Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis

Acetylcholine is the neurotransmitter responsible for the transmission of impulses from cholinergic neurons to cells of innervated tissues. Its biosynthesis is catalyzed by the enzyme Choline acetyltransferase that is considered to be a phenotypically specific marker for cholinergic system. It is we...

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Main Authors: NILSON NUNES-TAVARES, NARCISA LEAL CUNHA-E-SILVA, AÍDA HASSÓN-VOLOCH
Format: Article
Language:English
Published: Academia Brasileira de Ciências 2000-09-01
Series:Anais da Academia Brasileira de Ciências
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300007
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spelling doaj-d967f25e76234678876c5ce4a937c4f62020-11-24T23:21:40ZengAcademia Brasileira de CiênciasAnais da Academia Brasileira de Ciências0001-37651678-26902000-09-0172333134010.1590/S0001-37652000000300007Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy AnalysisNILSON NUNES-TAVARESNARCISA LEAL CUNHA-E-SILVAAÍDA HASSÓN-VOLOCHAcetylcholine is the neurotransmitter responsible for the transmission of impulses from cholinergic neurons to cells of innervated tissues. Its biosynthesis is catalyzed by the enzyme Choline acetyltransferase that is considered to be a phenotypically specific marker for cholinergic system. It is well known that the regulation of Choline acetyltransferase activity under physiological and pathological conditions is important for development and neuronal activities of cholinergic functions. We observed the distribution of Choline acetyltransferase in sections from the normal and denervated main electric organ sections of Electrophorus electricus (L.) by immunofluorescence using a anti-Choline acetyltransferase antibody. The animals were submitted to a surgical procedure to remove about 20 nerves and after 30 and 60 days, they were sacrificed. After 30 days, the results from immunohistochemistry demonstrated an increase on the Choline acetyltransferase distribution at denervated tissue sections when compared with the sections from the normal contralateral organ. A very similar labeling was observed between normal and denervated tissue sections of the animals after 60 days. However, Choline acetyltransferase activity (nmolesACh/ min/ mg of protein) in extracts obtained from electrocyte microsomal preparation, estimated by Fonnun's method (Fonnun 1975), was 70% lower in the denervated extracts.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300007choline acetyltransferaseelectrocytedenervationimmunohistochemistry
collection DOAJ
language English
format Article
sources DOAJ
author NILSON NUNES-TAVARES
NARCISA LEAL CUNHA-E-SILVA
AÍDA HASSÓN-VOLOCH
spellingShingle NILSON NUNES-TAVARES
NARCISA LEAL CUNHA-E-SILVA
AÍDA HASSÓN-VOLOCH
Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis
Anais da Academia Brasileira de Ciências
choline acetyltransferase
electrocyte
denervation
immunohistochemistry
author_facet NILSON NUNES-TAVARES
NARCISA LEAL CUNHA-E-SILVA
AÍDA HASSÓN-VOLOCH
author_sort NILSON NUNES-TAVARES
title Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis
title_short Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis
title_full Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis
title_fullStr Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis
title_full_unstemmed Choline acetyltransferase detection in normal and denervated electrocyte from Electrophorus electricus (L.) using a Confocal Scanning Optical Microscopy Analysis
title_sort choline acetyltransferase detection in normal and denervated electrocyte from electrophorus electricus (l.) using a confocal scanning optical microscopy analysis
publisher Academia Brasileira de Ciências
series Anais da Academia Brasileira de Ciências
issn 0001-3765
1678-2690
publishDate 2000-09-01
description Acetylcholine is the neurotransmitter responsible for the transmission of impulses from cholinergic neurons to cells of innervated tissues. Its biosynthesis is catalyzed by the enzyme Choline acetyltransferase that is considered to be a phenotypically specific marker for cholinergic system. It is well known that the regulation of Choline acetyltransferase activity under physiological and pathological conditions is important for development and neuronal activities of cholinergic functions. We observed the distribution of Choline acetyltransferase in sections from the normal and denervated main electric organ sections of Electrophorus electricus (L.) by immunofluorescence using a anti-Choline acetyltransferase antibody. The animals were submitted to a surgical procedure to remove about 20 nerves and after 30 and 60 days, they were sacrificed. After 30 days, the results from immunohistochemistry demonstrated an increase on the Choline acetyltransferase distribution at denervated tissue sections when compared with the sections from the normal contralateral organ. A very similar labeling was observed between normal and denervated tissue sections of the animals after 60 days. However, Choline acetyltransferase activity (nmolesACh/ min/ mg of protein) in extracts obtained from electrocyte microsomal preparation, estimated by Fonnun's method (Fonnun 1975), was 70% lower in the denervated extracts.
topic choline acetyltransferase
electrocyte
denervation
immunohistochemistry
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652000000300007
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AT narcisalealcunhaesilva cholineacetyltransferasedetectioninnormalanddenervatedelectrocytefromelectrophoruselectricuslusingaconfocalscanningopticalmicroscopyanalysis
AT aidahassonvoloch cholineacetyltransferasedetectioninnormalanddenervatedelectrocytefromelectrophoruselectricuslusingaconfocalscanningopticalmicroscopyanalysis
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