Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>

Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from <i>Fucus vesiculosus</i> using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol...

Full description

Bibliographic Details
Main Authors: Sónia J. Amarante, Marcelo D. Catarino, Catarina Marçal, Artur M. S. Silva, Rita Ferreira, Susana M. Cardoso
Format: Article
Language:English
Published: MDPI AG 2020-11-01
Series:Marine Drugs
Subjects:
Online Access:https://www.mdpi.com/1660-3397/18/11/559
id doaj-e7c0cacac3694850bae3fce70a88d3a9
record_format Article
spelling doaj-e7c0cacac3694850bae3fce70a88d3a92020-11-25T04:09:17ZengMDPI AGMarine Drugs1660-33972020-11-011855955910.3390/md18110559Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>Sónia J. Amarante0Marcelo D. Catarino1Catarina Marçal2Artur M. S. Silva3Rita Ferreira4Susana M. Cardoso5LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalMicrowave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from <i>Fucus vesiculosus</i> using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (<i>v/v</i>), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DW<sub>extract</sub>), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS<sup>•+</sup> (IC<sub>50</sub> of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC<sub>50</sub> of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose.https://www.mdpi.com/1660-3397/18/11/559brown seaweedsphlorotanninsmicrowave-assisted extractionresponse surface methodologyantioxidantantiradical activity
collection DOAJ
language English
format Article
sources DOAJ
author Sónia J. Amarante
Marcelo D. Catarino
Catarina Marçal
Artur M. S. Silva
Rita Ferreira
Susana M. Cardoso
spellingShingle Sónia J. Amarante
Marcelo D. Catarino
Catarina Marçal
Artur M. S. Silva
Rita Ferreira
Susana M. Cardoso
Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>
Marine Drugs
brown seaweeds
phlorotannins
microwave-assisted extraction
response surface methodology
antioxidant
antiradical activity
author_facet Sónia J. Amarante
Marcelo D. Catarino
Catarina Marçal
Artur M. S. Silva
Rita Ferreira
Susana M. Cardoso
author_sort Sónia J. Amarante
title Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>
title_short Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>
title_full Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>
title_fullStr Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>
title_full_unstemmed Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>
title_sort microwave-assisted extraction of phlorotannins from <i>fucus vesiculosus</i>
publisher MDPI AG
series Marine Drugs
issn 1660-3397
publishDate 2020-11-01
description Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from <i>Fucus vesiculosus</i> using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (<i>v/v</i>), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DW<sub>extract</sub>), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS<sup>•+</sup> (IC<sub>50</sub> of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC<sub>50</sub> of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose.
topic brown seaweeds
phlorotannins
microwave-assisted extraction
response surface methodology
antioxidant
antiradical activity
url https://www.mdpi.com/1660-3397/18/11/559
work_keys_str_mv AT soniajamarante microwaveassistedextractionofphlorotanninsfromifucusvesiculosusi
AT marcelodcatarino microwaveassistedextractionofphlorotanninsfromifucusvesiculosusi
AT catarinamarcal microwaveassistedextractionofphlorotanninsfromifucusvesiculosusi
AT arturmssilva microwaveassistedextractionofphlorotanninsfromifucusvesiculosusi
AT ritaferreira microwaveassistedextractionofphlorotanninsfromifucusvesiculosusi
AT susanamcardoso microwaveassistedextractionofphlorotanninsfromifucusvesiculosusi
_version_ 1724422542783938560