Pseudocowpox virus infection in an American bison (Bison bison)
Abstract Background The present report describes a case of pseudocowpox virus (PCPV) infection in a seven-year-old female bison euthanized due to a history of declining condition and sores on the vulva and udder. Case presentation External examination revealed multifocal, raised, keratinized plaques...
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doaj-c3825fed4a5f4a1aaca80141f4782d0e2020-11-25T03:43:05ZengBMCBMC Veterinary Research1746-61482020-07-011611610.1186/s12917-020-02464-7Pseudocowpox virus infection in an American bison (Bison bison)Vinay Shivanna0A. Giselle Cino-Ozuna1Cody Heskett2Douglas G. Marthaler3Charan Ganta4Kansas State University Veterinary Diagnostic Laboratory, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityKansas State University Veterinary Diagnostic Laboratory, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityKansas State University Veterinary Diagnostic Laboratory, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityKansas State University Veterinary Diagnostic Laboratory, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityKansas State University Veterinary Diagnostic Laboratory, Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State UniversityAbstract Background The present report describes a case of pseudocowpox virus (PCPV) infection in a seven-year-old female bison euthanized due to a history of declining condition and sores on the vulva and udder. Case presentation External examination revealed multifocal, raised, keratinized plaques (0.5–2 cm) covering the skin of the ventral surface of the tail, perineum, caudoventral abdomen, udder, both inguinal recesses, and the medial aspects of both thighs. No significant gross lesions were present in the reminder of the tissues examined. Histopathological examination of the affected skin showed moderate epidermal hyperplasia with rete pegs, marked parakeratotic hyperkeratosis with crusts of degenerate neutrophils and cell debris, and few epithelial cells undergoing ballooning degeneration with occasional eosinophilic intracytoplasmic inclusion bodies (3–5 μm Bollinger body). Negative staining electron microscopy from skin revealed typical Parapoxvirus (PPV) particles, which were also confirmed by real-time PCR (Ct =18.6). Metagenomic analysis of the skin samples revealed only poxviruses. The bison parapox B2L envelope gene clustered with other parapox sequences identified from ruminants. Conclusions This is the first report of PCPV virus infection in an American bison. Identification of novel susceptible hosts of parapox viruses sheds light on the viral evolution and highlights the importance of potential economic impact of this disease to the bison industry.http://link.springer.com/article/10.1186/s12917-020-02464-7Pseudocowpox virusBisonZoonoticElectron microscopyInclusionsDeep-sequencing |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vinay Shivanna A. Giselle Cino-Ozuna Cody Heskett Douglas G. Marthaler Charan Ganta |
spellingShingle |
Vinay Shivanna A. Giselle Cino-Ozuna Cody Heskett Douglas G. Marthaler Charan Ganta Pseudocowpox virus infection in an American bison (Bison bison) BMC Veterinary Research Pseudocowpox virus Bison Zoonotic Electron microscopy Inclusions Deep-sequencing |
author_facet |
Vinay Shivanna A. Giselle Cino-Ozuna Cody Heskett Douglas G. Marthaler Charan Ganta |
author_sort |
Vinay Shivanna |
title |
Pseudocowpox virus infection in an American bison (Bison bison) |
title_short |
Pseudocowpox virus infection in an American bison (Bison bison) |
title_full |
Pseudocowpox virus infection in an American bison (Bison bison) |
title_fullStr |
Pseudocowpox virus infection in an American bison (Bison bison) |
title_full_unstemmed |
Pseudocowpox virus infection in an American bison (Bison bison) |
title_sort |
pseudocowpox virus infection in an american bison (bison bison) |
publisher |
BMC |
series |
BMC Veterinary Research |
issn |
1746-6148 |
publishDate |
2020-07-01 |
description |
Abstract Background The present report describes a case of pseudocowpox virus (PCPV) infection in a seven-year-old female bison euthanized due to a history of declining condition and sores on the vulva and udder. Case presentation External examination revealed multifocal, raised, keratinized plaques (0.5–2 cm) covering the skin of the ventral surface of the tail, perineum, caudoventral abdomen, udder, both inguinal recesses, and the medial aspects of both thighs. No significant gross lesions were present in the reminder of the tissues examined. Histopathological examination of the affected skin showed moderate epidermal hyperplasia with rete pegs, marked parakeratotic hyperkeratosis with crusts of degenerate neutrophils and cell debris, and few epithelial cells undergoing ballooning degeneration with occasional eosinophilic intracytoplasmic inclusion bodies (3–5 μm Bollinger body). Negative staining electron microscopy from skin revealed typical Parapoxvirus (PPV) particles, which were also confirmed by real-time PCR (Ct =18.6). Metagenomic analysis of the skin samples revealed only poxviruses. The bison parapox B2L envelope gene clustered with other parapox sequences identified from ruminants. Conclusions This is the first report of PCPV virus infection in an American bison. Identification of novel susceptible hosts of parapox viruses sheds light on the viral evolution and highlights the importance of potential economic impact of this disease to the bison industry. |
topic |
Pseudocowpox virus Bison Zoonotic Electron microscopy Inclusions Deep-sequencing |
url |
http://link.springer.com/article/10.1186/s12917-020-02464-7 |
work_keys_str_mv |
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