A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249
We have investigated the quorum sensing control in Aeromonas veronii MTCC 3249, originally isolated as A. culicicola from the midgut of Culex quinquefasciatus. Based on biosensor assays, the bacterium showed constant production of multiple acyl-homoserine lactones (AHLs) with increasing cell-density...
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doaj-e980bda445dd45fda5d96984c756270e2020-11-25T01:41:58ZengMDPI AGSensors1424-82202012-03-011243814383010.3390/s120403814A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249Yogesh S. ShouchePerunninakulath S. ParameswaranKamlesh JangidWe have investigated the quorum sensing control in Aeromonas veronii MTCC 3249, originally isolated as A. culicicola from the midgut of Culex quinquefasciatus. Based on biosensor assays, the bacterium showed constant production of multiple acyl-homoserine lactones (AHLs) with increasing cell-density. The luxRI gene homologs, acuR (A. culicicola transcriptional Regulator) and acuI (A. culicicola autoInducer) were successfully amplified by inverse-PCR. Sequence analysis indicated acuRI were divergent from all known quorum sensing gene homologs in Aeromonas. Two localized regions in the C-terminal autoinducer binding domain of acuR showed indels suggesting variations in autoinducer specificity. Further, only a single copy of the quorum sensing genes was detected, suggesting a tight regulation of mechanisms under its control. Chromatography and further chemical analysis identified two AHLs in the culture supernatant: 6-carboxy-HHL (homoadipyl homoserine lactone), a novel AHL, and N-tetradecanoylhomoserine lactone. The existence of a potentially variant quorum sensing system might therefore, reflect in some way the ecological strategies adopted by this bacterium in the mosquito midgut.http://www.mdpi.com/1424-8220/12/4/3814/AeromonasluxRI homologacuRacuIquorum sensingAHLmosquito midgut6-carboxy-HHL |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Yogesh S. Shouche Perunninakulath S. Parameswaran Kamlesh Jangid |
spellingShingle |
Yogesh S. Shouche Perunninakulath S. Parameswaran Kamlesh Jangid A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249 Sensors Aeromonas luxRI homolog acuR acuI quorum sensing AHL mosquito midgut 6-carboxy-HHL |
author_facet |
Yogesh S. Shouche Perunninakulath S. Parameswaran Kamlesh Jangid |
author_sort |
Yogesh S. Shouche |
title |
A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249 |
title_short |
A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249 |
title_full |
A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249 |
title_fullStr |
A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249 |
title_full_unstemmed |
A Variant Quorum Sensing System in Aeromonas veronii MTCC 3249 |
title_sort |
variant quorum sensing system in aeromonas veronii mtcc 3249 |
publisher |
MDPI AG |
series |
Sensors |
issn |
1424-8220 |
publishDate |
2012-03-01 |
description |
We have investigated the quorum sensing control in Aeromonas veronii MTCC 3249, originally isolated as A. culicicola from the midgut of Culex quinquefasciatus. Based on biosensor assays, the bacterium showed constant production of multiple acyl-homoserine lactones (AHLs) with increasing cell-density. The luxRI gene homologs, acuR (A. culicicola transcriptional Regulator) and acuI (A. culicicola autoInducer) were successfully amplified by inverse-PCR. Sequence analysis indicated acuRI were divergent from all known quorum sensing gene homologs in Aeromonas. Two localized regions in the C-terminal autoinducer binding domain of acuR showed indels suggesting variations in autoinducer specificity. Further, only a single copy of the quorum sensing genes was detected, suggesting a tight regulation of mechanisms under its control. Chromatography and further chemical analysis identified two AHLs in the culture supernatant: 6-carboxy-HHL (homoadipyl homoserine lactone), a novel AHL, and N-tetradecanoylhomoserine lactone. The existence of a potentially variant quorum sensing system might therefore, reflect in some way the ecological strategies adopted by this bacterium in the mosquito midgut. |
topic |
Aeromonas luxRI homolog acuR acuI quorum sensing AHL mosquito midgut 6-carboxy-HHL |
url |
http://www.mdpi.com/1424-8220/12/4/3814/ |
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