Investigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform Glioblastoma
Background: Improper prognosis in brain cancers requires new treatments. Using family of purinergic receptors with confirmed apoptotic effect can be beneficial. As the role of type A1 receptor in multiform glioblastoma in relation to the P53 gene and apoptotic pathways is nor reported, we studied th...
Main Authors: | , , , , , , , |
---|---|
Format: | Article |
Language: | fas |
Published: |
Vesnu Publications
2021-02-01
|
Series: | مجله دانشکده پزشکی اصفهان |
Subjects: | |
Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/13007 |
id |
doaj-efbde3ae2ea342f99a130a6d369714c2 |
---|---|
record_format |
Article |
spelling |
doaj-efbde3ae2ea342f99a130a6d369714c22021-06-15T07:44:02ZfasVesnu Publications مجله دانشکده پزشکی اصفهان1027-75951735-854X2021-02-013860187588110.22122/jims.v38i601.130073739Investigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform GlioblastomaFahimeh Zamani-Rarani0Zeinolabedin Shrifian-Dastjerdi1Ali Valiani2Mohammad Zamani-Rarani3Elias Kargar-Abargouei4Ebrahim Eftekhar5Majid Pourentezari6Javad Mohajer-Ansari7PhD Student, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Anatomical Sciences, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IranAssociate Professor, Department of Anatomical Sciences, School of Medicine, Isfahan University of Medical Sciences, Isfahan, IranAssistant Professor, Department of Anatomical Sciences, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IranAssistant Professor, Department of Anatomical Sciences, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IranAssociate Professor, Molecular Medicine Research Center, Hormozgan Health Institute, Hormozgan University of Medical Science, Bandar Abbas, IranAssistant Professor, Department of Biology and Anatomical Sciences, School of Medicine, Shahid Sadoughi University of Medical Sciences, Yazd, IranAssistant Professor, Department of Anatomical Sciences, School of Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IranBackground: Improper prognosis in brain cancers requires new treatments. Using family of purinergic receptors with confirmed apoptotic effect can be beneficial. As the role of type A1 receptor in multiform glioblastoma in relation to the P53 gene and apoptotic pathways is nor reported, we studied the role of agonist (N6-cyclopentyladenosine or CPA) and antagonist (8-cyclopentyl-1,3-dipropylxanthine or DPCPX) of this receptor on cell apoptosis and also expression of P53 genes and caspases 7, 8, and 9. Methods: In this study, MTT assay was used to investigate the rate of cellular proliferation, and flowcytometry method with annexin and Pi was also used to investigate early and late cell apoptosis. To evaluate the internal and external apoptotic pathways expression of P53 genes and caspases 7, 8, and 9, real-time reverse transcription polymerase chain reaction (real-time RT PCR) was used. Findings: The treatment of U87Mg cells with DPCPX increased the expression of P53 gene. Expression of caspase 7 as an executive caspase and caspase 9 as a caspase of the mitochondrial pathway of apoptosis increased, but no expression change was observed in the caspase 8 gene. Conclusion: The results of MTT and flowcytometry showed that DPCPX, in addition to suppressing cell proliferation, stimulated apoptosis in U87Mg cells. Inhibition of adenosine A1 receptors by stimulating the expression of genes involved in apoptotic pathways, especially mitochondrial pathway genes, suppressed cell proliferation and induced apoptosis in U87Mg cells.http://jims.mui.ac.ir/index.php/jims/article/view/13007receptor, adenosine a1apoptosisp53 genes |
collection |
DOAJ |
language |
fas |
format |
Article |
sources |
DOAJ |
author |
Fahimeh Zamani-Rarani Zeinolabedin Shrifian-Dastjerdi Ali Valiani Mohammad Zamani-Rarani Elias Kargar-Abargouei Ebrahim Eftekhar Majid Pourentezari Javad Mohajer-Ansari |
spellingShingle |
Fahimeh Zamani-Rarani Zeinolabedin Shrifian-Dastjerdi Ali Valiani Mohammad Zamani-Rarani Elias Kargar-Abargouei Ebrahim Eftekhar Majid Pourentezari Javad Mohajer-Ansari Investigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform Glioblastoma مجله دانشکده پزشکی اصفهان receptor, adenosine a1 apoptosis p53 genes |
author_facet |
Fahimeh Zamani-Rarani Zeinolabedin Shrifian-Dastjerdi Ali Valiani Mohammad Zamani-Rarani Elias Kargar-Abargouei Ebrahim Eftekhar Majid Pourentezari Javad Mohajer-Ansari |
author_sort |
Fahimeh Zamani-Rarani |
title |
Investigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform Glioblastoma |
title_short |
Investigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform Glioblastoma |
title_full |
Investigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform Glioblastoma |
title_fullStr |
Investigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform Glioblastoma |
title_full_unstemmed |
Investigation the Effect of Adenosine A1 Receptor Agonist and Antagonist on P53 Gene Expression, and Apoptosis Pathways and Rate in U87Mg Multiform Glioblastoma |
title_sort |
investigation the effect of adenosine a1 receptor agonist and antagonist on p53 gene expression, and apoptosis pathways and rate in u87mg multiform glioblastoma |
publisher |
Vesnu Publications |
series |
مجله دانشکده پزشکی اصفهان |
issn |
1027-7595 1735-854X |
publishDate |
2021-02-01 |
description |
Background: Improper prognosis in brain cancers requires new treatments. Using family of purinergic receptors with confirmed apoptotic effect can be beneficial. As the role of type A1 receptor in multiform glioblastoma in relation to the P53 gene and apoptotic pathways is nor reported, we studied the role of agonist (N6-cyclopentyladenosine or CPA) and antagonist (8-cyclopentyl-1,3-dipropylxanthine or DPCPX) of this receptor on cell apoptosis and also expression of P53 genes and caspases 7, 8, and 9.
Methods: In this study, MTT assay was used to investigate the rate of cellular proliferation, and flowcytometry method with annexin and Pi was also used to investigate early and late cell apoptosis. To evaluate the internal and external apoptotic pathways expression of P53 genes and caspases 7, 8, and 9, real-time reverse transcription polymerase chain reaction (real-time RT PCR) was used.
Findings: The treatment of U87Mg cells with DPCPX increased the expression of P53 gene. Expression of caspase 7 as an executive caspase and caspase 9 as a caspase of the mitochondrial pathway of apoptosis increased, but no expression change was observed in the caspase 8 gene.
Conclusion: The results of MTT and flowcytometry showed that DPCPX, in addition to suppressing cell proliferation, stimulated apoptosis in U87Mg cells. Inhibition of adenosine A1 receptors by stimulating the expression of genes involved in apoptotic pathways, especially mitochondrial pathway genes, suppressed cell proliferation and induced apoptosis in U87Mg cells. |
topic |
receptor, adenosine a1 apoptosis p53 genes |
url |
http://jims.mui.ac.ir/index.php/jims/article/view/13007 |
work_keys_str_mv |
AT fahimehzamanirarani investigationtheeffectofadenosinea1receptoragonistandantagonistonp53geneexpressionandapoptosispathwaysandrateinu87mgmultiformglioblastoma AT zeinolabedinshrifiandastjerdi investigationtheeffectofadenosinea1receptoragonistandantagonistonp53geneexpressionandapoptosispathwaysandrateinu87mgmultiformglioblastoma AT alivaliani investigationtheeffectofadenosinea1receptoragonistandantagonistonp53geneexpressionandapoptosispathwaysandrateinu87mgmultiformglioblastoma AT mohammadzamanirarani investigationtheeffectofadenosinea1receptoragonistandantagonistonp53geneexpressionandapoptosispathwaysandrateinu87mgmultiformglioblastoma AT eliaskargarabargouei investigationtheeffectofadenosinea1receptoragonistandantagonistonp53geneexpressionandapoptosispathwaysandrateinu87mgmultiformglioblastoma AT ebrahimeftekhar investigationtheeffectofadenosinea1receptoragonistandantagonistonp53geneexpressionandapoptosispathwaysandrateinu87mgmultiformglioblastoma AT majidpourentezari investigationtheeffectofadenosinea1receptoragonistandantagonistonp53geneexpressionandapoptosispathwaysandrateinu87mgmultiformglioblastoma AT javadmohajeransari investigationtheeffectofadenosinea1receptoragonistandantagonistonp53geneexpressionandapoptosispathwaysandrateinu87mgmultiformglioblastoma |
_version_ |
1721376660904738816 |