Identification of a novel triterpene saponin from Panax ginseng seeds, pseudoginsenoside RT8, and its antiinflammatory activity
Background: Panax ginseng Meyer (Araliaceae) is a highly valued medicinal plant in Asian regions, especially in Korea, China, and Japan. Chemical and biological studies on P. ginseng have focused primarily on its roots, whereas the seeds remain poorly understood. This study explores the phytochemica...
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doaj-cb475a86f14f4a41889e2279da683eb52020-11-25T01:33:52ZengElsevierJournal of Ginseng Research1226-84532020-01-01441145153Identification of a novel triterpene saponin from Panax ginseng seeds, pseudoginsenoside RT8, and its antiinflammatory activityTaewoong Rho0Hyun Woo Jeong1Yong Deog Hong2Keejung Yoon3Jae Youl Cho4Kee Dong Yoon5College of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon, Republic of KoreaAmorepacific Corp. R&D Unit, Gyeonggi, Republic of KoreaAmorepacific Corp. R&D Unit, Gyeonggi, Republic of KoreaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, Republic of KoreaDepartment of Integrative Biotechnology, Sungkyunkwan University, Suwon, Republic of KoreaCollege of Pharmacy and Integrated Research Institute of Pharmaceutical Sciences, The Catholic University of Korea, Bucheon, Republic of Korea; Corresponding author. College of Pharmacy, The Catholic University of Korea, 43 Jibong-ro, Wonmi-gu, Bucheon-si, Gyeonggi-do 14662, Republic of Korea.Background: Panax ginseng Meyer (Araliaceae) is a highly valued medicinal plant in Asian regions, especially in Korea, China, and Japan. Chemical and biological studies on P. ginseng have focused primarily on its roots, whereas the seeds remain poorly understood. This study explores the phytochemical and biological properties of compounds from P. ginseng seeds. Methods: P. ginseng seeds were extracted with methanol, and 16 compounds were isolated using various chromatographic methods. The chemical structures of the isolates were determined by spectroscopic data. Antiinflammatory activities were evaluated for triterpene and steroidal saponins using lipopolysaccharide-stimulated RAW264.7 macrophages and THP-1 monocyte leukemia cells. Results: Phytochemical investigation of P. ginseng seeds led to the isolation of a novel triterpene saponin, pseudoginsenoside RT8, along with 15 known compounds. Pseudoginsenoside RT8 exhibited more potent antiinflammatory activity than the other saponins, attenuating lipopolysaccharide-mediated induction of proinflammatory genes such as interleukin-1β, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2, and matrix metalloproteinase-9, and suppressed reactive oxygen species and nitric oxide generation in a dose-dependent manner. Conclusion: These findings indicate that pseudoginsenoside RT8 has a pharmaceutical potential as an antiinflammatory agent and that P. ginseng seeds are a good natural source for discovering novel bioactive molecules. Keywords: Antiinflammatory activity, Panax ginseng seeds, Pseudoginsenoside RT8http://www.sciencedirect.com/science/article/pii/S1226845318301003 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Taewoong Rho Hyun Woo Jeong Yong Deog Hong Keejung Yoon Jae Youl Cho Kee Dong Yoon |
spellingShingle |
Taewoong Rho Hyun Woo Jeong Yong Deog Hong Keejung Yoon Jae Youl Cho Kee Dong Yoon Identification of a novel triterpene saponin from Panax ginseng seeds, pseudoginsenoside RT8, and its antiinflammatory activity Journal of Ginseng Research |
author_facet |
Taewoong Rho Hyun Woo Jeong Yong Deog Hong Keejung Yoon Jae Youl Cho Kee Dong Yoon |
author_sort |
Taewoong Rho |
title |
Identification of a novel triterpene saponin from Panax ginseng seeds, pseudoginsenoside RT8, and its antiinflammatory activity |
title_short |
Identification of a novel triterpene saponin from Panax ginseng seeds, pseudoginsenoside RT8, and its antiinflammatory activity |
title_full |
Identification of a novel triterpene saponin from Panax ginseng seeds, pseudoginsenoside RT8, and its antiinflammatory activity |
title_fullStr |
Identification of a novel triterpene saponin from Panax ginseng seeds, pseudoginsenoside RT8, and its antiinflammatory activity |
title_full_unstemmed |
Identification of a novel triterpene saponin from Panax ginseng seeds, pseudoginsenoside RT8, and its antiinflammatory activity |
title_sort |
identification of a novel triterpene saponin from panax ginseng seeds, pseudoginsenoside rt8, and its antiinflammatory activity |
publisher |
Elsevier |
series |
Journal of Ginseng Research |
issn |
1226-8453 |
publishDate |
2020-01-01 |
description |
Background: Panax ginseng Meyer (Araliaceae) is a highly valued medicinal plant in Asian regions, especially in Korea, China, and Japan. Chemical and biological studies on P. ginseng have focused primarily on its roots, whereas the seeds remain poorly understood. This study explores the phytochemical and biological properties of compounds from P. ginseng seeds. Methods: P. ginseng seeds were extracted with methanol, and 16 compounds were isolated using various chromatographic methods. The chemical structures of the isolates were determined by spectroscopic data. Antiinflammatory activities were evaluated for triterpene and steroidal saponins using lipopolysaccharide-stimulated RAW264.7 macrophages and THP-1 monocyte leukemia cells. Results: Phytochemical investigation of P. ginseng seeds led to the isolation of a novel triterpene saponin, pseudoginsenoside RT8, along with 15 known compounds. Pseudoginsenoside RT8 exhibited more potent antiinflammatory activity than the other saponins, attenuating lipopolysaccharide-mediated induction of proinflammatory genes such as interleukin-1β, interleukin-6, inducible nitric oxide synthase, cyclooxygenase-2, and matrix metalloproteinase-9, and suppressed reactive oxygen species and nitric oxide generation in a dose-dependent manner. Conclusion: These findings indicate that pseudoginsenoside RT8 has a pharmaceutical potential as an antiinflammatory agent and that P. ginseng seeds are a good natural source for discovering novel bioactive molecules. Keywords: Antiinflammatory activity, Panax ginseng seeds, Pseudoginsenoside RT8 |
url |
http://www.sciencedirect.com/science/article/pii/S1226845318301003 |
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