Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo
The modern stopped-flow reaction analyzer has shown high efficiency and flexibility, which provides outstanding sample economy with a dead-time of less than 1 ms. However the cost of the equipment imposes a serious restriction to many Brazilian scientists and teachers. In this work we describe the c...
Main Authors: | , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
Sociedade Brasileira de Química
2006-10-01
|
Series: | Química Nova |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500034&lng=en&tlng=en |
id |
doaj-d764ede5bc384d7496d8b2820d7f0fd0 |
---|---|
record_format |
Article |
spelling |
doaj-d764ede5bc384d7496d8b2820d7f0fd02020-11-24T21:02:26ZengSociedade Brasileira de QuímicaQuímica Nova1678-70642006-10-012951101110510.1590/S0100-40422006000500034S0100-40422006000500034Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custoVagner Roberto Batistela0André Luiz Tessaro1Rafael R. da S. Soares2Vagner Roberto de Souza3Noboru Hioka4Universidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáUniversidade Estadual de MaringáThe modern stopped-flow reaction analyzer has shown high efficiency and flexibility, which provides outstanding sample economy with a dead-time of less than 1 ms. However the cost of the equipment imposes a serious restriction to many Brazilian scientists and teachers. In this work we describe the construction of a low-cost stopped-flow system coupled to a UV-Vis spectrophotometer. The performance of the system was checked by monitoring the kinetics of two reactions: the fading of phenolphthalein in aqueous alkaline solution and the chlorophyll a demetallation in acid medium. The apparatus showed reasonable efficiency with a dead-time of 0.3 to 0.5 s. The very good results obtained in these two illustrative processes show that the system is satisfactory for determining rate constants with mean reaction times ranging from seconds to minutes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500034&lng=en&tlng=enstopped-flowkineticsfast reactions |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Vagner Roberto Batistela André Luiz Tessaro Rafael R. da S. Soares Vagner Roberto de Souza Noboru Hioka |
spellingShingle |
Vagner Roberto Batistela André Luiz Tessaro Rafael R. da S. Soares Vagner Roberto de Souza Noboru Hioka Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo Química Nova stopped-flow kinetics fast reactions |
author_facet |
Vagner Roberto Batistela André Luiz Tessaro Rafael R. da S. Soares Vagner Roberto de Souza Noboru Hioka |
author_sort |
Vagner Roberto Batistela |
title |
Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo |
title_short |
Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo |
title_full |
Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo |
title_fullStr |
Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo |
title_full_unstemmed |
Um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo |
title_sort |
um aparato para monitorar reações rápidas: um "stopped-flow" artesanal de baixo custo |
publisher |
Sociedade Brasileira de Química |
series |
Química Nova |
issn |
1678-7064 |
publishDate |
2006-10-01 |
description |
The modern stopped-flow reaction analyzer has shown high efficiency and flexibility, which provides outstanding sample economy with a dead-time of less than 1 ms. However the cost of the equipment imposes a serious restriction to many Brazilian scientists and teachers. In this work we describe the construction of a low-cost stopped-flow system coupled to a UV-Vis spectrophotometer. The performance of the system was checked by monitoring the kinetics of two reactions: the fading of phenolphthalein in aqueous alkaline solution and the chlorophyll a demetallation in acid medium. The apparatus showed reasonable efficiency with a dead-time of 0.3 to 0.5 s. The very good results obtained in these two illustrative processes show that the system is satisfactory for determining rate constants with mean reaction times ranging from seconds to minutes. |
topic |
stopped-flow kinetics fast reactions |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-40422006000500034&lng=en&tlng=en |
work_keys_str_mv |
AT vagnerrobertobatistela umaparatoparamonitorarreacoesrapidasumstoppedflowartesanaldebaixocusto AT andreluiztessaro umaparatoparamonitorarreacoesrapidasumstoppedflowartesanaldebaixocusto AT rafaelrdassoares umaparatoparamonitorarreacoesrapidasumstoppedflowartesanaldebaixocusto AT vagnerrobertodesouza umaparatoparamonitorarreacoesrapidasumstoppedflowartesanaldebaixocusto AT noboruhioka umaparatoparamonitorarreacoesrapidasumstoppedflowartesanaldebaixocusto |
_version_ |
1716775407258173440 |