Callus induction in Hydnocarpus pentandra (Buch. Ham.) Oken using shoot tips

• Main Author: Vighnesha Bhat, P. E. Rajasekharan, G. Raviraja Shetty, Sadashiv Nadukeri and M. Ganapati
• Format: Article
• Language: English
• Published: Indian Society of Plant Breeders 2020-03-01
• Series: Electronic Journal of Plant Breeding
• Subjects: vulnerable medicinal tree; growth hormones; explant; callus induction

Hydnocarpus pentandra (Buch. Ham.) Oken is a Vulnerable medicinal tree found in Western Ghats of India. It is highly exploited due to its medicinal properties and it is mainly used in leprosy treatment. An experiment was undertaken at Division of Plant Genetic Resources, ICAR-Indian Institute of Horticultural Sciences, Bengaluru during 2018-19. The experiment was laid down in Completely Randomized Design (CRD) with 16 treatments and three replications. Shoot tips from young seedlings were used as explant and treated in MS media with a different hormonal combination like Benzyl Amino Purine (BAP), Indole Acetic Acid (IAA), Kinetin and Thiaduzerol (TDZ). Among the treatments, highest shoot length (2.06 cm) and maximum number of shoots produced per explant (3.86) were found in the treatment combination of BAP (1.0 mg/l) and TDZ (0.5 mg/l) followed by the treatment combination of BAP (2.4 mg/l) and IAA (0.05 mg/l). Few treatments showed callus formation but no shoots were developed in these treatments.