Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001
Partial 1D gene characterization was used to study phylogenetic relationships between 17 serotype O foot-and-mouth disease (FMD) viruses in Ethiopia as well as with other O-type isolates from Eritrea, Kenya, South and West Africa, the Middle East, Asia and South America. A homologous region of 4...
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doaj-de5e7d702b5e4f58ac21f00315426dc32020-11-24T21:36:04ZengAOSISOnderstepoort Journal of Veterinary Research0030-24652219-06352004-11-0171212913810.4102/ojvr.v71i2.275247Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001M. SahleE.H. VenterR.M. DwarkaW. VoslooPartial 1D gene characterization was used to study phylogenetic relationships between 17 serotype O foot-and-mouth disease (FMD) viruses in Ethiopia as well as with other O-type isolates from Eritrea, Kenya, South and West Africa, the Middle East, Asia and South America. A homologous region of 495 bp corresponding to the C-terminus end of the 1D gene was used for phylogenetic analysis. This study described three lineages, viz. African/Middle East-Asia, Cathay and South American. Within lineage I, three topotypes were defined, viz. East and West Africa and the Middle East-Asia together with the South African isolate. The Ethiopian isolates clustered as part of topotype I, the East African topotype. Two clades (based on < 12% nucleotide difference) A and B were identified within the East African isolates, with clade A being further classified into three significant branches, A1 (80 % bootstrap support), A2 (89 % bootstrap support) and A3 (94 % bootstrap support). Clade B consisted of two Kenyan isolates. Within topotype I, the 17 Ethiopian isolates showed genetic heterogeneity between themselves with sequence differences ranging from 4.6-14 %. Lineage 2 and 3 could be equated to two significant topotypes, viz. Cathay and South America. Comparison of amino acid variability at the immunodominant sites between the vaccine strain (ETH/19/77) and other Ethiopian outbreak isolates revealed variations within these sites. These results encourage further work towards the reassessment of the type O vaccine strain currently being used in Ethiopia to provide protection against field variants of the virus.https://ojvr.org/index.php/ojvr/article/view/275 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
M. Sahle E.H. Venter R.M. Dwarka W. Vosloo |
spellingShingle |
M. Sahle E.H. Venter R.M. Dwarka W. Vosloo Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001 Onderstepoort Journal of Veterinary Research |
author_facet |
M. Sahle E.H. Venter R.M. Dwarka W. Vosloo |
author_sort |
M. Sahle |
title |
Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001 |
title_short |
Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001 |
title_full |
Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001 |
title_fullStr |
Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001 |
title_full_unstemmed |
Molecular epidemiology of serotype O foot-and-mouth disease virus isolated from cattle in Ethiopia between 1979-2001 |
title_sort |
molecular epidemiology of serotype o foot-and-mouth disease virus isolated from cattle in ethiopia between 1979-2001 |
publisher |
AOSIS |
series |
Onderstepoort Journal of Veterinary Research |
issn |
0030-2465 2219-0635 |
publishDate |
2004-11-01 |
description |
Partial 1D gene characterization was used to study phylogenetic relationships between 17 serotype O foot-and-mouth disease (FMD) viruses in Ethiopia as well as with other O-type isolates from Eritrea, Kenya, South and West Africa, the Middle East, Asia and South America. A homologous region of 495 bp corresponding to the C-terminus end of the 1D gene was used for phylogenetic analysis. This study described three lineages, viz. African/Middle East-Asia, Cathay and South American. Within lineage I, three topotypes were defined, viz. East and West Africa and the Middle East-Asia together with the South African isolate. The Ethiopian isolates clustered as part of topotype I, the East African topotype. Two clades (based on < 12% nucleotide difference) A and B were identified within the East African isolates, with clade A being further classified into three significant branches, A1 (80 % bootstrap support), A2 (89 % bootstrap support) and A3 (94 % bootstrap support). Clade B consisted of two Kenyan isolates. Within topotype I, the 17 Ethiopian isolates showed genetic heterogeneity between themselves with sequence differences ranging from 4.6-14 %. Lineage 2 and 3 could be equated to two significant topotypes, viz. Cathay and South America. Comparison of amino acid variability at the immunodominant sites between the vaccine strain (ETH/19/77) and other Ethiopian outbreak isolates revealed variations within these sites. These results encourage further work towards the reassessment of the type O vaccine strain currently being used in Ethiopia to provide protection against field variants of the virus. |
url |
https://ojvr.org/index.php/ojvr/article/view/275 |
work_keys_str_mv |
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