A method for characterizing the thermal stability and antimicrobial binding to Lipopolysaccharides of Gram-negative isogenic mutant strains

A method for characterizing the thermal stability and antimicrobial binding to Lipopolysaccharides of Gram-negative isogenic mutant strains

Isothermal Titration Calorimetry (ITC) is widely employed to assess antimicrobial affinity for lipopolysaccharide (LPS); nevertheless, experiments are usually limited to commercially available-LPS chemotypes. Herein we show a method that uses Differential Scanning Calorimetry (DSC) to characterize h...

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Bibliographic Details
Main Authors: Belén Navarro, Mackarenna Alarcón, Maricarmen Osees, Felipe Gómez-Alvear, Romina V. Sepúlveda, Jaime Huerta, María Cecilia Opazo, Daniel Aguayo
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:MethodsX
Subjects:
Differential Scanning Calorimetry (ITC)
Isothermal Titration Calorimetry (ITC)
Antimicrobial binding
Lipopolysaccharides
Online Access:http://www.sciencedirect.com/science/article/pii/S2215016121002673

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