Control for stochastic sampling variation and qualitative sequencing error in next generation sequencing

Control for stochastic sampling variation and qualitative sequencing error in next generation sequencing

Background: Clinical implementation of Next-Generation Sequencing (NGS) is challenged by poor control for stochastic sampling, library preparation biases and qualitative sequencing error. To address these challenges we developed and tested two hypotheses. Methods: Hypothesis 1: Analytical variation...

Full description

Bibliographic Details
Main Authors: Thomas Blomquist, Erin L. Crawford, Jiyoun Yeo, Xiaolu Zhang, James C. Willey
Format: Article
Language:English
Published: Elsevier 2015-09-01
Series:Biomolecular Detection and Quantification
Subjects:
NGS
Targeted
Sequencing
Diagnostics
Internal standards
Online Access:http://www.sciencedirect.com/science/article/pii/S221475351530005X

Internet

http://www.sciencedirect.com/science/article/pii/S221475351530005X

Similar Items