Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography
pKa values of five β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using the RP-HPLC method. Stationary phase was octadecyl modified (C-18) silica gel, and mobile phase was a mixture of methanol and one of nine different buffers (60:40, v/v). wspH values were measured after mixing metha...
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University of Kragujevac, Faculty of Science
2018-01-01
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doaj-cd6aa3cb5645465984d539d378b8ead62020-11-24T21:23:13ZengUniversity of Kragujevac, Faculty of ScienceKragujevac Journal of Science1450-96362466-55092018-01-012018401031111450-96361840103SDetermination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatographySavić Jelena S.0Marković Bojan1Vitnik Vesna2Dilber Sanda P.3University of Belgrade, Faculty of Pharmacy, BelgradeUniversity of Belgrade, Faculty of Pharmacy, BelgradeUniversity of Belgrade-ICTM, BelgradeUniversity of Belgrade, Faculty of Pharmacy, BelgradepKa values of five β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using the RP-HPLC method. Stationary phase was octadecyl modified (C-18) silica gel, and mobile phase was a mixture of methanol and one of nine different buffers (60:40, v/v). wspH values were measured after mixing methanol with an appropriate buffer. The mean retention time of each compound was plotted against wspH of each mobile phase. The inflection point of each sigmoidal curve represented wspK a of the compound. Using wspK a in already known equations for the specific methanol/buffer mixture, wwpK a values were calculated. Obtained pKa values for synthesized compounds were in a narrow range from 3.34-3.81 and pKa for ibuprofen was 4.45. Predicted pKa values for these compounds in SPARC software showed good correlation with experimental pKa values (R2=0.8048).https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2018/1450-96361840103S.pdfdissociation constantanti-inflammatory compoundsibuprofenliquid chromatography |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Savić Jelena S. Marković Bojan Vitnik Vesna Dilber Sanda P. |
spellingShingle |
Savić Jelena S. Marković Bojan Vitnik Vesna Dilber Sanda P. Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography Kragujevac Journal of Science dissociation constant anti-inflammatory compounds ibuprofen liquid chromatography |
author_facet |
Savić Jelena S. Marković Bojan Vitnik Vesna Dilber Sanda P. |
author_sort |
Savić Jelena S. |
title |
Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography |
title_short |
Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography |
title_full |
Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography |
title_fullStr |
Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography |
title_full_unstemmed |
Determination of ionization constants (PKA) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography |
title_sort |
determination of ionization constants (pka) of β-hydroxy-β-arylalkanoic acids using high-pressure liquid chromatography |
publisher |
University of Kragujevac, Faculty of Science |
series |
Kragujevac Journal of Science |
issn |
1450-9636 2466-5509 |
publishDate |
2018-01-01 |
description |
pKa values of five β-hydroxy-β-arylalkanoic acids and ibuprofen were determined using the RP-HPLC method. Stationary phase was octadecyl modified (C-18) silica gel, and mobile phase was a mixture of methanol and one of nine different buffers (60:40, v/v). wspH values were measured after mixing methanol with an appropriate buffer. The mean retention time of each compound was plotted against wspH of each mobile phase. The inflection point of each sigmoidal curve represented wspK a of the compound. Using wspK a in already known equations for the specific methanol/buffer mixture, wwpK a values were calculated. Obtained pKa values for synthesized compounds were in a narrow range from 3.34-3.81 and pKa for ibuprofen was 4.45. Predicted pKa values for these compounds in SPARC software showed good correlation with experimental pKa values (R2=0.8048). |
topic |
dissociation constant anti-inflammatory compounds ibuprofen liquid chromatography |
url |
https://scindeks-clanci.ceon.rs/data/pdf/1450-9636/2018/1450-96361840103S.pdf |
work_keys_str_mv |
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