Bioinformatics based structural characterization of glucose dehydrogenase (gdh) gene and growth promoting activity of Leclercia sp. QAU-66

• Main Authors: Muhammad Naveed, Iftikhar Ahmed, Nauman Khalid, Abdul Samad Mumtaz
• Format: Article
• Language: English
• Published: Sociedade Brasileira de Microbiologia 2014-06-01
• Series: Brazilian Journal of Microbiology
• Subjects: glucose dehydrogenase; pyrroloquinoline quinone; Leclercia sp; PGPR; bioinformatics
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200031&lng=en&tlng=en

Glucose dehydrogenase (GDH; EC 1.1. 5.2) is the member of quinoproteins group that use the redox cofactor pyrroloquinoline quinoine, calcium ions and glucose as substrate for its activity. In present study, Leclercia sp. QAU-66, isolated from rhizosphere of Vigna mungo, was characterized for phosphate solubilization and the role of GDH in plant growth promotion of Phaseolus vulgaris. The strain QAU-66 had ability to solubilize phosphorus and significantly (p < 0.05) promoted the shoot and root lengths of Phaseolus vulgaris. The structural determination of GDH protein was carried out using bioinformatics tools like Pfam, InterProScan, I-TASSER and COFACTOR. These tools predicted the structural based functional homology of pyrroloquinoline quinone domains in GDH. GDH of Leclercia sp. QAU-66 is one of the main factor that involved in plant growth promotion and provides a solid background for further research in plant growth promoting activities.