Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>
Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from <i>Fucus vesiculosus</i> using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol...
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doaj-e7c0cacac3694850bae3fce70a88d3a92020-11-25T04:09:17ZengMDPI AGMarine Drugs1660-33972020-11-011855955910.3390/md18110559Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i>Sónia J. Amarante0Marcelo D. Catarino1Catarina Marçal2Artur M. S. Silva3Rita Ferreira4Susana M. Cardoso5LAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalLAQV-REQUIMTE, Department of Chemistry, University of Aveiro, 3810-193 Aveiro, PortugalMicrowave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from <i>Fucus vesiculosus</i> using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (<i>v/v</i>), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DW<sub>extract</sub>), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS<sup>•+</sup> (IC<sub>50</sub> of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC<sub>50</sub> of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose.https://www.mdpi.com/1660-3397/18/11/559brown seaweedsphlorotanninsmicrowave-assisted extractionresponse surface methodologyantioxidantantiradical activity |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Sónia J. Amarante Marcelo D. Catarino Catarina Marçal Artur M. S. Silva Rita Ferreira Susana M. Cardoso |
spellingShingle |
Sónia J. Amarante Marcelo D. Catarino Catarina Marçal Artur M. S. Silva Rita Ferreira Susana M. Cardoso Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> Marine Drugs brown seaweeds phlorotannins microwave-assisted extraction response surface methodology antioxidant antiradical activity |
author_facet |
Sónia J. Amarante Marcelo D. Catarino Catarina Marçal Artur M. S. Silva Rita Ferreira Susana M. Cardoso |
author_sort |
Sónia J. Amarante |
title |
Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> |
title_short |
Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> |
title_full |
Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> |
title_fullStr |
Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> |
title_full_unstemmed |
Microwave-Assisted Extraction of Phlorotannins from <i>Fucus vesiculosus</i> |
title_sort |
microwave-assisted extraction of phlorotannins from <i>fucus vesiculosus</i> |
publisher |
MDPI AG |
series |
Marine Drugs |
issn |
1660-3397 |
publishDate |
2020-11-01 |
description |
Microwave-assisted extraction (MAE) was carried out to maximize the extraction of phlorotannins from <i>Fucus vesiculosus</i> using a hydroethanolic mixture as a solvent, as an alternative to the conventional method with a hydroacetonic mixture. Optimal MAE conditions were set as ethanol concentration of 57% (<i>v/v</i>), temperature of 75 °C, and time of 5 min, which allowed a similar recovery of phlorotannins from the macroalgae compared to the conventional extraction. While the phlorotannins richness of the conventional extract was slightly superior to that of MAE (11.1 ± 1.3 vs. 9.8 ± 1.8 mg PGE/g DW<sub>extract</sub>), both extracts presented identical phlorotannins constituents, which included, among others, tetrafucol, pentafucol, hexafucol, and heptafucol structures. In addition, MAE showed a moderate capacity to scavenge ABTS<sup>•+</sup> (IC<sub>50</sub> of 96.0 ± 3.4 µg/mL) and to inhibit the activity of xanthine oxidase (IC<sub>50</sub> of 23.1 ± 3.4 µg/mL) and a superior ability to control the activity of the key metabolic enzyme α-glucosidase compared to the pharmaceutical drug acarbose. |
topic |
brown seaweeds phlorotannins microwave-assisted extraction response surface methodology antioxidant antiradical activity |
url |
https://www.mdpi.com/1660-3397/18/11/559 |
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