Callus Induction and Rhizogenesis in Lathyrus sativus L.
Grass pea (Lathyrus sativus L.) is a leguminous plant distinguished by great resistance to abiotic and biotic stresses that can become a valuable source of protein feed in the nearest future. However, neglected by breeders, it needs the improvement of some disadvantages. Biotechnological techniques,...
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doaj-c2ce40185c78497c954a552de60fd0c92020-11-24T22:48:15ZengMendel University PressActa Universitatis Agriculturae et Silviculturae Mendelianae Brunensis1211-85162464-83102016-01-0164112312810.11118/actaun201664010123Callus Induction and Rhizogenesis in Lathyrus sativus L.Barbara Piwowarczyk0Anna Pindel1Ewa Muszyńska2Institute of Plant Biology and Biotechnology, Faculty of Biotechnology and Holticulture, University of Agriculture in Krakow, Al. 29-Listopada 54, 31-425 Krakow, PolandInstitute of Plant Biology and Biotechnology, Faculty of Biotechnology and Holticulture, University of Agriculture in Krakow, Al. 29-Listopada 54, 31-425 Krakow, PolandInstitute of Plant Biology and Biotechnology, Faculty of Biotechnology and Holticulture, University of Agriculture in Krakow, Al. 29-Listopada 54, 31-425 Krakow, PolandGrass pea (Lathyrus sativus L.) is a leguminous plant distinguished by great resistance to abiotic and biotic stresses that can become a valuable source of protein feed in the nearest future. However, neglected by breeders, it needs the improvement of some disadvantages. Biotechnological techniques, including in vitro culture, are new and modern tools supporting plant breeding. Unfortunately, grass pea belongs to grain legumes that are well-known for their recalcitrance to in vitro manipulation and consequently plant regeneration. The aim of the study was the evaluation of different factors influence on callus induction and proliferation, as well as cell differentiation and organogenesis. Callus culture were initiated from different explants of two Polish cultivars of grasspea on media supplemented with auxin and various cytokinins both on the light and in the dark. The rate of tissue proliferation was significantly increased in both tested cultivars by light. More than 400 mg of tissue from one explant was obtained after 12 weeks of culture. The most intensive increase in callus mass was noted for internode fragments of ‘Krab’ (380 mg/one explant) and root fragments of ‘Derek’ (361 mg/one explant). On the media with the addition of thidiazuron callus tissue grew better (852 mg/one explants) than on the media with zeatin and kinetin (56–164 mg/one explant). Formation of the roots was the only type of organogenesis observed during the study. In ‘Derek’ callus on LP medium rhizogenesis occurred the most frequently (38%). The roots regenerated from this callus was also the most numerous (1.6) and the longest (12.5 mm).https://acta.mendelu.cz/64/1/0123/callus tissuecytokininsgrass pealight conditionsroots type of explants |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Barbara Piwowarczyk Anna Pindel Ewa Muszyńska |
spellingShingle |
Barbara Piwowarczyk Anna Pindel Ewa Muszyńska Callus Induction and Rhizogenesis in Lathyrus sativus L. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis callus tissue cytokinins grass pea light conditions roots type of explants |
author_facet |
Barbara Piwowarczyk Anna Pindel Ewa Muszyńska |
author_sort |
Barbara Piwowarczyk |
title |
Callus Induction and Rhizogenesis in Lathyrus sativus L. |
title_short |
Callus Induction and Rhizogenesis in Lathyrus sativus L. |
title_full |
Callus Induction and Rhizogenesis in Lathyrus sativus L. |
title_fullStr |
Callus Induction and Rhizogenesis in Lathyrus sativus L. |
title_full_unstemmed |
Callus Induction and Rhizogenesis in Lathyrus sativus L. |
title_sort |
callus induction and rhizogenesis in lathyrus sativus l. |
publisher |
Mendel University Press |
series |
Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis |
issn |
1211-8516 2464-8310 |
publishDate |
2016-01-01 |
description |
Grass pea (Lathyrus sativus L.) is a leguminous plant distinguished by great resistance to abiotic and biotic stresses that can become a valuable source of protein feed in the nearest future. However, neglected by breeders, it needs the improvement of some disadvantages. Biotechnological techniques, including in vitro culture, are new and modern tools supporting plant breeding. Unfortunately, grass pea belongs to grain legumes that are well-known for their recalcitrance to in vitro manipulation and consequently plant regeneration. The aim of the study was the evaluation of different factors influence on callus induction and proliferation, as well as cell differentiation and organogenesis. Callus culture were initiated from different explants of two Polish cultivars of grasspea on media supplemented with auxin and various cytokinins both on the light and in the dark. The rate of tissue proliferation was significantly increased in both tested cultivars by light. More than 400 mg of tissue from one explant was obtained after 12 weeks of culture. The most intensive increase in callus mass was noted for internode fragments of ‘Krab’ (380 mg/one explant) and root fragments of ‘Derek’ (361 mg/one explant). On the media with the addition of thidiazuron callus tissue grew better (852 mg/one explants) than on the media with zeatin and kinetin (56–164 mg/one explant). Formation of the roots was the only type of organogenesis observed during the study. In ‘Derek’ callus on LP medium rhizogenesis occurred the most frequently (38%). The roots regenerated from this callus was also the most numerous (1.6) and the longest (12.5 mm). |
topic |
callus tissue cytokinins grass pea light conditions roots type of explants |
url |
https://acta.mendelu.cz/64/1/0123/ |
work_keys_str_mv |
AT barbarapiwowarczyk callusinductionandrhizogenesisinlathyrussativusl AT annapindel callusinductionandrhizogenesisinlathyrussativusl AT ewamuszynska callusinductionandrhizogenesisinlathyrussativusl |
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