Co-Occurrence of Tetrodotoxin and Saxitoxins and Their Intra-Body Distribution in the Pufferfish <i>Canthigaster valentini</i>
Pufferfish of the family Tetraodontidae possess tetrodotoxin (TTX) and/or saxitoxins (STXs), but the toxin ratio differs, depending on the genus or species. In the present study, to clarify the distribution profile of TTX and STXs in Tetraodontidae, we investigated the composition and intra-body dis...
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doaj-cc861eaf863243b6a919c215053b3f982020-11-25T02:59:48ZengMDPI AGToxins2072-66512020-07-011243643610.3390/toxins12070436Co-Occurrence of Tetrodotoxin and Saxitoxins and Their Intra-Body Distribution in the Pufferfish <i>Canthigaster valentini</i>Hongchen Zhu0Takayuki Sonoyama1Misako Yamada2Wei Gao3Ryohei Tatsuno4Tomohiro Takatani5Osamu Arakawa6Graduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14, Bunkyo-machi, Nagasaki, Nagasaki 852-8521, JapanShimonoseki Marine Science Museum, 6-1, Arcaport, Shimonoseki, Yamaguchi 750-0036, JapanGraduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14, Bunkyo-machi, Nagasaki, Nagasaki 852-8521, JapanGraduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14, Bunkyo-machi, Nagasaki, Nagasaki 852-8521, JapanDepartment of Food Science and Technology, National Fisheries University, Japan Fisheries Research and Education Agency, 2-7-1, Nagatahonmachi, Shimonoseki, Yamaguchi 759-6595, JapanGraduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14, Bunkyo-machi, Nagasaki, Nagasaki 852-8521, JapanGraduate School of Fisheries and Environmental Sciences, Nagasaki University, 1-14, Bunkyo-machi, Nagasaki, Nagasaki 852-8521, JapanPufferfish of the family Tetraodontidae possess tetrodotoxin (TTX) and/or saxitoxins (STXs), but the toxin ratio differs, depending on the genus or species. In the present study, to clarify the distribution profile of TTX and STXs in Tetraodontidae, we investigated the composition and intra-body distribution of the toxins in <i>Canthigaster valentini</i>. <i>C. valentini</i> specimens (four male and six female) were collected from Amami-Oshima Island, Kagoshima Prefecture, Japan, and the toxins were extracted from the muscle, liver, intestine, gallbladder, gonads, and skin. Analysis of the extracts for TTX by liquid chromatography tandem mass spectrometry and of STXs by high-performance liquid chromatography with post-column fluorescence derivatization revealed TTX, as well as a large amount of STXs, with neoSTX as the main component and dicarbamoylSTX and STX itself as minor components, in the skin and ovary. The toxins were also detected in the other tissues, but in much lower amounts than in the skin and ovary. The TTX/STX ratio varied greatly, depending on the tissue, but TTX was the major toxin component in the whole body, and STXs accounted for 25% and 13% of the total toxin amount in males and females, respectively. Like the marine pufferfish of the genus <i>Arothron</i>, <i>C. valentini</i> should be considered a pufferfish with considerable amounts of both TTX and STXs present simultaneously.https://www.mdpi.com/2072-6651/12/7/436tetrodotoxin (TTX)saxitoxin (STX)pufferfish<i>Canthigaster valentini</i> |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Hongchen Zhu Takayuki Sonoyama Misako Yamada Wei Gao Ryohei Tatsuno Tomohiro Takatani Osamu Arakawa |
spellingShingle |
Hongchen Zhu Takayuki Sonoyama Misako Yamada Wei Gao Ryohei Tatsuno Tomohiro Takatani Osamu Arakawa Co-Occurrence of Tetrodotoxin and Saxitoxins and Their Intra-Body Distribution in the Pufferfish <i>Canthigaster valentini</i> Toxins tetrodotoxin (TTX) saxitoxin (STX) pufferfish <i>Canthigaster valentini</i> |
author_facet |
Hongchen Zhu Takayuki Sonoyama Misako Yamada Wei Gao Ryohei Tatsuno Tomohiro Takatani Osamu Arakawa |
author_sort |
Hongchen Zhu |
title |
Co-Occurrence of Tetrodotoxin and Saxitoxins and Their Intra-Body Distribution in the Pufferfish <i>Canthigaster valentini</i> |
title_short |
Co-Occurrence of Tetrodotoxin and Saxitoxins and Their Intra-Body Distribution in the Pufferfish <i>Canthigaster valentini</i> |
title_full |
Co-Occurrence of Tetrodotoxin and Saxitoxins and Their Intra-Body Distribution in the Pufferfish <i>Canthigaster valentini</i> |
title_fullStr |
Co-Occurrence of Tetrodotoxin and Saxitoxins and Their Intra-Body Distribution in the Pufferfish <i>Canthigaster valentini</i> |
title_full_unstemmed |
Co-Occurrence of Tetrodotoxin and Saxitoxins and Their Intra-Body Distribution in the Pufferfish <i>Canthigaster valentini</i> |
title_sort |
co-occurrence of tetrodotoxin and saxitoxins and their intra-body distribution in the pufferfish <i>canthigaster valentini</i> |
publisher |
MDPI AG |
series |
Toxins |
issn |
2072-6651 |
publishDate |
2020-07-01 |
description |
Pufferfish of the family Tetraodontidae possess tetrodotoxin (TTX) and/or saxitoxins (STXs), but the toxin ratio differs, depending on the genus or species. In the present study, to clarify the distribution profile of TTX and STXs in Tetraodontidae, we investigated the composition and intra-body distribution of the toxins in <i>Canthigaster valentini</i>. <i>C. valentini</i> specimens (four male and six female) were collected from Amami-Oshima Island, Kagoshima Prefecture, Japan, and the toxins were extracted from the muscle, liver, intestine, gallbladder, gonads, and skin. Analysis of the extracts for TTX by liquid chromatography tandem mass spectrometry and of STXs by high-performance liquid chromatography with post-column fluorescence derivatization revealed TTX, as well as a large amount of STXs, with neoSTX as the main component and dicarbamoylSTX and STX itself as minor components, in the skin and ovary. The toxins were also detected in the other tissues, but in much lower amounts than in the skin and ovary. The TTX/STX ratio varied greatly, depending on the tissue, but TTX was the major toxin component in the whole body, and STXs accounted for 25% and 13% of the total toxin amount in males and females, respectively. Like the marine pufferfish of the genus <i>Arothron</i>, <i>C. valentini</i> should be considered a pufferfish with considerable amounts of both TTX and STXs present simultaneously. |
topic |
tetrodotoxin (TTX) saxitoxin (STX) pufferfish <i>Canthigaster valentini</i> |
url |
https://www.mdpi.com/2072-6651/12/7/436 |
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