Different construction strategies affected on the physiology of Pichia pastoris strains highly expressed lipase by transcriptional analysis of key genes

Different construction strategies affected on the physiology of Pichia pastoris strains highly expressed lipase by transcriptional analysis of key genes

We demonstrated previously that expression of Rhizomucor miehei lipase (RML) in Pichia pastoris could be significantly increased by addition of gene propeptide, optimized signal peptide codons and manipulation of gene dosage. In this study, effects of various strategies on the protein synthesis and...

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Bibliographic Details
Main Authors: Jinjin Huang, Qing Wang, Wei Bu, Lingxiao Chen, Zhen Yang, Weifa Zheng, Ying Li, Jilun Li
Format: Article
Language:English
Published: Taylor & Francis Group 2019-01-01
Series:Bioengineered
Subjects:
rhizomucor miehei lipase
pichia pastoris
propeptide
signal peptide
gene dosage
protein synthesis and secretion pathway
Online Access:http://dx.doi.org/10.1080/21655979.2019.1614422

Internet

http://dx.doi.org/10.1080/21655979.2019.1614422

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