Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysis
Abstract Tuberculosis (TB) is the leading cause of death in humans by a single infectious agent worldwide with approximately two billion humans latently infected with the bacterium Mycobacterium tuberculosis. Currently, the accepted method for controlling the disease is Tuberculosis Directly Observe...
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doaj-d5ce7e21702d40abb30a9fa3e53c6c622021-06-28T07:05:59ZengWileyMicrobiologyOpen2045-88272021-06-01103n/an/a10.1002/mbo3.1211Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysisEdriss Yassine0Ronald Galiwango1Willy Ssengooba2Fred Ashaba3Moses L. Joloba4Sarah Zalwango5Christopher C. Whalen6Frederick Quinn7Department of Infectious Diseases College of Veterinary Medicine University of Georgia Athens GA USADepartment of Epidemiology and Biostatistics College of Public Health University of Georgia Athens GA USAMakerere University Lung Institute College of Health Sciences Makerere University Kampala UgandaUganda‐CWRU Research Collaboration Makerere University and Mulago Hospital Kampala UgandaUganda‐CWRU Research Collaboration Makerere University and Mulago Hospital Kampala UgandaUganda‐CWRU Research Collaboration Makerere University and Mulago Hospital Kampala UgandaDepartment of Epidemiology and Biostatistics College of Public Health University of Georgia Athens GA USADepartment of Infectious Diseases College of Veterinary Medicine University of Georgia Athens GA USAAbstract Tuberculosis (TB) is the leading cause of death in humans by a single infectious agent worldwide with approximately two billion humans latently infected with the bacterium Mycobacterium tuberculosis. Currently, the accepted method for controlling the disease is Tuberculosis Directly Observed Treatment Shortcourse (TB‐DOTS). This program is not preventative and individuals may transmit disease before diagnosis, thus better understanding of disease transmission is essential. Using whole‐genome sequencing and single nucleotide polymorphism analysis, we analyzed genomes of 145 M. tuberculosis clinical isolates from active TB cases from the Rubaga Division of Kampala, Uganda. We established that these isolates grouped into M. tuberculosis complex (MTBC) lineages 1, 2, 3, and 4, with the most isolates grouping into lineage 4. Possible transmission pairs containing ≤12 SNPs were identified in lineages 1, 3, and 4 with the prevailing transmission in lineages 3 and 4. Furthermore, investigating DNA codon changes as a result of specific SNPs in prominent virulence genes including plcA and plcB could indicate potentially important modifications in protein function. Incorporating this analysis with corresponding epidemiological data may provide a blueprint for the integration of public health interventions to decrease TB transmission in a region.https://doi.org/10.1002/mbo3.1211Mycobacterium tuberculosissingle nucleotide polymorphismsocial networktransmissiontuberculosis |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Edriss Yassine Ronald Galiwango Willy Ssengooba Fred Ashaba Moses L. Joloba Sarah Zalwango Christopher C. Whalen Frederick Quinn |
spellingShingle |
Edriss Yassine Ronald Galiwango Willy Ssengooba Fred Ashaba Moses L. Joloba Sarah Zalwango Christopher C. Whalen Frederick Quinn Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysis MicrobiologyOpen Mycobacterium tuberculosis single nucleotide polymorphism social network transmission tuberculosis |
author_facet |
Edriss Yassine Ronald Galiwango Willy Ssengooba Fred Ashaba Moses L. Joloba Sarah Zalwango Christopher C. Whalen Frederick Quinn |
author_sort |
Edriss Yassine |
title |
Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysis |
title_short |
Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysis |
title_full |
Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysis |
title_fullStr |
Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysis |
title_full_unstemmed |
Assessing a transmission network of Mycobacterium tuberculosis in an African city using single nucleotide polymorphism threshold analysis |
title_sort |
assessing a transmission network of mycobacterium tuberculosis in an african city using single nucleotide polymorphism threshold analysis |
publisher |
Wiley |
series |
MicrobiologyOpen |
issn |
2045-8827 |
publishDate |
2021-06-01 |
description |
Abstract Tuberculosis (TB) is the leading cause of death in humans by a single infectious agent worldwide with approximately two billion humans latently infected with the bacterium Mycobacterium tuberculosis. Currently, the accepted method for controlling the disease is Tuberculosis Directly Observed Treatment Shortcourse (TB‐DOTS). This program is not preventative and individuals may transmit disease before diagnosis, thus better understanding of disease transmission is essential. Using whole‐genome sequencing and single nucleotide polymorphism analysis, we analyzed genomes of 145 M. tuberculosis clinical isolates from active TB cases from the Rubaga Division of Kampala, Uganda. We established that these isolates grouped into M. tuberculosis complex (MTBC) lineages 1, 2, 3, and 4, with the most isolates grouping into lineage 4. Possible transmission pairs containing ≤12 SNPs were identified in lineages 1, 3, and 4 with the prevailing transmission in lineages 3 and 4. Furthermore, investigating DNA codon changes as a result of specific SNPs in prominent virulence genes including plcA and plcB could indicate potentially important modifications in protein function. Incorporating this analysis with corresponding epidemiological data may provide a blueprint for the integration of public health interventions to decrease TB transmission in a region. |
topic |
Mycobacterium tuberculosis single nucleotide polymorphism social network transmission tuberculosis |
url |
https://doi.org/10.1002/mbo3.1211 |
work_keys_str_mv |
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