Microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral DNA

Portable and simple analytical devices based on microfluidics with chemiluminescence detection are particularly attractive for point-of-care applications, offering high detectability and specificity in a simple and miniaturized analytical format. Particularly relevant for infectious disease diagnosi...

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Main Authors: M. Zangheri, M. Mirasoli, A. Nascetti, D. Caputo, F. Bonvicini, G. Gallinella, G. de Cesare, A. Roda
Format: Article
Language:English
Published: Elsevier 2016-03-01
Series:Sensing and Bio-Sensing Research
Online Access:http://www.sciencedirect.com/science/article/pii/S2214180416300071
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spelling doaj-e5280535692f48feacaa4535706f31822020-11-24T22:33:49ZengElsevierSensing and Bio-Sensing Research2214-18042016-03-017127132Microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral DNAM. Zangheri0M. Mirasoli1A. Nascetti2D. Caputo3F. Bonvicini4G. Gallinella5G. de Cesare6A. Roda7Department of Chemistry “Giacomo Ciamician”, University of Bologna, ItalyDepartment of Chemistry “Giacomo Ciamician”, University of Bologna, Italy; Corresponding author.Aerospace Engineering School, Sapienza University of Rome, ItalyDepartment of Information, Electronics and Communication Engineering, Sapienza University of Rome, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, ItalyDepartment of Pharmacy and Biotechnology, University of Bologna, ItalyDepartment of Information, Electronics and Communication Engineering, Sapienza University of Rome, ItalyDepartment of Chemistry “Giacomo Ciamician”, University of Bologna, ItalyPortable and simple analytical devices based on microfluidics with chemiluminescence detection are particularly attractive for point-of-care applications, offering high detectability and specificity in a simple and miniaturized analytical format. Particularly relevant for infectious disease diagnosis is the ability to sensitively and specifically detect target nucleic acid sequences in biological fluids. To reach the goal of real-life applications for such devices, however, several technological challenges related to full device integration are still to be solved, one key aspect regarding on-chip integration of the chemiluminescence signal detection device. Nowadays, the most promising approach is on-chip integration of thin-film photosensors.We recently proposed a portable cartridge with microwells aligned with an array of hydrogenated amorphous silicon (a-Si:H) photosensors, reaching attomole level limits of detection for different chemiluminescence model reactions. Herein, we explore its applicability and performance for multiplex and quantitative detection of viral DNA. In particular, the cartridge was modified to accommodate microfluidic channels and, upon immobilization of three oligonucleotide probes in different positions along each channel, each specific for a genotype of Parvovirus B19, viral nucleic acid sequences were captured and detected.With this system, taking advantage of oligoprobes specificity, chemiluminescence detectability, and photosensor sensitivity, accurate quantification of target analytes down to 70 pmol L−1 was obtained for each B19 DNA genotype, with high specificity and multiplexing ability. Results confirm the good detection capabilities and assay applicability of the proposed system, prompting the development of innovative portable analytical devices with enhanced sensitivity and multiplexed capabilities. Keywords: Integrated analytical system, Amorphous silicon photosensors, Chemiluminescence, Nucleic acids detection, Oligonucleotide array, Point-of-carehttp://www.sciencedirect.com/science/article/pii/S2214180416300071
collection DOAJ
language English
format Article
sources DOAJ
author M. Zangheri
M. Mirasoli
A. Nascetti
D. Caputo
F. Bonvicini
G. Gallinella
G. de Cesare
A. Roda
spellingShingle M. Zangheri
M. Mirasoli
A. Nascetti
D. Caputo
F. Bonvicini
G. Gallinella
G. de Cesare
A. Roda
Microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral DNA
Sensing and Bio-Sensing Research
author_facet M. Zangheri
M. Mirasoli
A. Nascetti
D. Caputo
F. Bonvicini
G. Gallinella
G. de Cesare
A. Roda
author_sort M. Zangheri
title Microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral DNA
title_short Microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral DNA
title_full Microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral DNA
title_fullStr Microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral DNA
title_full_unstemmed Microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral DNA
title_sort microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral dna
publisher Elsevier
series Sensing and Bio-Sensing Research
issn 2214-1804
publishDate 2016-03-01
description Portable and simple analytical devices based on microfluidics with chemiluminescence detection are particularly attractive for point-of-care applications, offering high detectability and specificity in a simple and miniaturized analytical format. Particularly relevant for infectious disease diagnosis is the ability to sensitively and specifically detect target nucleic acid sequences in biological fluids. To reach the goal of real-life applications for such devices, however, several technological challenges related to full device integration are still to be solved, one key aspect regarding on-chip integration of the chemiluminescence signal detection device. Nowadays, the most promising approach is on-chip integration of thin-film photosensors.We recently proposed a portable cartridge with microwells aligned with an array of hydrogenated amorphous silicon (a-Si:H) photosensors, reaching attomole level limits of detection for different chemiluminescence model reactions. Herein, we explore its applicability and performance for multiplex and quantitative detection of viral DNA. In particular, the cartridge was modified to accommodate microfluidic channels and, upon immobilization of three oligonucleotide probes in different positions along each channel, each specific for a genotype of Parvovirus B19, viral nucleic acid sequences were captured and detected.With this system, taking advantage of oligoprobes specificity, chemiluminescence detectability, and photosensor sensitivity, accurate quantification of target analytes down to 70 pmol L−1 was obtained for each B19 DNA genotype, with high specificity and multiplexing ability. Results confirm the good detection capabilities and assay applicability of the proposed system, prompting the development of innovative portable analytical devices with enhanced sensitivity and multiplexed capabilities. Keywords: Integrated analytical system, Amorphous silicon photosensors, Chemiluminescence, Nucleic acids detection, Oligonucleotide array, Point-of-care
url http://www.sciencedirect.com/science/article/pii/S2214180416300071
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