Modifying inter-cistronic sequence significantly enhances IRES dependent second gene expression in bicistronic vector: Construction of optimised cassette for gene therapy of familial hypercholesterolemia
Internal ribosome entry site (IRES) sequences have become a valuable tool in the construction of gene transfer and therapeutic vectors for multi-cistronic gene expression from a single mRNA transcript. The optimal conditions for effective use of this sequence to construct a functional expression vec...
Main Authors: | Faisal A. Al-Allaf, Zainularifeen Abduljaleel, Mohammad Athar, Mohiuddin M. Taher, Wajahatullah Khan, Huseyin Mehmet, Mukaddes Colakogullari, Sophia Apostolidou, Brian Bigger, Simon Waddington, Charles Coutelle, Michael Themis, Mohammed N. Al-Ahdal, Futwan A. Al-Mohanna, Zuhair N. Al-Hassnan, Abdellatif Bouazzaoui |
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Format: | Article |
Language: | English |
Published: |
KeAi Communications Co., Ltd.
2019-03-01
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Series: | Non-coding RNA Research |
Online Access: | http://www.sciencedirect.com/science/article/pii/S246805401830088X |
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