Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing

Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing

Summary: Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer meta...

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Main Authors: Kei Takahashi, Shimpei I. Kubota, Shogo Ehata, Hiroki R. Ueda, Kohei Miyazono
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Cancer
Microscopy
Model Organisms
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301787
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spelling doaj-d1252c164822453196fcd6ed38c58f8a2020-12-21T04:49:06ZengElsevierSTAR Protocols2666-16672020-12-0113100191Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue ClearingKei Takahashi0Shimpei I. Kubota1Shogo Ehata2Hiroki R. Ueda3Kohei Miyazono4Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, JapanDepartment of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, JapanDepartment of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, JapanDepartment of Systems Pharmacology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Laboratory for Synthetic Biology, RIKEN Center for Biosystems Dynamics Research, 1-3, Yamadaoka, Suita, Osaka 565-0871, Japan; Corresponding authorDepartment of Molecular Pathology, Graduate School of Medicine, The University of Tokyo, 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-0033, Japan; Corresponding authorSummary: Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry.For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017).http://www.sciencedirect.com/science/article/pii/S2666166720301787CancerMicroscopyModel Organisms
collection DOAJ
language English
format Article
sources DOAJ
author Kei Takahashi
Shimpei I. Kubota
Shogo Ehata
Hiroki R. Ueda
Kohei Miyazono
spellingShingle Kei Takahashi
Shimpei I. Kubota
Shogo Ehata
Hiroki R. Ueda
Kohei Miyazono
Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
STAR Protocols
Cancer
Microscopy
Model Organisms
author_facet Kei Takahashi
Shimpei I. Kubota
Shogo Ehata
Hiroki R. Ueda
Kohei Miyazono
author_sort Kei Takahashi
title Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title_short Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title_full Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title_fullStr Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title_full_unstemmed Protocol for Imaging and Analysis of Mouse Tumor Models with CUBIC Tissue Clearing
title_sort protocol for imaging and analysis of mouse tumor models with cubic tissue clearing
publisher Elsevier
series STAR Protocols
issn 2666-1667
publishDate 2020-12-01
description Summary: Tissue-clearing technologies have developed rapidly in the past decade, especially for use in neuroscience research. We previously reported that CUBIC, which is one tissue-clearing method, is useful for applications in cancer research. CUBIC cancer analysis can be used to detect cancer metastasis with single-cell resolution at whole mouse body/organ level. This approach can also analyze the tumor characteristics with high-quality 3D images. Here, we describe a detailed CUBIC cancer protocol from tissue clearing, capturing 3D images and post-immunohistochemistry.For complete details on the use and execution of this protocol, please refer to Kubota et al. (2017).
topic Cancer
Microscopy
Model Organisms
url http://www.sciencedirect.com/science/article/pii/S2666166720301787
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