Staphylococcus aureus nasal carriage in hemodialysis centers of Fez, Morocco

• Main Authors: Idrissa Diawara, Khadija Bekhti, Driss Elhabchi, Rachid Saile, Naima Elmdaghri, Mohammed Timinouni, Mohamed Elazhari
• Format: Article
• Language: English
• Published: Tehran University of Medical Sciences 2014-06-01
• Series: Iranian Journal of Microbiology
• Subjects: Nasal carriage; Staphylococcus aureus; antimicrobial susceptibility; hemodialysis; toxin genes
• Online Access: https://ijm.tums.ac.ir/index.php/ijm/article/view/419

Background and objectives: Staphylococcus aureus (S. aureus) nasal carriage may be responsible for some serious infections in hemodialyzed patients. The main target of this study was to estimate the prevalence of S. aureus nasal carriage in hemodialysis outpatients and medical staff in hemodialysis centers specifically in Fez region. The second target is to identify the risks of colonization, resistance pattern of isolates and their virulence toxin genes. Patients and Methods: Nasal swab specimens were obtained from 143 hemodialyzed outpatients and 32 medical staff from January to June 2012. Each participant completed a short questionnaire. Nasal carriage of S. aureus was demographically related (age, gender, hemodialysis duration), comorbidity (diabetes, malignancy) and exposure to health care (dialysis staff, hospitalization). PCR were used on all the isolates in the research of twelve staphylococcal enterotoxins genes. Also, PCR was used to investigate on the three factors epidermal cell differentiation inhibitors; three exfoliatin toxins; two leukotoxins; the toxic shock syndrome toxin-1 and the hemolysin beta genes. Results: Nasal screening revealed 38.16%, 50% and 18.75% S. aureus carries in chronic, acute hemodialysis patients and medical staff, respectively. Only young participants were likely to be S. aureus carries (p = 0.002). There were no gender differences between the isolate carriers and non-carriers or some comorbidity factors such as viral hepatitis B and C, HIV infections, diabetes, chronic smoking, recent hospitalization or antibiotic therapy. Out of all isolates, only one (1.61%) was methicillin-resistant and Twenty-one (33.87%) had at least two virulence toxin genes. Conclusions: Knowledge and monitoring of antibiotic resistance profile and virulence of S. aureus carriage are essential in the treatment of infections generated by this pathogen, as well as in the control of clonal dissemination and prevent the spread of S. aureus resistance.