Droplet Digital PCR for Estimating Absolute Abundances of Widespread Pelagibacter Viruses

Droplet Digital PCR for Estimating Absolute Abundances of Widespread Pelagibacter Viruses

Absolute abundances of prokaryotes are typically determined by FISH. Due to the lack of a universal conserved gene among all viruses, metagenomic fragment recruitment is commonly used to estimate the relative viral abundance. However, the paucity of absolute virus abundance data hinders our ability...

Full description

Bibliographic Details
Main Authors: Francisco Martinez-Hernandez, Inmaculada Garcia-Heredia, Monica Lluesma Gomez, Lucia Maestre-Carballa, Joaquín Martínez Martínez, Manuel Martinez-Garcia
Format: Article
Language:English
Published: Frontiers Media S.A. 2019-06-01
Series:Frontiers in Microbiology
Subjects:
marine
ddPCR
Pelagibacter ubique
abundance
virus
single-virus genomics
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2019.01226/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2019.01226/full

Similar Items