Sparse PrP^Scaccumulation in the placentas of goats with naturally acquired scrapie

• Main Authors: Yan Huijan, Truscott Thomas C, Zhuang Dongyue, O'Rourke Katherine I, Schneider David A
• Format: Article
• Language: English
• Published: BMC 2011-02-01
• Series: BMC Veterinary Research
• Online Access: http://www.biomedcentral.com/1746-6148/7/7

<p>Abstract</p> <p>Background</p> <p>Domestic goats (<it>Capra hircus</it>) are a natural and experimental host of scrapie and bovine spongiform encephalopathy, the transmissible spongiform encephalopathies (TSE) of sheep and cattle. Goats are also susceptible to experimental infection with the agents of TSEs of deer and elk (chronic wasting disease) and humans (Creutzfeldt Jakob disease). Distribution of PrP^Sc, the abnormal prion protein, is similar in the tissues of scrapie-infected sheep and goats but no data are available on the potential shedding of the agent through the placenta, the presumed route of transmission of ovine scrapie. We describe the sparse accumulation of PrP^Scin the placentas of goats with naturally acquired classical scrapie in comparison to field cases of classical ovine scrapie.</p> <p>Results</p> <p>PrP^Scwas detected in the shed placentas from a sample of U.S. goats with naturally occurring scrapie, diagnosed by antemortem lymphoid tissue biopsy or identified as high risk progeny of infected dams. PrP^Scaccumulation patterns in the intact placentome and western blot banding was similar in the caprine and ovine samples. However, levels of PrP^Scestimated from ELISA and immunohistochemistry assays were generally lower in goats than in sheep, although wide variation was noted in both species.</p> <p>Conclusions</p> <p>PrP^Scaccumulates in the shed placentas of goats with naturally acquired scrapie. Although these levels were low in most caprine samples, the caprine placenta may contribute to prion contamination of kidding facilities and transmission to co-housed sheep or goats.</p>