Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig

Rat and guinea pig liver microsomes labeled with phospholipid 32P were incubated with rat, guinea pig, and rabbit plasma in a KC1–Tris–EDTA buffer. A net transfer of microsomal phosphatidylcholine and phosphatidylethanolamine to plasma was observed. In addition, an exchange of phospholipids between...

Full description

Bibliographic Details
Main Author: D.B. ZILVERSMIT
Format: Article
Language:English
Published: Elsevier 1971-01-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520395444
id doaj-c7e8ed5060da4a9bb02b1bd86d6b280a
record_format Article
spelling doaj-c7e8ed5060da4a9bb02b1bd86d6b280a2021-04-24T05:52:15ZengElsevierJournal of Lipid Research0022-22751971-01-011213642Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pigD.B. ZILVERSMIT0Graduate School of Nutrition; and Section of Biochemistry and Molecular Biology, Division of Biological Sciences, Cornel1 University, Ithaca, New York 14850Rat and guinea pig liver microsomes labeled with phospholipid 32P were incubated with rat, guinea pig, and rabbit plasma in a KC1–Tris–EDTA buffer. A net transfer of microsomal phosphatidylcholine and phosphatidylethanolamine to plasma was observed. In addition, an exchange of phospholipids between microsomes and plasma took place. During 20-min incubations at 37°C, the exchange of phosphatidylcholine was the most extensive. Microsomal sphingomyelin exchanged with plasma sphingomyelin only very slowly. A soluble protein factor in liver, which had previously been observed to stimulate the exchange of liver mitochondrial and microsomal phospholipids, also increased the exchange of phosphatidylcholine between liver microsomes and plasma. The pronounced differences in the relative percentages of phosphatidylethanolamine of guinea pig, rabbit, and rat plasmas did not appear to be related to differences in the relative exchange of this phospholipid compared to that of other phospholipids in these plasmas.http://www.sciencedirect.com/science/article/pii/S0022227520395444phosphatidylcholinephosphatidylethanolaminephosphatidylinositollysophosphatidylcholinesphingomyelin
collection DOAJ
language English
format Article
sources DOAJ
author D.B. ZILVERSMIT
spellingShingle D.B. ZILVERSMIT
Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig
Journal of Lipid Research
phosphatidylcholine
phosphatidylethanolamine
phosphatidylinositol
lysophosphatidylcholine
sphingomyelin
author_facet D.B. ZILVERSMIT
author_sort D.B. ZILVERSMIT
title Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig
title_short Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig
title_full Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig
title_fullStr Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig
title_full_unstemmed Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig
title_sort exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig
publisher Elsevier
series Journal of Lipid Research
issn 0022-2275
publishDate 1971-01-01
description Rat and guinea pig liver microsomes labeled with phospholipid 32P were incubated with rat, guinea pig, and rabbit plasma in a KC1–Tris–EDTA buffer. A net transfer of microsomal phosphatidylcholine and phosphatidylethanolamine to plasma was observed. In addition, an exchange of phospholipids between microsomes and plasma took place. During 20-min incubations at 37°C, the exchange of phosphatidylcholine was the most extensive. Microsomal sphingomyelin exchanged with plasma sphingomyelin only very slowly. A soluble protein factor in liver, which had previously been observed to stimulate the exchange of liver mitochondrial and microsomal phospholipids, also increased the exchange of phosphatidylcholine between liver microsomes and plasma. The pronounced differences in the relative percentages of phosphatidylethanolamine of guinea pig, rabbit, and rat plasmas did not appear to be related to differences in the relative exchange of this phospholipid compared to that of other phospholipids in these plasmas.
topic phosphatidylcholine
phosphatidylethanolamine
phosphatidylinositol
lysophosphatidylcholine
sphingomyelin
url http://www.sciencedirect.com/science/article/pii/S0022227520395444
work_keys_str_mv AT dbzilversmit exchangeofphospholipidclassesbetweenlivermicrosomesandplasmacomparisonofratrabbitandguineapig
_version_ 1721511622290178048