Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine
Apicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predic...
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Series: | Journal of Parasitology Research |
Online Access: | http://dx.doi.org/10.1155/2021/9974509 |
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doaj-d33813b0e0bc403394633621d3aab1fd2021-07-26T00:34:16ZengHindawi LimitedJournal of Parasitology Research2090-00312021-01-01202110.1155/2021/9974509Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii VaccineAli Taghipour0Sanaz Tavakoli1Mohamad Sabaghan2Masoud Foroutan3Hamidreza Majidiani4Shahrzad Soltani5Milad Badri6Ali Dalir Ghaffari7Sheyda Soltani8Department of ParasitologyDepartment of Parasitology and MycologyBehbahan Faculty of Medical SciencesUSERN OfficeZoonotic Diseases Research CenterUSERN OfficeMedical Microbiology Research CenterDepartment of ParasitologyUSERN OfficeApicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predict the basic features and specifications of the CDPK7 protein. The findings showed that CDPK7 protein has 2133 amino acid residues with an average molecular weight (MW) of 219085.79 D. The aliphatic index with 68.78 and grand average of hydropathicity (GRAVY) with -0.331 score were estimated. The outcomes of current research showed that the CDPK7 protein included 502 alpha-helix, 1311 random coils, and 320 extended strands with GOR4 method. Considering the Ramachandran plot, the favored region contains more than 92% of the amino acid residues. In addition, evaluation of antigenicity and allergenicity showed that CDPK7 protein has immunogenic and nonallergenic nature. The present research provides key data for more animal-model study on the CDPK7 protein to design an efficient vaccine against toxoplasmosis in the future.http://dx.doi.org/10.1155/2021/9974509 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ali Taghipour Sanaz Tavakoli Mohamad Sabaghan Masoud Foroutan Hamidreza Majidiani Shahrzad Soltani Milad Badri Ali Dalir Ghaffari Sheyda Soltani |
spellingShingle |
Ali Taghipour Sanaz Tavakoli Mohamad Sabaghan Masoud Foroutan Hamidreza Majidiani Shahrzad Soltani Milad Badri Ali Dalir Ghaffari Sheyda Soltani Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine Journal of Parasitology Research |
author_facet |
Ali Taghipour Sanaz Tavakoli Mohamad Sabaghan Masoud Foroutan Hamidreza Majidiani Shahrzad Soltani Milad Badri Ali Dalir Ghaffari Sheyda Soltani |
author_sort |
Ali Taghipour |
title |
Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine |
title_short |
Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine |
title_full |
Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine |
title_fullStr |
Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine |
title_full_unstemmed |
Immunoinformatic Analysis of Calcium-Dependent Protein Kinase 7 (CDPK7) Showed Potential Targets for Toxoplasma gondii Vaccine |
title_sort |
immunoinformatic analysis of calcium-dependent protein kinase 7 (cdpk7) showed potential targets for toxoplasma gondii vaccine |
publisher |
Hindawi Limited |
series |
Journal of Parasitology Research |
issn |
2090-0031 |
publishDate |
2021-01-01 |
description |
Apicomplexan parasites, including Toxoplasma gondii (T. gondii), express different types of calcium-dependent protein kinases (CDPKs), which perform a variety of functions, including attacking and exiting the host cells. In the current bioinformatics study, we have used several web servers to predict the basic features and specifications of the CDPK7 protein. The findings showed that CDPK7 protein has 2133 amino acid residues with an average molecular weight (MW) of 219085.79 D. The aliphatic index with 68.78 and grand average of hydropathicity (GRAVY) with -0.331 score were estimated. The outcomes of current research showed that the CDPK7 protein included 502 alpha-helix, 1311 random coils, and 320 extended strands with GOR4 method. Considering the Ramachandran plot, the favored region contains more than 92% of the amino acid residues. In addition, evaluation of antigenicity and allergenicity showed that CDPK7 protein has immunogenic and nonallergenic nature. The present research provides key data for more animal-model study on the CDPK7 protein to design an efficient vaccine against toxoplasmosis in the future. |
url |
http://dx.doi.org/10.1155/2021/9974509 |
work_keys_str_mv |
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