Propagation of Salmonella enterica serovar typhimurium in embryonated chicken egg

• Main Authors: Ammar Al-AAlim, Sumaya Aldabbagh, Haiffa Alkazaly, Mozahim Al-Attar
• Format: Article
• Language: Arabic
• Published: University of Mosul, College of Veterinary Medicine 2019-09-01
• Series: Iraqi Journal of Veterinary Sciences
• Subjects: salmonella enterica; propagation; embryonated chikcen egg; starling birds
• Online Access: https://vetmedmosul.com/article_163171_d6926a2037f667b8343a0a57f34d16ef.pdf
• ISSN: 1607-3894; 2071-1255

The study included the injection of <em>Salmonella enterica </em>serovar<em> typhimurium</em>isolated from starlings bird in embryonated chicken eggs. The eggs were divided into eight groups, each group contain 6 eggs. The G1 and G2 groups were injected with the sterile normal saline solution in the choriaollantoic membrane (CAM) and yolk sac as negative control. The group G3 and G4 injected by bacterial suspension at a concentration of 10⁴ cfu/ml in the CAM and yolk sac while the G5 and G6 injected with bacterial concentration 10⁶ cfu/ml in the CAM and yolk sac respectively. Finally, the G7 and G8 groups were injected with 10⁸ cfu/ml of bacterial suspension in the CAM and yolk sac respectively. The results showed that the highest percentage of death in eggs embryos was 100% in the sixth group after 96 hours of injection. There was also a significant increase in the number of bacteria in correlation with time of incubation. The highest rate of bacterial isolate was 8,19log10, 8,26log10 after 96 and 144 hours in the sixth group, while the highest number of bacterial isolates was 7.04log10 and 6.31log10 in the third and fourth groups after 48 and 96 hours of injection respectively. The results of the statistical analysis showed a significant difference in the number of bacterial isolate after 24 hours of injection in both concentrations compared to other incubation times. A significantrelationship was also found between the amount of the dose used and the bacterial disease. this study concluded that <em>Salmonella enterica </em>serovar<em> typhimurium</em> isolated from starlings can cause pathological changes and effect on hatchery percentage in embryonated chicken eggs.