CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation

CRISPR/Cas9 mediated CXCL4 knockout in human iPS cells of polycythemia vera patient with JAK2 V617F mutation

The chemokine CXCL4/platelet factor 4 (PF4) gene, a key player in myelofibrosis, was knocked out by CRISPR/Cas9 in induced pluripotent stem cells (iPS cells) of a polycythemia vera (PV) patient with JAK2 V617F mutation. Two CXCL4KO iPS cell lines with and without JAK2 V617F mutation (UKAi002-B-1 and...

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Bibliographic Details
Main Authors: Janik Boehnke, Salim Atakhanov, Marcelo A.S. Toledo, Herdit M. Schüler, Stephanie Sontag, Nicolas Chatain, Steffen Koschmieder, Tim H. Brümmendorf, Rafael Kramann, Martin Zenke
Format: Article
Language:English
Published: Elsevier 2021-08-01
Series:Stem Cell Research
Subjects:
Induced pluripotent stem cell
iPS cells
Hematopoiesis
CXCL4
PF4
JAK2 V617F
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506121003378

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http://www.sciencedirect.com/science/article/pii/S1873506121003378

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