Cellular Response to Individual Components of the Platelet Concentrate
Platelet concentrates and especially their further product platelet lysate, are widely used as a replacement for cell culturing. Platelets contain a broad spectrum of growth factors and bioactive molecules that affect cellular fate. However, the cellular response to individual components of the huma...
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doaj-d6067f6d94f3467cbee8fc8d2b2011a02021-04-26T23:05:12ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-04-01224539453910.3390/ijms22094539Cellular Response to Individual Components of the Platelet ConcentrateVera Sovkova0Karolina Vocetkova1Věra Hedvičáková2Veronika Hefka Blahnová3Matěj Buzgo4Evzen Amler5Eva Filová6Department of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech RepublicDepartment of Tissue Engineering, Institute of Experimental Medicine, The Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicDepartment of Tissue Engineering, Institute of Experimental Medicine, The Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicDepartment of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech RepublicUniversity Centre for Energy Efficient Buildings, Czech Technical University in Prague, Trinecka 1024, 273 43 Bustehrad, Czech RepublicDepartment of Biophysics, 2nd Faculty of Medicine, Charles University, V Uvalu 84, 150 06 Prague, Czech RepublicDepartment of Tissue Engineering, Institute of Experimental Medicine, The Czech Academy of Sciences, Videnska 1083, 142 20 Prague, Czech RepublicPlatelet concentrates and especially their further product platelet lysate, are widely used as a replacement for cell culturing. Platelets contain a broad spectrum of growth factors and bioactive molecules that affect cellular fate. However, the cellular response to individual components of the human platelet concentrate is still unclear. The aim of this study was to observe cellular behavior according to the individual components of platelet concentrates. The bioactive molecule content was determined. The cells were supplemented with a medium containing 8% (<i>v</i>/<i>v</i>) of platelet proteins in plasma, pure platelet proteins in deionized water, and pure plasma. The results showed a higher concentration of fibrinogen, albumin, insulin growth factor I (IGF-1), keratinocyte growth factor (KGF), and hepatocyte growth factor (HGF), in the groups containing plasma. On the other hand, chemokine RANTES and platelet-derived growth factor bb (PDGF-bb), were higher in the groups containing platelet proteins. The groups containing both plasma and plasma proteins showed the most pronounced proliferation and viability of mesenchymal stem cells and fibroblasts. The platelet proteins alone were not sufficient to provide optimal cell growth and viability. A synergic effect of platelet proteins and plasma was observed. The data indicated the importance of plasma in platelet lysate for cell growth.https://www.mdpi.com/1422-0067/22/9/4539plateletsplasmamesenchymal stem cellsfibroblasts |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vera Sovkova Karolina Vocetkova Věra Hedvičáková Veronika Hefka Blahnová Matěj Buzgo Evzen Amler Eva Filová |
spellingShingle |
Vera Sovkova Karolina Vocetkova Věra Hedvičáková Veronika Hefka Blahnová Matěj Buzgo Evzen Amler Eva Filová Cellular Response to Individual Components of the Platelet Concentrate International Journal of Molecular Sciences platelets plasma mesenchymal stem cells fibroblasts |
author_facet |
Vera Sovkova Karolina Vocetkova Věra Hedvičáková Veronika Hefka Blahnová Matěj Buzgo Evzen Amler Eva Filová |
author_sort |
Vera Sovkova |
title |
Cellular Response to Individual Components of the Platelet Concentrate |
title_short |
Cellular Response to Individual Components of the Platelet Concentrate |
title_full |
Cellular Response to Individual Components of the Platelet Concentrate |
title_fullStr |
Cellular Response to Individual Components of the Platelet Concentrate |
title_full_unstemmed |
Cellular Response to Individual Components of the Platelet Concentrate |
title_sort |
cellular response to individual components of the platelet concentrate |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2021-04-01 |
description |
Platelet concentrates and especially their further product platelet lysate, are widely used as a replacement for cell culturing. Platelets contain a broad spectrum of growth factors and bioactive molecules that affect cellular fate. However, the cellular response to individual components of the human platelet concentrate is still unclear. The aim of this study was to observe cellular behavior according to the individual components of platelet concentrates. The bioactive molecule content was determined. The cells were supplemented with a medium containing 8% (<i>v</i>/<i>v</i>) of platelet proteins in plasma, pure platelet proteins in deionized water, and pure plasma. The results showed a higher concentration of fibrinogen, albumin, insulin growth factor I (IGF-1), keratinocyte growth factor (KGF), and hepatocyte growth factor (HGF), in the groups containing plasma. On the other hand, chemokine RANTES and platelet-derived growth factor bb (PDGF-bb), were higher in the groups containing platelet proteins. The groups containing both plasma and plasma proteins showed the most pronounced proliferation and viability of mesenchymal stem cells and fibroblasts. The platelet proteins alone were not sufficient to provide optimal cell growth and viability. A synergic effect of platelet proteins and plasma was observed. The data indicated the importance of plasma in platelet lysate for cell growth. |
topic |
platelets plasma mesenchymal stem cells fibroblasts |
url |
https://www.mdpi.com/1422-0067/22/9/4539 |
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