Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes
Nutrient and organic matter concentration, microbial biomass and activities were studied at the surface microlayers (SML) and subsurface waters (SSW) in two small forest lakes of different water colour. The SML in polyhumic lake is more enriched with dissolved inorganic nitro­gen (0.141 mg l...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Exeley Inc.
2017-03-01
|
Series: | Polish Journal of Microbiology |
Subjects: | |
Online Access: | https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/1/pdf/10.5604_17331331.1234995.pdf |
id |
doaj-e496cd2ad34b4731896d01807ef711f7 |
---|---|
record_format |
Article |
spelling |
doaj-e496cd2ad34b4731896d01807ef711f72020-11-24T21:38:53ZengExeley Inc.Polish Journal of Microbiology1733-13312544-46462017-03-0166110.5604/17331331.1234995Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic LakesIwona Kostrzewska-Szlakowska0Bartosz Kiersztyn1Faculty of Biology, University of Warsaw, Warszawa, PolandDepartment of Microbial Ecology and Environmental Biotechnology, Institute of Botany, University of Warsaw; Biological and Chemical Research Centre, Warszawa, PolandNutrient and organic matter concentration, microbial biomass and activities were studied at the surface microlayers (SML) and subsurface waters (SSW) in two small forest lakes of different water colour. The SML in polyhumic lake is more enriched with dissolved inorganic nitro­gen (0.141 mg l–1) than that of oligohumic lake (0.124 mg l–1), the former also contains higher levels of total nitrogen (2.66 mg l–1). Higher activities of lipase (Vmax 2290 nmol l–1 h–1 in oligo- and 6098 in polyhumic) and glucosidase (Vmax 41 nmol l–1 h–1 in oligo- and 49 in polyhumic) were in the SMLs in both lakes. Phosphatase activity was higher in the oligohumic SML than in SSW (Vmax 632 vs. 339 nmol l–1 h–1)while in polyhumic lake was higher in SSW (Vmax 2258 nmol l–1 h–1 vs. 1908 nmol l–1 h–1). Aminopeptidase activity in the SSW in both lakes was higher than in SMLs (Vmax 2117 in oligo- and 1213 nmol l–1 h–1 in polyhumic). It seems that solar radiation does inhibit neuston micro­bial community as a whole because secondary production and the share of active bacteria in total bacteria number were higher in SSW. However, in the oligohumic lake the abundance of bacteria in the SML was always higher than in the SSW (4.07 vs. 2.69 × 106 cells ml–1) while in the polyhumic lake was roughly equal (4.48 vs. 4.33 × 106 cells ml–1) in both layers. Results may also suggest that surface communi­ties are not supplemented by immigration from bulk communities. The SML of humic lakes may act as important sinks for allochthonous nutrient resources and may then generate considerable energy pools for microbial food webs.https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/1/pdf/10.5604_17331331.1234995.pdfdystrophic lakesenzymatic activity in lakesneustonbacteria in surface microlayer |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Iwona Kostrzewska-Szlakowska Bartosz Kiersztyn |
spellingShingle |
Iwona Kostrzewska-Szlakowska Bartosz Kiersztyn Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes Polish Journal of Microbiology dystrophic lakes enzymatic activity in lakes neuston bacteria in surface microlayer |
author_facet |
Iwona Kostrzewska-Szlakowska Bartosz Kiersztyn |
author_sort |
Iwona Kostrzewska-Szlakowska |
title |
Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes |
title_short |
Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes |
title_full |
Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes |
title_fullStr |
Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes |
title_full_unstemmed |
Microbial Biomass and Enzymatic Activity of the Surface Microlayer and Subsurface Water in Two Dystrophic Lakes |
title_sort |
microbial biomass and enzymatic activity of the surface microlayer and subsurface water in two dystrophic lakes |
publisher |
Exeley Inc. |
series |
Polish Journal of Microbiology |
issn |
1733-1331 2544-4646 |
publishDate |
2017-03-01 |
description |
Nutrient and organic matter concentration, microbial biomass and activities were studied at the surface microlayers (SML) and subsurface waters (SSW) in two small forest lakes of different water colour. The SML in polyhumic lake is more enriched with dissolved inorganic nitro­gen (0.141 mg l–1) than that of oligohumic lake (0.124 mg l–1), the former also contains higher levels of total nitrogen (2.66 mg l–1). Higher activities of lipase (Vmax 2290 nmol l–1 h–1 in oligo- and 6098 in polyhumic) and glucosidase (Vmax 41 nmol l–1 h–1 in oligo- and 49 in polyhumic) were in the SMLs in both lakes. Phosphatase activity was higher in the oligohumic SML than in SSW (Vmax 632 vs. 339 nmol l–1 h–1)while in polyhumic lake was higher in SSW (Vmax 2258 nmol l–1 h–1 vs. 1908 nmol l–1 h–1). Aminopeptidase activity in the SSW in both lakes was higher than in SMLs (Vmax 2117 in oligo- and 1213 nmol l–1 h–1 in polyhumic). It seems that solar radiation does inhibit neuston micro­bial community as a whole because secondary production and the share of active bacteria in total bacteria number were higher in SSW. However, in the oligohumic lake the abundance of bacteria in the SML was always higher than in the SSW (4.07 vs. 2.69 × 106 cells ml–1) while in the polyhumic lake was roughly equal (4.48 vs. 4.33 × 106 cells ml–1) in both layers. Results may also suggest that surface communi­ties are not supplemented by immigration from bulk communities. The SML of humic lakes may act as important sinks for allochthonous nutrient resources and may then generate considerable energy pools for microbial food webs. |
topic |
dystrophic lakes enzymatic activity in lakes neuston bacteria in surface microlayer |
url |
https://www.exeley.com/exeley/journals/polish_journal_of_microbiology/66/1/pdf/10.5604_17331331.1234995.pdf |
work_keys_str_mv |
AT iwonakostrzewskaszlakowska microbialbiomassandenzymaticactivityofthesurfacemicrolayerandsubsurfacewaterintwodystrophiclakes AT bartoszkiersztyn microbialbiomassandenzymaticactivityofthesurfacemicrolayerandsubsurfacewaterintwodystrophiclakes |
_version_ |
1725933909398519808 |