Off-line and on-line optical monitoring of microalgal growth

The growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, using classic off-line optical techniques (optical density and fluorescence) and on-line analysis of digital images. In this study, we found that the chlorophyll fluorescence ratio F685/F740 has a linear correlat...

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Main Authors:	Hugo-Enrique Lazcano-Hernández, Gabriela Aguilar, Gabriela Antonia Dzul-Cetz, Rodrigo Patiño, Javier Arellano-Verdejo
Format:	Article
Language:	English
Published:	PeerJ Inc. 2019-11-01
Series:	PeerJ
Subjects:	Chlamydomonas reinhardtii Chlorophyll fluorescence Chlorophyll optical density Fluorescence ratio Chl a fluorescence images C. reinhardtii cultures growth
Online Access:	https://peerj.com/articles/7956.pdf

id	doaj-ed1815c84adc48eeb82b6983f598752f
record_format	Article
spelling	doaj-ed1815c84adc48eeb82b6983f598752f2020-11-25T01:46:40ZengPeerJ Inc.PeerJ2167-83592019-11-017e795610.7717/peerj.7956Off-line and on-line optical monitoring of microalgal growthHugo-Enrique Lazcano-Hernández0Gabriela Aguilar1Gabriela Antonia Dzul-Cetz2Rodrigo Patiño3Javier Arellano-Verdejo4Cátedras CONACYT-El Colegio de la Frontera Sur, Chetumal, Quintana Roo, MéxicoDepartamento de Física Aplicada, Cinvestav Unidad Mérida, Mérida, Yucatán, MéxicoDepartamento de Física Aplicada, Cinvestav Unidad Mérida, Mérida, Yucatán, MéxicoDepartamento de Física Aplicada, Cinvestav Unidad Mérida, Mérida, Yucatán, MéxicoEstación para la Recepción de Información Satelital ERIS-Chetumal, El Colegio de la Frontera Sur, Chetumal, Quintana Roo, MéxicoThe growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, using classic off-line optical techniques (optical density and fluorescence) and on-line analysis of digital images. In this study, we found that the chlorophyll fluorescence ratio F685/F740 has a linear correlation with the logarithmic concentration of microalgae. By using digital images, the biomass concentration correlated with the luminosity of the images through an exponential equation and the length of penetration of a super luminescent blue beam (λ = 440 nm) through an inversely proportional function. The outcomes of this study are useful to monitor both research and industrial microalgae cultures.https://peerj.com/articles/7956.pdfChlamydomonas reinhardtiiChlorophyll fluorescenceChlorophyll optical densityFluorescence ratioChl a fluorescence imagesC. reinhardtii cultures growth
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Hugo-Enrique Lazcano-Hernández Gabriela Aguilar Gabriela Antonia Dzul-Cetz Rodrigo Patiño Javier Arellano-Verdejo
spellingShingle	Hugo-Enrique Lazcano-Hernández Gabriela Aguilar Gabriela Antonia Dzul-Cetz Rodrigo Patiño Javier Arellano-Verdejo Off-line and on-line optical monitoring of microalgal growth PeerJ Chlamydomonas reinhardtii Chlorophyll fluorescence Chlorophyll optical density Fluorescence ratio Chl a fluorescence images C. reinhardtii cultures growth
author_facet	Hugo-Enrique Lazcano-Hernández Gabriela Aguilar Gabriela Antonia Dzul-Cetz Rodrigo Patiño Javier Arellano-Verdejo
author_sort	Hugo-Enrique Lazcano-Hernández
title	Off-line and on-line optical monitoring of microalgal growth
title_short	Off-line and on-line optical monitoring of microalgal growth
title_full	Off-line and on-line optical monitoring of microalgal growth
title_fullStr	Off-line and on-line optical monitoring of microalgal growth
title_full_unstemmed	Off-line and on-line optical monitoring of microalgal growth
title_sort	off-line and on-line optical monitoring of microalgal growth
publisher	PeerJ Inc.
series	PeerJ
issn	2167-8359
publishDate	2019-11-01
description	The growth of Chlamydomonas reinhardtii microalgae cultures was successfully monitored, using classic off-line optical techniques (optical density and fluorescence) and on-line analysis of digital images. In this study, we found that the chlorophyll fluorescence ratio F685/F740 has a linear correlation with the logarithmic concentration of microalgae. By using digital images, the biomass concentration correlated with the luminosity of the images through an exponential equation and the length of penetration of a super luminescent blue beam (λ = 440 nm) through an inversely proportional function. The outcomes of this study are useful to monitor both research and industrial microalgae cultures.
topic	Chlamydomonas reinhardtii Chlorophyll fluorescence Chlorophyll optical density Fluorescence ratio Chl a fluorescence images C. reinhardtii cultures growth
url	https://peerj.com/articles/7956.pdf
work_keys_str_mv	AT hugoenriquelazcanohernandez offlineandonlineopticalmonitoringofmicroalgalgrowth AT gabrielaaguilar offlineandonlineopticalmonitoringofmicroalgalgrowth AT gabrielaantoniadzulcetz offlineandonlineopticalmonitoringofmicroalgalgrowth AT rodrigopatino offlineandonlineopticalmonitoringofmicroalgalgrowth AT javierarellanoverdejo offlineandonlineopticalmonitoringofmicroalgalgrowth
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Off-line and on-line optical monitoring of microalgal growth

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