Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situ
OBJECTIVE: Intestinal ischemia-reperfusion injury occurs in several clinical conditions and after intestinal transplantation. The aim of the present study was to investigate the phenomena of apoptosis and cell proliferation in a previously described intestinal ischemia-reperfusion injury autograft m...
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2015-05-01
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doaj-ca6fc9697ec24d0fb70968fa5cf894452020-11-24T22:40:35ZengFaculdade de Medicina / USPClinics1980-53222015-05-0170537337910.6061/clinics/2015(05)12S1807-59322015000500373Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situMaria Mercês SantosAna Cristina Aoun TannuriMaria Cecilia Mendonça CoelhoJosiane de Oliveira GonçalvesSuellen SerafiniLuiz Fernando Ferraz da SilvaUenis TannuriOBJECTIVE: Intestinal ischemia-reperfusion injury occurs in several clinical conditions and after intestinal transplantation. The aim of the present study was to investigate the phenomena of apoptosis and cell proliferation in a previously described intestinal ischemia-reperfusion injury autograft model using immunohistochemical markers. The molecular mechanisms involved in ischemia-reperfusion injury repair were also investigated by measuring the expression of the early activation genes c-fos and c-jun, which induce apoptosis and cell proliferation. MATERIALS AND METHODS: Thirty adult male Wistar rats were subjected to surgery for a previously described ischemia-reperfusion model that preserved the small intestine, the cecum and the ascending colon. Following reperfusion, the cecum was harvested at different time points as a representative segment of the intestine. The rats were allocated to the following four subgroups according to the reperfusion time: subgroup 1: 5 min; subgroup 2: 15 min; subgroup 3: 30 min; and subgroup 4: 60 min. A control group of cecum samples was also collected. The expression of c-fos, c-jun and immunohistochemical markers of cell proliferation and apoptosis (Ki67 and TUNEL, respectively) was studied. RESULTS: The expression of both c-fos and c-jun in the cecum was increased beginning at 5 min after ischemia-reperfusion compared with the control. The expression of c-fos began to increase at 5 min, peaked at 30 min, and exhibited a declining tendency at 60 min after reperfusion. A progressive increase in c-jun expression was observed. Immunohistochemical analyses confirmed these observations. CONCLUSION: The early activation of the c-fos and c-jun genes occurred after intestinal ischemia-reperfusion injury, and these genes can act together to trigger cell proliferation and apoptosis.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322015000500373&lng=en&tlng=enIschemia-reperfusion injuryImmediate early genesActivator protein 1Small intestineC-fosC-jun gene |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Maria Mercês Santos Ana Cristina Aoun Tannuri Maria Cecilia Mendonça Coelho Josiane de Oliveira Gonçalves Suellen Serafini Luiz Fernando Ferraz da Silva Uenis Tannuri |
spellingShingle |
Maria Mercês Santos Ana Cristina Aoun Tannuri Maria Cecilia Mendonça Coelho Josiane de Oliveira Gonçalves Suellen Serafini Luiz Fernando Ferraz da Silva Uenis Tannuri Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situ Clinics Ischemia-reperfusion injury Immediate early genes Activator protein 1 Small intestine C-fos C-jun gene |
author_facet |
Maria Mercês Santos Ana Cristina Aoun Tannuri Maria Cecilia Mendonça Coelho Josiane de Oliveira Gonçalves Suellen Serafini Luiz Fernando Ferraz da Silva Uenis Tannuri |
author_sort |
Maria Mercês Santos |
title |
Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situ |
title_short |
Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situ |
title_full |
Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situ |
title_fullStr |
Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situ |
title_full_unstemmed |
Immediate expression of c-fos and c-jun mRNA in a model of intestinal autotransplantation and ischemia-reperfusion in situ |
title_sort |
immediate expression of c-fos and c-jun mrna in a model of intestinal autotransplantation and ischemia-reperfusion in situ |
publisher |
Faculdade de Medicina / USP |
series |
Clinics |
issn |
1980-5322 |
publishDate |
2015-05-01 |
description |
OBJECTIVE: Intestinal ischemia-reperfusion injury occurs in several clinical conditions and after intestinal transplantation. The aim of the present study was to investigate the phenomena of apoptosis and cell proliferation in a previously described intestinal ischemia-reperfusion injury autograft model using immunohistochemical markers. The molecular mechanisms involved in ischemia-reperfusion injury repair were also investigated by measuring the expression of the early activation genes c-fos and c-jun, which induce apoptosis and cell proliferation. MATERIALS AND METHODS: Thirty adult male Wistar rats were subjected to surgery for a previously described ischemia-reperfusion model that preserved the small intestine, the cecum and the ascending colon. Following reperfusion, the cecum was harvested at different time points as a representative segment of the intestine. The rats were allocated to the following four subgroups according to the reperfusion time: subgroup 1: 5 min; subgroup 2: 15 min; subgroup 3: 30 min; and subgroup 4: 60 min. A control group of cecum samples was also collected. The expression of c-fos, c-jun and immunohistochemical markers of cell proliferation and apoptosis (Ki67 and TUNEL, respectively) was studied. RESULTS: The expression of both c-fos and c-jun in the cecum was increased beginning at 5 min after ischemia-reperfusion compared with the control. The expression of c-fos began to increase at 5 min, peaked at 30 min, and exhibited a declining tendency at 60 min after reperfusion. A progressive increase in c-jun expression was observed. Immunohistochemical analyses confirmed these observations. CONCLUSION: The early activation of the c-fos and c-jun genes occurred after intestinal ischemia-reperfusion injury, and these genes can act together to trigger cell proliferation and apoptosis. |
topic |
Ischemia-reperfusion injury Immediate early genes Activator protein 1 Small intestine C-fos C-jun gene |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1807-59322015000500373&lng=en&tlng=en |
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