Low-input ATAC&mRNA-seq protocol for simultaneous profiling of chromatin accessibility and gene expression

Summary: We present a simple, fast, and robust protocol (low-input ATAC&mRNA-seq) to simultaneously generate ATAC-seq and mRNA-seq libraries from the same cells in limited cell numbers by coupling a simplified ATAC procedure using whole cells with a novel mRNA-seq approach that features a seamle...

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Bibliographic Details
Main Authors: Ruifang Li, Sara A. Grimm, Paul A. Wade
Format: Article
Language:English
Published: Elsevier 2021-09-01
Series:STAR Protocols
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166721004706