Immobilization of pectinase by adsorption on an alginate-coated chitin support

Aspergillus niger pectinase was immobilized on an alginate-coated chitin support by adsorption. The optimal conditions for immobilization were: pH 4.5, time of incubation 2 h and 85 µg/mL protein concentration. The yield of immobilized protein was 70 % and the enzyme retained 60 % of the initial act...

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Bibliographic Details
Main Authors: Hector L Ramirez, Ana I Briones, Juan Úbeda, María Arevalo
Format: Article
Language:English
Published: Elfos Scientiae
Series:Biotecnología Aplicada
Subjects:
Online Access:http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1027-28522013000200003&lng=en&tlng=en