Regulatory Diversity and Functional Analysis of Two-Component Systems in Cyanobacterium Synechocystis sp. PCC 6803 by GC-MS Based Metabolomics
Two-component signal transduction systems are still poorly functionally characterized in the model cyanobacterium Synechocystis sp. PCC 6803. To address the issue, a GC-MS based comparative metabolomic analysis was conducted on a library of 44 knockout mutants for the response regulators (RRs) in Sy...
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doaj-dc225705a1f545e58fea920a0ebefbe02020-11-25T03:03:00ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-03-011110.3389/fmicb.2020.00403495627Regulatory Diversity and Functional Analysis of Two-Component Systems in Cyanobacterium Synechocystis sp. PCC 6803 by GC-MS Based MetabolomicsMengliang Shi0Mengliang Shi1Mengliang Shi2Lei Chen3Lei Chen4Lei Chen5Weiwen Zhang6Weiwen Zhang7Weiwen Zhang8Weiwen Zhang9Laboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, ChinaFrontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, ChinaFrontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering & Technology, Tianjin University, Tianjin, ChinaFrontier Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaCenter for Biosafety Research and Strategy, Tianjin University, Tianjin, ChinaTwo-component signal transduction systems are still poorly functionally characterized in the model cyanobacterium Synechocystis sp. PCC 6803. To address the issue, a GC-MS based comparative metabolomic analysis was conducted on a library of 44 knockout mutants for the response regulators (RRs) in Synechocystis. The metabolomic profiling analysis showed that 7 RRs mutants, namely Δslr1909, Δsll1291, Δslr6040, Δsll1330, Δslr2024, Δslr1584, and Δslr1693, were significantly different at metabolomic level, although their growth patterns are similar to the wild type under the normal autotrophic growth condition, suggesting regulatory diversity of RRs at metabolite level in Synechocystis. Additionally, a detailed metabolomic analysis coupled with RT-PCR verification led to useful clues for possible function of these 7 RRs, which were found involved in regulation of multiple aspects of cellular metabolisms in Synechocystis. Moreover, an integrative metabolomic and evolutionary analysis of all RR showed that four groups of RR genes clustered together in both metabolomic and evolutionary trees, suggesting of possible functional conservation of these RRs during the evolutionary process. Meanwhile, six groups of RRs with close evolutionary origin were found with different metabolomic profiles, suggesting possible functional changes during evolution. In contrast, more than 10 groups of RR genes with different clustering patterns in the evolutionary tree were found clustered together in metabolomics-based tree, suggesting possible functional convergences during the evolution. This study provided a metabolomic view of RR function, and the most needed functional clues for further characterization of these regulatory proteins in Synechocystis.https://www.frontiersin.org/article/10.3389/fmicb.2020.00403/fullSynechocystisGC-MSmetabolomicstwo-component signal transduction systemsresponse regulators |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Mengliang Shi Mengliang Shi Mengliang Shi Lei Chen Lei Chen Lei Chen Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang |
spellingShingle |
Mengliang Shi Mengliang Shi Mengliang Shi Lei Chen Lei Chen Lei Chen Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang Regulatory Diversity and Functional Analysis of Two-Component Systems in Cyanobacterium Synechocystis sp. PCC 6803 by GC-MS Based Metabolomics Frontiers in Microbiology Synechocystis GC-MS metabolomics two-component signal transduction systems response regulators |
author_facet |
Mengliang Shi Mengliang Shi Mengliang Shi Lei Chen Lei Chen Lei Chen Weiwen Zhang Weiwen Zhang Weiwen Zhang Weiwen Zhang |
author_sort |
Mengliang Shi |
title |
Regulatory Diversity and Functional Analysis of Two-Component Systems in Cyanobacterium Synechocystis sp. PCC 6803 by GC-MS Based Metabolomics |
title_short |
Regulatory Diversity and Functional Analysis of Two-Component Systems in Cyanobacterium Synechocystis sp. PCC 6803 by GC-MS Based Metabolomics |
title_full |
Regulatory Diversity and Functional Analysis of Two-Component Systems in Cyanobacterium Synechocystis sp. PCC 6803 by GC-MS Based Metabolomics |
title_fullStr |
Regulatory Diversity and Functional Analysis of Two-Component Systems in Cyanobacterium Synechocystis sp. PCC 6803 by GC-MS Based Metabolomics |
title_full_unstemmed |
Regulatory Diversity and Functional Analysis of Two-Component Systems in Cyanobacterium Synechocystis sp. PCC 6803 by GC-MS Based Metabolomics |
title_sort |
regulatory diversity and functional analysis of two-component systems in cyanobacterium synechocystis sp. pcc 6803 by gc-ms based metabolomics |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Microbiology |
issn |
1664-302X |
publishDate |
2020-03-01 |
description |
Two-component signal transduction systems are still poorly functionally characterized in the model cyanobacterium Synechocystis sp. PCC 6803. To address the issue, a GC-MS based comparative metabolomic analysis was conducted on a library of 44 knockout mutants for the response regulators (RRs) in Synechocystis. The metabolomic profiling analysis showed that 7 RRs mutants, namely Δslr1909, Δsll1291, Δslr6040, Δsll1330, Δslr2024, Δslr1584, and Δslr1693, were significantly different at metabolomic level, although their growth patterns are similar to the wild type under the normal autotrophic growth condition, suggesting regulatory diversity of RRs at metabolite level in Synechocystis. Additionally, a detailed metabolomic analysis coupled with RT-PCR verification led to useful clues for possible function of these 7 RRs, which were found involved in regulation of multiple aspects of cellular metabolisms in Synechocystis. Moreover, an integrative metabolomic and evolutionary analysis of all RR showed that four groups of RR genes clustered together in both metabolomic and evolutionary trees, suggesting of possible functional conservation of these RRs during the evolutionary process. Meanwhile, six groups of RRs with close evolutionary origin were found with different metabolomic profiles, suggesting possible functional changes during evolution. In contrast, more than 10 groups of RR genes with different clustering patterns in the evolutionary tree were found clustered together in metabolomics-based tree, suggesting possible functional convergences during the evolution. This study provided a metabolomic view of RR function, and the most needed functional clues for further characterization of these regulatory proteins in Synechocystis. |
topic |
Synechocystis GC-MS metabolomics two-component signal transduction systems response regulators |
url |
https://www.frontiersin.org/article/10.3389/fmicb.2020.00403/full |
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