Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures

Epidemiological studies show that fruit consumption may modulate bone mineral density. However, data regarding the effect of the Citrus bergamia Risso (Bergamot orange), a citrus fruit containing a high concentration of flavonoids, on bone health are still lacking. In this study, we investigated the...

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Main Authors: Arturo Pujia, Cristina Russo, Samantha Maurotti, Roberta Pujia, Vincenzo Mollace, Stefano Romeo, Tiziana Montalcini
Format: Article
Language:English
Published: MDPI AG 2018-09-01
Series:Nutrients
Subjects:
Online Access:http://www.mdpi.com/2072-6643/10/9/1305
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spelling doaj-eb5f1b4349a34aa5aa67ef8bf18fe1292020-11-24T21:08:51ZengMDPI AGNutrients2072-66432018-09-01109130510.3390/nu10091305nu10091305Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell CulturesArturo Pujia0Cristina Russo1Samantha Maurotti2Roberta Pujia3Vincenzo Mollace4Stefano Romeo5Tiziana Montalcini6Department of Medical and Surgical Science, University Magna Grecia, 88100 Catanzaro, ItalyDepartment of Medical and Surgical Science, University Magna Grecia, 88100 Catanzaro, ItalyDepartment of Medical and Surgical Science, University Magna Grecia, 88100 Catanzaro, ItalyDepartment of Health Science, University Magna Grecia, 88100 Catanzaro, ItalyDepartment of Health Science, University Magna Grecia, 88100 Catanzaro, ItalyDepartment of Medical and Surgical Science, University Magna Grecia, 88100 Catanzaro, ItalyDepartment of Clinical and Experimental Medicine, Nutrition Unit, University Magna Grecia, 88100 Catanzaro, ItalyEpidemiological studies show that fruit consumption may modulate bone mineral density. However, data regarding the effect of the Citrus bergamia Risso (Bergamot orange), a citrus fruit containing a high concentration of flavonoids, on bone health are still lacking. In this study, we investigated the effects of Bergamot polyphenols on the Wnt/β-catenin pathway in two distinct bone cell types (Saos-2 and MG63). Findings showed that exposure to 0.01 and 0.1 mg/mL doses upregulate β-catenin expression (p = 0.001), osteoblast differentiation markers (e.g., RUNX2 and COL1A), and downregulate RANKL (p = 0.028), as compared to the control. Our results highlight, for the first time, that Bergamot polyphenols act on bone cells through the β-catenin pathway. In vivo studies are necessary to fully understand Bergamot’s role against bone resorption.http://www.mdpi.com/2072-6643/10/9/1305polyphenolscitrus fruitsBergamotosteoporosisSaos-2bone cellsβ-catenin
collection DOAJ
language English
format Article
sources DOAJ
author Arturo Pujia
Cristina Russo
Samantha Maurotti
Roberta Pujia
Vincenzo Mollace
Stefano Romeo
Tiziana Montalcini
spellingShingle Arturo Pujia
Cristina Russo
Samantha Maurotti
Roberta Pujia
Vincenzo Mollace
Stefano Romeo
Tiziana Montalcini
Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures
Nutrients
polyphenols
citrus fruits
Bergamot
osteoporosis
Saos-2
bone cells
β-catenin
author_facet Arturo Pujia
Cristina Russo
Samantha Maurotti
Roberta Pujia
Vincenzo Mollace
Stefano Romeo
Tiziana Montalcini
author_sort Arturo Pujia
title Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures
title_short Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures
title_full Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures
title_fullStr Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures
title_full_unstemmed Bergamot Polyphenol Fraction Exerts Effects on Bone Biology by Activating ERK 1/2 and Wnt/β-Catenin Pathway and Regulating Bone Biomarkers in Bone Cell Cultures
title_sort bergamot polyphenol fraction exerts effects on bone biology by activating erk 1/2 and wnt/β-catenin pathway and regulating bone biomarkers in bone cell cultures
publisher MDPI AG
series Nutrients
issn 2072-6643
publishDate 2018-09-01
description Epidemiological studies show that fruit consumption may modulate bone mineral density. However, data regarding the effect of the Citrus bergamia Risso (Bergamot orange), a citrus fruit containing a high concentration of flavonoids, on bone health are still lacking. In this study, we investigated the effects of Bergamot polyphenols on the Wnt/β-catenin pathway in two distinct bone cell types (Saos-2 and MG63). Findings showed that exposure to 0.01 and 0.1 mg/mL doses upregulate β-catenin expression (p = 0.001), osteoblast differentiation markers (e.g., RUNX2 and COL1A), and downregulate RANKL (p = 0.028), as compared to the control. Our results highlight, for the first time, that Bergamot polyphenols act on bone cells through the β-catenin pathway. In vivo studies are necessary to fully understand Bergamot’s role against bone resorption.
topic polyphenols
citrus fruits
Bergamot
osteoporosis
Saos-2
bone cells
β-catenin
url http://www.mdpi.com/2072-6643/10/9/1305
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