Gestational zinc deficiency impairs humoral and cellular immune responses to hepatitis B vaccination in offspring mice.
BACKGROUND: Gestational zinc deficiency has been confirmed to impair the infant immune function. However, knowledge about effects of maternal mild zinc deficiency during pregnancy on hepatitis B vaccine responsiveness in offspring is limited. In this report, we aimed to examine how maternal zinc def...
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doaj-e5e7b503fd38496fad80c8b727d4b1352020-11-24T21:44:32ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0189e7346110.1371/journal.pone.0073461Gestational zinc deficiency impairs humoral and cellular immune responses to hepatitis B vaccination in offspring mice.Ning ZhaoXuelian WangYing ZhangQiuhong GuFen HuangWei ZhengZhiwei LiBACKGROUND: Gestational zinc deficiency has been confirmed to impair the infant immune function. However, knowledge about effects of maternal mild zinc deficiency during pregnancy on hepatitis B vaccine responsiveness in offspring is limited. In this report, we aimed to examine how maternal zinc deficiency during pregnancy influences humoral and cellular immune responses to hepatitis B vaccination in offspring of BALB/c mice. METHODOLOGY/PRINCIPAL FINDINGS: From day 1 of pregnancy upon delivery, maternal mice were given a standard diet (30 mg/kg/day zinc), zinc deficient diet (8 mg/kg/day zinc), or combination of zinc deficient diet (8 mg/kg/day zinc in the first 2 weeks of gestation) and zinc supplement diet (150 mg/kg/day zinc for the last week of pregnancy), respectively. Newborn pups of these maternal mice were immunized with hepatitis B vaccine at postnatal weeks 0, 2 and 4. Then, splenocytes and blood samples from the offspring mice were harvested for detection of serum zinc concentrations, humoral and cell-mediated immune responses, expression of cytokines using ELISA, CCK-8 and flow cytometric analysis. Results from the present study demonstrated that gestational zinc deficiency inhibited antibody responses, and decreased the proliferative capacity of T cells in offsprings immunized with hepatitis B vaccine. Additionally, HBsAg-specific cytokines analysis revealed that gestational zinc deficiency could inhibit secretion of IFN-γ from splenocytes, and decrease IFN-γ expression of CD4(+) and CD8(+) T cells. CONCLUSIONS/SIGNIFICANCE: Gestational zinc deficiency can weaken the humoral and cell-mediated immune responses to hepatitis B vaccine via decreasing B cell counts and hepatitis B virus-specific immunoglobulin G production, as well as reducing T cell proliferation, CD4(+)/CD8(+) T cell ratio, and Th1-type immune responses.http://europepmc.org/articles/PMC3775768?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Ning Zhao Xuelian Wang Ying Zhang Qiuhong Gu Fen Huang Wei Zheng Zhiwei Li |
spellingShingle |
Ning Zhao Xuelian Wang Ying Zhang Qiuhong Gu Fen Huang Wei Zheng Zhiwei Li Gestational zinc deficiency impairs humoral and cellular immune responses to hepatitis B vaccination in offspring mice. PLoS ONE |
author_facet |
Ning Zhao Xuelian Wang Ying Zhang Qiuhong Gu Fen Huang Wei Zheng Zhiwei Li |
author_sort |
Ning Zhao |
title |
Gestational zinc deficiency impairs humoral and cellular immune responses to hepatitis B vaccination in offspring mice. |
title_short |
Gestational zinc deficiency impairs humoral and cellular immune responses to hepatitis B vaccination in offspring mice. |
title_full |
Gestational zinc deficiency impairs humoral and cellular immune responses to hepatitis B vaccination in offspring mice. |
title_fullStr |
Gestational zinc deficiency impairs humoral and cellular immune responses to hepatitis B vaccination in offspring mice. |
title_full_unstemmed |
Gestational zinc deficiency impairs humoral and cellular immune responses to hepatitis B vaccination in offspring mice. |
title_sort |
gestational zinc deficiency impairs humoral and cellular immune responses to hepatitis b vaccination in offspring mice. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS ONE |
issn |
1932-6203 |
publishDate |
2013-01-01 |
description |
BACKGROUND: Gestational zinc deficiency has been confirmed to impair the infant immune function. However, knowledge about effects of maternal mild zinc deficiency during pregnancy on hepatitis B vaccine responsiveness in offspring is limited. In this report, we aimed to examine how maternal zinc deficiency during pregnancy influences humoral and cellular immune responses to hepatitis B vaccination in offspring of BALB/c mice. METHODOLOGY/PRINCIPAL FINDINGS: From day 1 of pregnancy upon delivery, maternal mice were given a standard diet (30 mg/kg/day zinc), zinc deficient diet (8 mg/kg/day zinc), or combination of zinc deficient diet (8 mg/kg/day zinc in the first 2 weeks of gestation) and zinc supplement diet (150 mg/kg/day zinc for the last week of pregnancy), respectively. Newborn pups of these maternal mice were immunized with hepatitis B vaccine at postnatal weeks 0, 2 and 4. Then, splenocytes and blood samples from the offspring mice were harvested for detection of serum zinc concentrations, humoral and cell-mediated immune responses, expression of cytokines using ELISA, CCK-8 and flow cytometric analysis. Results from the present study demonstrated that gestational zinc deficiency inhibited antibody responses, and decreased the proliferative capacity of T cells in offsprings immunized with hepatitis B vaccine. Additionally, HBsAg-specific cytokines analysis revealed that gestational zinc deficiency could inhibit secretion of IFN-γ from splenocytes, and decrease IFN-γ expression of CD4(+) and CD8(+) T cells. CONCLUSIONS/SIGNIFICANCE: Gestational zinc deficiency can weaken the humoral and cell-mediated immune responses to hepatitis B vaccine via decreasing B cell counts and hepatitis B virus-specific immunoglobulin G production, as well as reducing T cell proliferation, CD4(+)/CD8(+) T cell ratio, and Th1-type immune responses. |
url |
http://europepmc.org/articles/PMC3775768?pdf=render |
work_keys_str_mv |
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