin (TPM), glyceryl monostearate (GM) or stearic acid (SA), stabilized with lecithin S75 and polysorbate 80. Formulations were prepared presenting PI values within 0.25 to 0.30, and the physicochemical properties, stability upon storage and biocompatibility were evaluated. The average particle size ranged from 116 to 306 nm, with a negative surface charge around −11 mV. SLN presented good stability up to 60 days. The SLN manufactured using SA could not be measured by DLS due to the reflective feature of this formulation. However, TEM images revealed that SA nanoparticles presented square/rod shapes with an approximate size of 100 nm. Regarding biocompatibility aspects, SA nanoparticles showed toxicity in fibroblasts, causing cell death, and produced high hemolytic rates, indicating toxicity to red blood cells. This finding might be related to lipid type, as well as, the shape of the nanoparticles. No morphological alterations and hemolytic effects were observed in cells incubated with SLN containing TPM and GM. The SLN containing TPM and GM showed long-term stability, suggesting good shelf-life. The results indicate high toxicity of SLN prepared with SA, and strongly suggest that the components of the formulation should be analyzed in combination rather than separately to avoid misinterpretation of the results. |
topic |
solid lipid nanoparticles cytotoxicity surfactant lipid biocompatibility |
url |
http://www.mdpi.com/1660-4601/11/8/8581 |
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