Study of in vitro transcriptional binding effects and noise using constitutive promoters combined with UP element sequences in Escherichia coli

Study of in vitro transcriptional binding effects and noise using constitutive promoters combined with UP element sequences in Escherichia coli

Abstract Background UP elements (upstream element) are DNA sequences upstream of a promoter that interact with the α-subunit of RNA polymerase (RNAP) and can affect transcription by altering the binding RNAP to DNA. However, details of UP element and binding affinity effects on transcriptional stren...

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Bibliographic Details
Main Authors: Qiang Yan, Stephen S. Fong
Format: Article
Language:English
Published: BMC 2017-11-01
Series:Journal of Biological Engineering
Subjects:
Upstream element sequence
RNAP α subunits
Gene expression noise
Promoter strength
RNA polymerase
Binding association constant
Online Access:http://link.springer.com/article/10.1186/s13036-017-0075-2

Internet

http://link.springer.com/article/10.1186/s13036-017-0075-2

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