Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig
Rat and guinea pig liver microsomes labeled with phospholipid 32P were incubated with rat, guinea pig, and rabbit plasma in a KC1–Tris–EDTA buffer. A net transfer of microsomal phosphatidylcholine and phosphatidylethanolamine to plasma was observed. In addition, an exchange of phospholipids between...
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1971-01-01
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520395444 |
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doaj-c7e8ed5060da4a9bb02b1bd86d6b280a2021-04-24T05:52:15ZengElsevierJournal of Lipid Research0022-22751971-01-011213642Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pigD.B. ZILVERSMIT0Graduate School of Nutrition; and Section of Biochemistry and Molecular Biology, Division of Biological Sciences, Cornel1 University, Ithaca, New York 14850Rat and guinea pig liver microsomes labeled with phospholipid 32P were incubated with rat, guinea pig, and rabbit plasma in a KC1–Tris–EDTA buffer. A net transfer of microsomal phosphatidylcholine and phosphatidylethanolamine to plasma was observed. In addition, an exchange of phospholipids between microsomes and plasma took place. During 20-min incubations at 37°C, the exchange of phosphatidylcholine was the most extensive. Microsomal sphingomyelin exchanged with plasma sphingomyelin only very slowly. A soluble protein factor in liver, which had previously been observed to stimulate the exchange of liver mitochondrial and microsomal phospholipids, also increased the exchange of phosphatidylcholine between liver microsomes and plasma. The pronounced differences in the relative percentages of phosphatidylethanolamine of guinea pig, rabbit, and rat plasmas did not appear to be related to differences in the relative exchange of this phospholipid compared to that of other phospholipids in these plasmas.http://www.sciencedirect.com/science/article/pii/S0022227520395444phosphatidylcholinephosphatidylethanolaminephosphatidylinositollysophosphatidylcholinesphingomyelin |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
D.B. ZILVERSMIT |
spellingShingle |
D.B. ZILVERSMIT Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig Journal of Lipid Research phosphatidylcholine phosphatidylethanolamine phosphatidylinositol lysophosphatidylcholine sphingomyelin |
author_facet |
D.B. ZILVERSMIT |
author_sort |
D.B. ZILVERSMIT |
title |
Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig |
title_short |
Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig |
title_full |
Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig |
title_fullStr |
Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig |
title_full_unstemmed |
Exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig |
title_sort |
exchange of phospholipid classes between liver microsomes and plasma: comparison of rat, rabbit, and guinea pig |
publisher |
Elsevier |
series |
Journal of Lipid Research |
issn |
0022-2275 |
publishDate |
1971-01-01 |
description |
Rat and guinea pig liver microsomes labeled with phospholipid 32P were incubated with rat, guinea pig, and rabbit plasma in a KC1–Tris–EDTA buffer. A net transfer of microsomal phosphatidylcholine and phosphatidylethanolamine to plasma was observed. In addition, an exchange of phospholipids between microsomes and plasma took place. During 20-min incubations at 37°C, the exchange of phosphatidylcholine was the most extensive. Microsomal sphingomyelin exchanged with plasma sphingomyelin only very slowly. A soluble protein factor in liver, which had previously been observed to stimulate the exchange of liver mitochondrial and microsomal phospholipids, also increased the exchange of phosphatidylcholine between liver microsomes and plasma. The pronounced differences in the relative percentages of phosphatidylethanolamine of guinea pig, rabbit, and rat plasmas did not appear to be related to differences in the relative exchange of this phospholipid compared to that of other phospholipids in these plasmas. |
topic |
phosphatidylcholine phosphatidylethanolamine phosphatidylinositol lysophosphatidylcholine sphingomyelin |
url |
http://www.sciencedirect.com/science/article/pii/S0022227520395444 |
work_keys_str_mv |
AT dbzilversmit exchangeofphospholipidclassesbetweenlivermicrosomesandplasmacomparisonofratrabbitandguineapig |
_version_ |
1721511622290178048 |