High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore

<p>Abstract</p> <p>Background</p> <p>Recent studies suggest HIV-1 inter-subtype differences in co-receptor usage. We examined the correlation between HIV-1 subtype and co-receptor usage among treatment-naïve HIV-1 subjects in Singapore. Additionally, we investigated whe...

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Main Authors: Ng Kah Ying, Chew Kuan Kiat, Kaur Palvinder, Kwan Joe Yap, Khong Wei Xin, Lin Li, Chua Arlene, Tan Mei Ting, Quinn Thomas C, Laeyendecker Oliver, Leo Yee Sin, Ng Oon Tek
Format: Article
Language:English
Published: BMC 2013-02-01
Series:BMC Infectious Diseases
Subjects:
Online Access:http://www.biomedcentral.com/1471-2334/13/90
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spelling doaj-e46e52da5aa64476a9cf6a0796bd34ec2020-11-25T03:57:43ZengBMCBMC Infectious Diseases1471-23342013-02-011319010.1186/1471-2334-13-90High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in SingaporeNg Kah YingChew Kuan KiatKaur PalvinderKwan Joe YapKhong Wei XinLin LiChua ArleneTan Mei TingQuinn Thomas CLaeyendecker OliverLeo Yee SinNg Oon Tek<p>Abstract</p> <p>Background</p> <p>Recent studies suggest HIV-1 inter-subtype differences in co-receptor usage. We examined the correlation between HIV-1 subtype and co-receptor usage among treatment-naïve HIV-1 subjects in Singapore. Additionally, we investigated whether the subtype co-receptor association was influenced by stage of infection.</p> <p>Methods</p> <p>V3 sequences of HIV-1 envelope protein gp120 were obtained from 110 HIV treatment-naïve patients and genotypic co-receptor tropism determination was performed using Geno2pheno. Two false-positive rate (FPR) cut-offs, 10% and 5.75% were selected for tropism testing.</p> <p>Results</p> <p>Subtype assignment of viral strains from 110 HIV-infected individuals based on partial sequencing of HIV-1 <it>pol, gp120</it> and <it>gp41</it> were as follows: 27 subtype B, 64 CRF01_AE, 10 CRF51_01B, and 9 other subtypes. At FPR=10%, 10 (100%) CRF51_01B-infected subjects and 26 (40.6%) CRF01_AE-infected subjects had CXCR4-using virus, compared to 7 (25.9%) subtype B subjects and 1 (11.1%) CRF33_01B-infected subject (<it>P</it> < 0.001). At FPR=5.75%, 10 (100%) CRF51_01B-infected subjects and 20 (31.3%) CRF01_AE-infected subjects had CXCR4-using virus, compared to 4 (14.8%) subtype B and 1 (11.1%) CRF33_01B-infected subjects (<it>P</it> < 0.001). Among those with evidence of seroconversion within 2 years prior to study enrolment, 100% of CRF51_01B-infected subjects had CXCR4-using virus, independent of Geno2pheno FPR.</p> <p>Conclusion</p> <p>CRF51_01B and CRF01_AE-infected individuals have higher prevalence of CXCR4-usage compared to subtype B infected individuals. Further studies examining these differences could help optimise the use of CCR5-antagonist in populations with these subtypes, and increase our understanding of HIV-1 biology.</p> http://www.biomedcentral.com/1471-2334/13/90CXCR4 usageHIV-1treatment-naïve
collection DOAJ
language English
format Article
sources DOAJ
author Ng Kah Ying
Chew Kuan Kiat
Kaur Palvinder
Kwan Joe Yap
Khong Wei Xin
Lin Li
Chua Arlene
Tan Mei Ting
Quinn Thomas C
Laeyendecker Oliver
Leo Yee Sin
Ng Oon Tek
spellingShingle Ng Kah Ying
Chew Kuan Kiat
Kaur Palvinder
Kwan Joe Yap
Khong Wei Xin
Lin Li
Chua Arlene
Tan Mei Ting
Quinn Thomas C
Laeyendecker Oliver
Leo Yee Sin
Ng Oon Tek
High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore
BMC Infectious Diseases
CXCR4 usage
HIV-1
treatment-naïve
author_facet Ng Kah Ying
Chew Kuan Kiat
Kaur Palvinder
Kwan Joe Yap
Khong Wei Xin
Lin Li
Chua Arlene
Tan Mei Ting
Quinn Thomas C
Laeyendecker Oliver
Leo Yee Sin
Ng Oon Tek
author_sort Ng Kah Ying
title High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore
title_short High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore
title_full High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore
title_fullStr High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore
title_full_unstemmed High prevalence of CXCR4 usage among treatment-naive CRF01_AE and CRF51_01B-infected HIV-1 subjects in Singapore
title_sort high prevalence of cxcr4 usage among treatment-naive crf01_ae and crf51_01b-infected hiv-1 subjects in singapore
publisher BMC
series BMC Infectious Diseases
issn 1471-2334
publishDate 2013-02-01
description <p>Abstract</p> <p>Background</p> <p>Recent studies suggest HIV-1 inter-subtype differences in co-receptor usage. We examined the correlation between HIV-1 subtype and co-receptor usage among treatment-naïve HIV-1 subjects in Singapore. Additionally, we investigated whether the subtype co-receptor association was influenced by stage of infection.</p> <p>Methods</p> <p>V3 sequences of HIV-1 envelope protein gp120 were obtained from 110 HIV treatment-naïve patients and genotypic co-receptor tropism determination was performed using Geno2pheno. Two false-positive rate (FPR) cut-offs, 10% and 5.75% were selected for tropism testing.</p> <p>Results</p> <p>Subtype assignment of viral strains from 110 HIV-infected individuals based on partial sequencing of HIV-1 <it>pol, gp120</it> and <it>gp41</it> were as follows: 27 subtype B, 64 CRF01_AE, 10 CRF51_01B, and 9 other subtypes. At FPR=10%, 10 (100%) CRF51_01B-infected subjects and 26 (40.6%) CRF01_AE-infected subjects had CXCR4-using virus, compared to 7 (25.9%) subtype B subjects and 1 (11.1%) CRF33_01B-infected subject (<it>P</it> < 0.001). At FPR=5.75%, 10 (100%) CRF51_01B-infected subjects and 20 (31.3%) CRF01_AE-infected subjects had CXCR4-using virus, compared to 4 (14.8%) subtype B and 1 (11.1%) CRF33_01B-infected subjects (<it>P</it> < 0.001). Among those with evidence of seroconversion within 2 years prior to study enrolment, 100% of CRF51_01B-infected subjects had CXCR4-using virus, independent of Geno2pheno FPR.</p> <p>Conclusion</p> <p>CRF51_01B and CRF01_AE-infected individuals have higher prevalence of CXCR4-usage compared to subtype B infected individuals. Further studies examining these differences could help optimise the use of CCR5-antagonist in populations with these subtypes, and increase our understanding of HIV-1 biology.</p>
topic CXCR4 usage
HIV-1
treatment-naïve
url http://www.biomedcentral.com/1471-2334/13/90
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