Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species
An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin g...
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Sociedade Brasileira de Genética
2007-01-01
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doaj-eef47fd0b0ab49eea8aec78b3c4809692020-11-25T02:19:46ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852007-01-013041089109210.1590/S1415-47572007000600010Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish speciesAdriane P. WaskoFábio E. SeverinoFlávia T. PrestiAndréia B. PolettoCesar MartinsAn alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600010actin geneamino acid residuesgene structureNile tilapianucleotide sequenceOreochromis niloticus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Adriane P. Wasko Fábio E. Severino Flávia T. Presti Andréia B. Poletto Cesar Martins |
spellingShingle |
Adriane P. Wasko Fábio E. Severino Flávia T. Presti Andréia B. Poletto Cesar Martins Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species Genetics and Molecular Biology actin gene amino acid residues gene structure Nile tilapia nucleotide sequence Oreochromis niloticus |
author_facet |
Adriane P. Wasko Fábio E. Severino Flávia T. Presti Andréia B. Poletto Cesar Martins |
author_sort |
Adriane P. Wasko |
title |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
title_short |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
title_full |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
title_fullStr |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
title_full_unstemmed |
Partial molecular characterization of the Nile tilapia (Oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
title_sort |
partial molecular characterization of the nile tilapia (oreochromis niloticus) alpha-cardiac muscle actin gene and its relationship to actin isoforms of other fish species |
publisher |
Sociedade Brasileira de Genética |
series |
Genetics and Molecular Biology |
issn |
1415-4757 1678-4685 |
publishDate |
2007-01-01 |
description |
An alpha actin gene segment, isolated from Nile tilapia (Oreochromis niloticus), was characterized by nucleotide sequencing, predicted amino acid sequence and Southern blot hybridization. Genomic DNA amplification resulted in a 1063-bp fragment corresponding to a partial alpha-cardiac muscle actin gene containing exons 3 to 6. Southern blot analysis of the restriction-digested DNA revealed that the Nile tilapia genome contains multiple muscle actin isoforms. Although comparison of the nucleotide sequence, amino acid residues and exon-intron organization of the isolated actin gene with those of other vertebrates showed a high level of identity, diagnostic amino acid residues can still be correlated to distinct actin genes in fish species. |
topic |
actin gene amino acid residues gene structure Nile tilapia nucleotide sequence Oreochromis niloticus |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600010 |
work_keys_str_mv |
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