MALS: an efficient strategy for multiple site-directed mutagenesis employing a combination of DNA amplification, ligation and suppression PCR

<p>Abstract</p> <p>Background</p> <p>Multiple approaches for the site-directed mutagenesis (SDM) have been developed. However, only several of them are designed for simultaneous introduction of multiple nucleotide alterations, and these are time consuming. In addition,...

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Bibliographic Details
Main Authors: Drayna Dennis T, Fushan Alexey A
Format: Article
Language:English
Published: BMC 2009-09-01
Series:BMC Biotechnology
Online Access:http://www.biomedcentral.com/1472-6750/9/83