The Role of Saccharides in the Mechanisms of Pathogenicity of <em>Fusarium oxysporum</em> f. sp. <em>lupini</em> in Yellow Lupine (<em>Lupinus luteus</em> L.)
The primary aim of this study was to determine the relationship between soluble sugar levels (sucrose, glucose, or fructose) in yellow lupine embryo axes and the pathogenicity of the hemibiotrophic fungus <i>Fusarium oxysporum</i> f. sp. Schlecht <i>lupini</i>. The first step...
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doaj-e99c59d4ab8f4933849be004b0ae232b2020-11-25T03:57:27ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-10-01217258725810.3390/ijms21197258The Role of Saccharides in the Mechanisms of Pathogenicity of <em>Fusarium oxysporum</em> f. sp. <em>lupini</em> in Yellow Lupine (<em>Lupinus luteus</em> L.)Magda Formela-Luboińska0Dorota Remlein-Starosta1Agnieszka Waśkiewicz2Zbigniew Karolewski3Jan Bocianowski4Łukasz Stępień5Mateusz Labudda6Philippe Jeandet7Iwona Morkunas8Department of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, 60-637 Poznań, PolandDepartment of Ecology and Environmental Protection, Institute of Plant Protection—National Research Institute, Władysława Węgorka 20, 60-318 Poznań, PolandDepartment of Chemistry, Poznań University of Life Sciences, Wojska Polskiego 75, 60-625 Poznań, PolandDepartment of Phytopathology, Seed Science and Technology, Poznań University of Life Sciences, Dąbrowskiego 159, 60-594 Poznań, PolandDepartment of Mathematical and Statistical Methods, Poznań University of Life Sciences, Wojska Polskiego 28, 60-637 Poznań, PolandDepartment of Pathogen Genetics and Plant Resistance, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, PolandDepartment of Biochemistry and Microbiology, Institute of Biology, Warsaw University of Life Sciences-SGGW, Nowoursynowska 159, 02-776 Warsaw, PolandResearch Unit “Induced Resistance and Plant Bioprotection”, UPRES EA 4707, Department of Biology and Biochemistry, Faculty of Sciences, University of Reims, P.O. Box 1039, CEDEX 02, 51687 Reims, FranceDepartment of Plant Physiology, Poznań University of Life Sciences, Wołyńska 35, 60-637 Poznań, PolandThe primary aim of this study was to determine the relationship between soluble sugar levels (sucrose, glucose, or fructose) in yellow lupine embryo axes and the pathogenicity of the hemibiotrophic fungus <i>Fusarium oxysporum</i> f. sp. Schlecht <i>lupini</i>. The first step of this study was to determine the effect of exogenous saccharides on the growth and sporulation of <i>F</i>. <i>oxysporum</i>. The second one focused on estimating the levels of ergosterol as a fungal growth indicator in infected embryo axes cultured in vitro on sugar containing-medium or without it. The third aim of this study was to record the levels of the mycotoxin moniliformin as the most characteristic secondary metabolite of <i>F</i>. <i>oxysporum</i> in the infected embryo axes with the high sugar medium and without it. Additionally, morphometric measurements, i.e., the length and fresh weight of embryo axes, were done. The levels of ergosterol were the highest in infected embryo axes with a sugar deficit. At the same time, significant accumulation of the mycotoxin moniliformin was recorded in those tissues. Furthermore, it was found that the presence of sugars in water agar medium inhibited the sporulation of the pathogenic fungus <i>F</i>. <i>oxysporum</i> in relation to the control (sporulation of the pathogen on medium without sugar), the strongest inhibiting effect was observed in the case of glucose. Infection caused by <i>F</i>. <i>oxysporum</i> significantly limited the growth of embryo axes, but this effect was more visible on infected axes cultured under sugar deficiency than on the ones cultured with soluble sugars. The obtained results thus showed that high sugar levels may lead to reduced production of mycotoxins by <i>F</i>. <i>oxysporum,</i> limiting infection development and fusariosis.https://www.mdpi.com/1422-0067/21/19/7258ergosterolfungal sporulation<i>Fusarium oxysporum</i><i>Lupinus luteus</i>moniliforminmycelium growth |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Magda Formela-Luboińska Dorota Remlein-Starosta Agnieszka Waśkiewicz Zbigniew Karolewski Jan Bocianowski Łukasz Stępień Mateusz Labudda Philippe Jeandet Iwona Morkunas |
spellingShingle |
Magda Formela-Luboińska Dorota Remlein-Starosta Agnieszka Waśkiewicz Zbigniew Karolewski Jan Bocianowski Łukasz Stępień Mateusz Labudda Philippe Jeandet Iwona Morkunas The Role of Saccharides in the Mechanisms of Pathogenicity of <em>Fusarium oxysporum</em> f. sp. <em>lupini</em> in Yellow Lupine (<em>Lupinus luteus</em> L.) International Journal of Molecular Sciences ergosterol fungal sporulation <i>Fusarium oxysporum</i> <i>Lupinus luteus</i> moniliformin mycelium growth |
author_facet |
Magda Formela-Luboińska Dorota Remlein-Starosta Agnieszka Waśkiewicz Zbigniew Karolewski Jan Bocianowski Łukasz Stępień Mateusz Labudda Philippe Jeandet Iwona Morkunas |
author_sort |
Magda Formela-Luboińska |
title |
The Role of Saccharides in the Mechanisms of Pathogenicity of <em>Fusarium oxysporum</em> f. sp. <em>lupini</em> in Yellow Lupine (<em>Lupinus luteus</em> L.) |
title_short |
The Role of Saccharides in the Mechanisms of Pathogenicity of <em>Fusarium oxysporum</em> f. sp. <em>lupini</em> in Yellow Lupine (<em>Lupinus luteus</em> L.) |
title_full |
The Role of Saccharides in the Mechanisms of Pathogenicity of <em>Fusarium oxysporum</em> f. sp. <em>lupini</em> in Yellow Lupine (<em>Lupinus luteus</em> L.) |
title_fullStr |
The Role of Saccharides in the Mechanisms of Pathogenicity of <em>Fusarium oxysporum</em> f. sp. <em>lupini</em> in Yellow Lupine (<em>Lupinus luteus</em> L.) |
title_full_unstemmed |
The Role of Saccharides in the Mechanisms of Pathogenicity of <em>Fusarium oxysporum</em> f. sp. <em>lupini</em> in Yellow Lupine (<em>Lupinus luteus</em> L.) |
title_sort |
role of saccharides in the mechanisms of pathogenicity of <em>fusarium oxysporum</em> f. sp. <em>lupini</em> in yellow lupine (<em>lupinus luteus</em> l.) |
publisher |
MDPI AG |
series |
International Journal of Molecular Sciences |
issn |
1661-6596 1422-0067 |
publishDate |
2020-10-01 |
description |
The primary aim of this study was to determine the relationship between soluble sugar levels (sucrose, glucose, or fructose) in yellow lupine embryo axes and the pathogenicity of the hemibiotrophic fungus <i>Fusarium oxysporum</i> f. sp. Schlecht <i>lupini</i>. The first step of this study was to determine the effect of exogenous saccharides on the growth and sporulation of <i>F</i>. <i>oxysporum</i>. The second one focused on estimating the levels of ergosterol as a fungal growth indicator in infected embryo axes cultured in vitro on sugar containing-medium or without it. The third aim of this study was to record the levels of the mycotoxin moniliformin as the most characteristic secondary metabolite of <i>F</i>. <i>oxysporum</i> in the infected embryo axes with the high sugar medium and without it. Additionally, morphometric measurements, i.e., the length and fresh weight of embryo axes, were done. The levels of ergosterol were the highest in infected embryo axes with a sugar deficit. At the same time, significant accumulation of the mycotoxin moniliformin was recorded in those tissues. Furthermore, it was found that the presence of sugars in water agar medium inhibited the sporulation of the pathogenic fungus <i>F</i>. <i>oxysporum</i> in relation to the control (sporulation of the pathogen on medium without sugar), the strongest inhibiting effect was observed in the case of glucose. Infection caused by <i>F</i>. <i>oxysporum</i> significantly limited the growth of embryo axes, but this effect was more visible on infected axes cultured under sugar deficiency than on the ones cultured with soluble sugars. The obtained results thus showed that high sugar levels may lead to reduced production of mycotoxins by <i>F</i>. <i>oxysporum,</i> limiting infection development and fusariosis. |
topic |
ergosterol fungal sporulation <i>Fusarium oxysporum</i> <i>Lupinus luteus</i> moniliformin mycelium growth |
url |
https://www.mdpi.com/1422-0067/21/19/7258 |
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