Effect of freeze-dried Carica papaya leaf juice on inflammatory cytokines production during dengue virus infection in AG129 mice

Abstract Background Carica papaya leaves have been used for traditional treatment of dengue fever and have been reported to exhibit an immunomodulatory activity by affecting the level of cytokine production in vitro and in vivo. Due to the lack of adequate in vivo evidence in dengue disease model, t...

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Main Authors: Nor Azrina Norahmad, Mohd Ridzuan Mohd Abd Razak, Norazlan Mohmad Misnan, Nur Hana Md Jelas, Umi Rubiah Sastu, Amirrudin Muhammad, Tiffiny Chau Dee Ho, Bazilah Jusoh, Nor Azlina Zolkifli, Ravindran Thayan, Adiratna Mat Ripen, Murizal Zainol, Ami Fazlin Syed Mohamed
Format: Article
Language:English
Published: BMC 2019-02-01
Series:BMC Complementary and Alternative Medicine
Subjects:
Online Access:http://link.springer.com/article/10.1186/s12906-019-2438-3
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spelling doaj-ef6bac45f7bf404f8a0ca212370ebb972020-11-25T03:29:11ZengBMCBMC Complementary and Alternative Medicine1472-68822019-02-0119111010.1186/s12906-019-2438-3Effect of freeze-dried Carica papaya leaf juice on inflammatory cytokines production during dengue virus infection in AG129 miceNor Azrina Norahmad0Mohd Ridzuan Mohd Abd Razak1Norazlan Mohmad Misnan2Nur Hana Md Jelas3Umi Rubiah Sastu4Amirrudin Muhammad5Tiffiny Chau Dee Ho6Bazilah Jusoh7Nor Azlina Zolkifli8Ravindran Thayan9Adiratna Mat Ripen10Murizal Zainol11Ami Fazlin Syed Mohamed12Herbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaHerbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaHerbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaHerbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaHerbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaHerbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaHerbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaHerbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaHerbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaInfectious Disease Research Center, Institute for Medical Research, Ministry of Health MalaysiaAllergy and Immunology Research Center, Institute for Medical Research, Ministry of Health MalaysiaHerbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaHerbal Medicine Research Center, Institute for Medical Research, Ministry of Health MalaysiaAbstract Background Carica papaya leaves have been used for traditional treatment of dengue fever and have been reported to exhibit an immunomodulatory activity by affecting the level of cytokine production in vitro and in vivo. Due to the lack of adequate in vivo evidence in dengue disease model, the present study was initiated to screen and identify the cytokines affected by freeze-dried C. papaya leaf juice (FCPLJ) treatment in AG129 mice infected with DEN-2 dengue virus. Methods The AG129 mice were fed orally with FCPLJ for 3 consecutive days after 24 h of dengue virus inoculation. Plasma cytokines were screened by using ProcartaPlex immunoassay. The gene expression in the liver was analyzed by using RT2 Profiler PCR Array. Results The results showed that FCPLJ treatment has increased the plasma CCL2/MCP-1 level during peak of viremia. Gene expression study has identified 8 inflammatory cytokine genes which were downregulated in the liver of infected AG129 mice treated with FCPLJ. The downregulated inflammatory cytokine genes were CCL6/MRP-1, CCL8/MCP-2, CCL12/MCP-5, CCL17/TARC, IL1R1, IL1RN/IL1Ra, NAMPT/PBEF1 and PF4/CXCL4. Conclusion The findings indicated the possible immunomodulatory role of FCPLJ during dengue virus infection in AG129 mice.http://link.springer.com/article/10.1186/s12906-019-2438-3Carica papayaAG129DengueCytokineGene expressionIn vivo
collection DOAJ
language English
format Article
sources DOAJ
author Nor Azrina Norahmad
Mohd Ridzuan Mohd Abd Razak
Norazlan Mohmad Misnan
Nur Hana Md Jelas
Umi Rubiah Sastu
Amirrudin Muhammad
Tiffiny Chau Dee Ho
Bazilah Jusoh
Nor Azlina Zolkifli
Ravindran Thayan
Adiratna Mat Ripen
Murizal Zainol
Ami Fazlin Syed Mohamed
spellingShingle Nor Azrina Norahmad
Mohd Ridzuan Mohd Abd Razak
Norazlan Mohmad Misnan
Nur Hana Md Jelas
Umi Rubiah Sastu
Amirrudin Muhammad
Tiffiny Chau Dee Ho
Bazilah Jusoh
Nor Azlina Zolkifli
Ravindran Thayan
Adiratna Mat Ripen
Murizal Zainol
Ami Fazlin Syed Mohamed
Effect of freeze-dried Carica papaya leaf juice on inflammatory cytokines production during dengue virus infection in AG129 mice
BMC Complementary and Alternative Medicine
Carica papaya
AG129
Dengue
Cytokine
Gene expression
In vivo
author_facet Nor Azrina Norahmad
Mohd Ridzuan Mohd Abd Razak
Norazlan Mohmad Misnan
Nur Hana Md Jelas
Umi Rubiah Sastu
Amirrudin Muhammad
Tiffiny Chau Dee Ho
Bazilah Jusoh
Nor Azlina Zolkifli
Ravindran Thayan
Adiratna Mat Ripen
Murizal Zainol
Ami Fazlin Syed Mohamed
author_sort Nor Azrina Norahmad
title Effect of freeze-dried Carica papaya leaf juice on inflammatory cytokines production during dengue virus infection in AG129 mice
title_short Effect of freeze-dried Carica papaya leaf juice on inflammatory cytokines production during dengue virus infection in AG129 mice
title_full Effect of freeze-dried Carica papaya leaf juice on inflammatory cytokines production during dengue virus infection in AG129 mice
title_fullStr Effect of freeze-dried Carica papaya leaf juice on inflammatory cytokines production during dengue virus infection in AG129 mice
title_full_unstemmed Effect of freeze-dried Carica papaya leaf juice on inflammatory cytokines production during dengue virus infection in AG129 mice
title_sort effect of freeze-dried carica papaya leaf juice on inflammatory cytokines production during dengue virus infection in ag129 mice
publisher BMC
series BMC Complementary and Alternative Medicine
issn 1472-6882
publishDate 2019-02-01
description Abstract Background Carica papaya leaves have been used for traditional treatment of dengue fever and have been reported to exhibit an immunomodulatory activity by affecting the level of cytokine production in vitro and in vivo. Due to the lack of adequate in vivo evidence in dengue disease model, the present study was initiated to screen and identify the cytokines affected by freeze-dried C. papaya leaf juice (FCPLJ) treatment in AG129 mice infected with DEN-2 dengue virus. Methods The AG129 mice were fed orally with FCPLJ for 3 consecutive days after 24 h of dengue virus inoculation. Plasma cytokines were screened by using ProcartaPlex immunoassay. The gene expression in the liver was analyzed by using RT2 Profiler PCR Array. Results The results showed that FCPLJ treatment has increased the plasma CCL2/MCP-1 level during peak of viremia. Gene expression study has identified 8 inflammatory cytokine genes which were downregulated in the liver of infected AG129 mice treated with FCPLJ. The downregulated inflammatory cytokine genes were CCL6/MRP-1, CCL8/MCP-2, CCL12/MCP-5, CCL17/TARC, IL1R1, IL1RN/IL1Ra, NAMPT/PBEF1 and PF4/CXCL4. Conclusion The findings indicated the possible immunomodulatory role of FCPLJ during dengue virus infection in AG129 mice.
topic Carica papaya
AG129
Dengue
Cytokine
Gene expression
In vivo
url http://link.springer.com/article/10.1186/s12906-019-2438-3
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