Identification and Validation of Reference Genes in Clostridium beijerinckii NRRL B-598 for RT-qPCR Using RNA-Seq Data

Identification and Validation of Reference Genes in Clostridium beijerinckii NRRL B-598 for RT-qPCR Using RNA-Seq Data

Gene expression analysis through reverse transcription-quantitative real-time polymerase chain reaction (RT-qPCR) depends on correct data normalization by reference genes with stable expression. Although Clostridium beijerinckii NRRL B-598 is a promising Gram-positive bacterium for the industrial pr...

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Bibliographic Details
Main Authors: Katerina Jureckova, Hana Raschmanova, Jan Kolek, Maryna Vasylkivska, Barbora Branska, Petra Patakova, Ivo Provaznik, Karel Sedlar
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-03-01
Series:Frontiers in Microbiology
Subjects:
HKG
housekeeping genes
non-model organisms
biofuel
Clostridium
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.640054/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2021.640054/full

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